TRPC4 Antibody

Western Blot: TRPC4 Antibody [NBP1-20989] - Staining of Rat Brain lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989] - Staining of paraffin embedded Human Placenta with antibody at 4.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989] - Staining of paraffin embedded Human Kidney with antibody at 4.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt, Mu, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRPC4 Antibody Summary

• Description: Novus Biologicals Goat TRPC4 Antibody (NBP1-20989) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-TRPC4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KHAKEEDSSIDYD corresponding to internal region (near C-Terminus) according to NP_003297.1, NP_001129427.1, NP_001129429.1, NP_001129428.1, NP_001129430.1.
• Epitope: C terminus
• Specificity: This antibody is expected to recognise all six reported isoforms (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1).
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRPC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4.75 ug/mL
  • Immunohistochemistry-Paraffin 4.75 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRPC4 Antibody

• hTrp4
• hTrp-4
• MGC119570
• MGC119571
• MGC119572
• MGC119573
• short transient receptor potential channel 4
• transient receptor potential 4
• transient receptor potential cation channel, subfamily C, member 4
• TRP4HTRP4
• TRPC4
• Trp-related protein 4

Background

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective . May also be activated by intracellular calcium store depletion

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPC4 Antibody (NBP1-20989)(2)

Publications using NBP1-20989

• Abou-Saleh H, Pathan AR, Daalis A et al. Inositol 1,4,5-Trisphosphate (IP3) Receptor Up-regulation in Hypertension Is Associated with Sensitization of Ca2+ Release and Vascular Smooth Muscle Contractility. J Biol Chem. 2013-11-15 [PMID: 24097979] (WB, Rat)
• Song X, Zhao Y, Narcisse L et al. Canonical transient receptor potential channel 4 (TRPC4) co-localizes with the scaffolding protein ZO-1 in human fetal astrocytes in culture. Glia 2005-02-01 [PMID: 15540229]

Bioinformatics

• Gene Symbol: TRPC4
• Entrez:
  • 7223(Human)
  • 22066(Mouse)
  • 84494(Rat)
• Uniprot:
  • NP_057263.1(Human)