TRPC6 Antibody

Images

 
Western Blot: TRPC6 Antibody [NBP1-77260] - Mouse lung tissue lysate with TRPC6 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TRPC6 Antibody [NBP1-77260] - Immunofluorescence of TRPC6 in Mouse Lung cells with TRPC6 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: TRPC6 Antibody [NBP1-77260] - Staining of TRPC6 in mouse lung tissue with TRPC6 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: TRPC6 Antibody [NBP1-77260] - Mouse lung tissue with TRPC6 antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TRPC6 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TRPC6. The immunogen is located within the last 50 amino acids of TRPC6.
Specificity
This antibody may cross-react with TRPC3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPC6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
TRPC6 Peptide (NBP1-77260PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRPC6 Antibody

  • FLJ11098
  • FSGS2
  • short transient receptor potential channel 6
  • transient receptor potential cation channel, subfamily C, member 6
  • Transient receptor protein 6
  • TRP-6
  • TRP6FLJ14863
  • TrpC6

Background

The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, TRPC6 acts as a non-selective store-independent receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that TRPC6 encodes endogenous DAG-activated receptor-operated cation channels in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRPC6 Antibody (NBP1-77260). (Showing 1 - 1 of 1 FAQ).

  1. I have tested this antibody and would like to share the results from my first preliminary experiment. This Ab has been tested in ICC using a human cell line. Blocking buffer contains either goat or donkey serum. Dilutions: both 1:1000 and 1500 have been tested. Secondary ab is goat anti rabbit FITC (1:400). Results: every single cell shows VERY WEAK signal around only the cell body. No signal was detected in any cytoplasmic processes, which is really different from TRPM2. As the signal is weak, I will increase the concentration by using the following dilutions instead: 1: 200 and 1:500 to find out if the staining is getting stronger. Questions: Despite the weak staining, every single cell has been stained. Thus, (1) How can I confirm that the staining is specific? (2) How can confirm the staining is in the cell membrane?
    • Membrane staining should be around the edge of the cell. If this is not what you are seeing then I would suspect that your staining is unsuccessful. You may need to permeabilize since this antibody is expected to stain the intracellular portion of TRPC6 and what you're seeing may simply be background and non-specific. Once again, confirmation of staining should best be discussed with your mentor who should have greater knowledge on your proteins of interest than I do.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRPC6
Entrez
Uniprot