TRPM7 Antibody

Western Blot: TRPM7 Antibody [NBP1-46829] - Whole cell lysate (50 ug) from K-562, MCF-7, HeLa, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRPM7 antibody used for WB at 1 ug/ml. Detection: hemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: TRPM7 Antibody [NBP1-46829] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRPM7 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TRPM7 was also immunoprecipitated by rabbit anti-TRPM7 antibody BL9785, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1 minute (B).

Immunoprecipitation: TRPM7 Antibody [NBP1-46829] - Detection of human TRPM7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRPM7 antibody NBP1-46829 (lot NBP1-46829-3) used for IP at 6 ug per reaction. TRPM7 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46829-2). For blotting immunoprecipitated TRPM7, NBP1-46829 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TRPM7 Antibody Summary

• Description: Novus Biologicals Rabbit TRPM7 Antibody (NBP1-46829) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 1815 and 1865 of human transient receptor potential cation channel, subfamily M, member 7 (NP_060142.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPM7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRPM7 Antibody

• CHAK
• CHAK1FLJ25718
• Channel-kinase 1
• EC 2.7.11.1
• Long transient receptor potential channel 7
• LTRPC ion channel family member 7
• LTrpC7
• LTrpC-7
• LTRPC7FLJ20117
• transient receptor potential cation channel subfamily M member 7
• transient receptor potential cation channel, subfamily M, member 7
• transient receptor potential-phospholipase C-interacting kinase
• TRP-PLIK

Background

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPM7
• Entrez:
  • 54822(Human)
• Uniprot:
  • Q96QT4(Mouse)