TRPM2 Antibody

Images

 
Western Blot: TRPM2 Antibody [NB110-81601] - Lane 1: CHO cells untransfected. Lane 2: CHO cell transfected with rat TRPM2 Lane 3: Mouse cortical neurons (cultured) Photo courtesy of Dr. Paco Herson, Oregon Health & ...read more
Immunocytochemistry/ Immunofluorescence: TRPM2 Antibody [NB110-81601] - TRPM2 antibody was tested in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunocytochemistry/ Immunofluorescence: TRPM2 Antibody [NB110-81601] - Staining of rat hippocampus using NB110-81601. TRPM2 is stained red, GFAP is stained green and nuclei staining is blue. Photo courtesy of Dr. ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.90 mg/ml

TRPM2 Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal portion of the rat TRPM2 protein (within residues 1430-1508). [Swiss-Prot# Q5G856]
Localization
Cell membrane.
Specificity
This is specific for isoform 1, or the long isoform (TRPM2-L), of TRPM2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
Application Notes
This TRPM2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot, where a band is seen at ~181 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
181 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
TRPM2 Peptide (NB110-81601PEP)
Publications
Read Publications using
NB110-81601 in the following applications:

  • 3 publications
  • IHC
    2 publications
  • WB
    4 publications

Reactivity Notes

Mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.90 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRPM2 Antibody

  • EC 3.6.1.13
  • EREG1MGC133383
  • Estrogen-responsive element-associated gene 1 protein
  • KNP3LTrpC-2
  • Long transient receptor potential channel 2
  • LTrpC2
  • LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2
  • NUDT9H
  • NUDT9L1
  • transient receptor potential cation channel, subfamily M, member 2
  • Transient receptor potential channel 7
  • TrpC7

Background

TRP7 (transient receptor protein 7 or TRPC7) is the seventh identified member of mammalian TRPC channel family which includes nonselective cation channels that mediate changes in plasma membrane cation permeability induced by stimulation of PLC (phospholipase C, the cellular integrator that receives input from GPCRs/ RTK). Major cellular function of TRPC channels is to regulate Ca2+ influx across plasma membrane induced by stimulation of these receptors by hormones, neurotransmitters, and growth factors. Localized in the cell membrane and nuclear envelope as multi-pass membrane protein, TRPC7 interacts with with MX1, RNF24, PRKG1 etc. and PRKG1 mediated TRPC7 phosphorylation at Thr-15 site negatively regulates TRPC7's activity. TRP7 has been suggested to form a receptor-activated non-selective calcium permeant cation channel and is operated by a phosphatidylinositol second messenger system activated by RTKs or GPCRs. TRP7 is activated by DAG (diacylglycerol) and also upon intracellular calcium store depletion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-88370
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31133
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-00142
Species: Hu, Mu
Applications: WB, IP, PEP-ELISA
NBP1-70352
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IF
NB100-98844
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NB100-98864
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-12919
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NBP2-12905
Species: Rt
Applications: WB, ICC/IF, IHC, IP
NB100-810
Species: Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-98846
Species: Hu
Applications: WB, IHC, IHC-Fr, IHC-P
NBP1-48036
Species: Hu, Ca, Pm
Applications: WB, IHC, IHC-P
NBP1-97311
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
AF2747
Species: Mu
Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det), ELISA(Sta)
NB110-74925
Species: Mu
Applications: WB, IHC, IHC-Fr, IHC-P
NB110-74960
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-27335
Species: Hu
Applications: WB, Flow, CyTOF-ready
NB100-1617
Species: Hu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-98867
Species: Rt
Applications: WB, IHC
NB110-81601
Species: Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr

Publications for TRPM2 Antibody (NB110-81601)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
WB
(4)
All Applications
Filter By Species
Mouse
(3)
Rat
(4)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB110-81601 Applications Species
Yee AG, Freestone PS, Bai JZ, Lipski J Paradoxical lower sensitivity of Locus Coeruleus than Substantia Nigra pars compacta neurons to acute actions of rotenone. Exp. Neurol. Oct 19 2016 [PMID: 27771354] (IHC) IHC
Rah SY, Kwak JY, Chung YJ, Kim UH ADP-ribose/TRPM2-mediated Ca2+) signaling is essential for cytolytic degranulation and antitumor activity of natural killer cells. Sci Rep 2015 Mar 25 [PMID: 25879940] (ICC/IF, Mouse)

Details:
This publication used the DL550 conjugated form of this antibody (NB110-81601R)
ICC/IF Mouse
Chung MK, Asgar J, Lee J et al. The role of TRPM2 in hydrogen peroxide-induced expression of inflammatory cytokine and chemokine in rat trigeminal ganglia Neuroscience 2015 Apr 04 [PMID: 25849615] (WB, Rat) WB Rat
Lu S, Xiang L, Clemmer JS et al. Oxidative stress increases pulmonary vascular permeability in diabetic rats through activation of transient receptor potential melastatin 2 (TRPM2) channels. Microcirculation 2014 Jul 24 [PMID: 25059284] (WB, Rat)

Details:
TRPM2 antibody used for WB on tissue lysate of pulmonary arteries from control male lean Zucker/LZ Rats and hyperglycemic LZ rats (streptozotocin/STZ 50mg/kg -induced type I diabetic model). Figure 1 shows the down-regulation of TRPM2-L channel in STZ-treated type I diabetic LZ.
WB Rat
Roberge S, Roussel J, Andersson DC et al. TNF-alpha-mediated caspase-8 activation induces ROS production and TRPM2 activation in adult ventricular myocytes. Cardiovasc. Res. 2014 May 06 [PMID: 24802330] (WB, Mouse) WB Mouse
Melzer N, Hicking G, Gobel K, Wiendl H. TRPM2 Cation Channels Modulate T Cell Effector Functions and Contribute to Autoimmune CNS Inflammation PLoS One 2012 [PMID: 23077651] (ICC/IF, Mouse) ICC/IF Mouse
Chung KK, Freestone PS, Lipski J. Expression and functional properties of TRPM2 channels in dopaminergic neurons of the substantia nigra of the rat. J Neurophysiol;106(6):2865-75. 2011 Dec. [PMID: 21900507] (IHC, Rat) IHC Rat
Bai JZ, Lipski J. Differential expression of TRPM2 and TRPV4 channels and their potential role in oxidative stress-induced cell death in organotypic hippocampal culture. Neurotoxicology. 2010 Jan 12. [PMID: 20064552] (WB, ICC/IF, Rat) WB, ICC/IF Rat

Reviews for TRPM2 Antibody (NB110-81601) (0)

There are no reviews for TRPM2 Antibody (NB110-81601). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for TRPM2 Antibody (NB110-81601): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRPM2 Antibody (NB110-81601) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-81601AF405
Alexa Fluor 488 Labeled NB110-81601AF488
Alexa Fluor 647 Labeled NB110-81601AF647
Alexa Fluor 700 Labeled NB110-81601AF700
Biotin Labeled NB110-81601B
DyLight 405 Labeled NB110-81601V
DyLight 488 Labeled NB110-81601G
DyLight 550 Labeled NB110-81601R
DyLight 650 Labeled NB110-81601C
FITC Labeled NB110-81601F
HRP Labeled NB110-81601H

Additional TRPM2 Products

Bioinformatics Tool for TRPM2 Antibody (NB110-81601)

Discover related pathways, diseases and genes to TRPM2 Antibody (NB110-81601). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRPM2 Antibody (NB110-81601)

Discover more about diseases related to TRPM2 Antibody (NB110-81601).
 

Pathways for TRPM2 Antibody (NB110-81601)

View related products by pathway.

PTMs for TRPM2 Antibody (NB110-81601)

Learn more about PTMs related to TRPM2 Antibody (NB110-81601).
 

Research Areas for TRPM2 Antibody (NB110-81601)

Find related products by research area.

Blogs on TRPM2.

Winter is coming, and TRPM8 welcomes the cold!
TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds.  While TRPM8 is best known for its location in peripheral nerve endings, it has functio...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TRPM2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TRPM2