TRPM5 Antibody

• Reactivity: Rt, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

TRPM5 Antibody Summary

• Immunogen: A synthetic peptide from the 4th extracellular loop of rat TRPM5 conjugated to an immunogenic carrier protein was used as the immunogen.
• Specificity: TRPM5.
• Predicted Species: Mouse (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPM5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1: 300 - 1: 2000
  • Immunohistochemistry 1: 300 - 1: 2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for TRPM5 Antibody

• Long transient receptor potential channel 5
• LTrpC5
• LTrpC-5
• LTRPC5transient receptor potential cation channel subfamily M member 5
• MLSN1- and TRP-related gene 1 protein
• MTR1MLSN1 and TRP-related
• transient receptor potential cation channel, subfamily M, member 5

Background

FUNCTION: Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPM5 Antibody (NB100-98867)(1)

Publication using NB100-98867

• Delgiorno Kathleen E, Hall Jason C, Takeuchi Kenneth K et al. Identification and manipulation of biliary metaplasia in pancreatic tumors. Gastroenterology. 2014 Jan 01 [PMID: 23999170]

Bioinformatics

• Gene Symbol: TRPM5
• Entrez:
  • 365391(Rat)