TRPM7 Antibody - BSA Free

• Reactivity: Hu, Mu(-)
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRPM7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRPM7 Antibody - BSA Free (NB500-243) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to the C-terminal region of rat TRPM7. [UniProt# Q925B3]
• Localization: Cell Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPM7
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 5 ul
  • Western Blot 1:1000
• Application Notes: This TRPM7 antibody is useful for Western blot on immunoprecipitated samples only, does not work on endogenous samples tested to date. The investigator should determine the optimal working dilution.
• Control Peptide:

  TRPM7 Peptide (NB500-243PEP)

Reactivity Notes

It does not work on endogenous samples tested for western analysis (human, rat and mouse cell lines).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for TRPM7 Antibody - BSA Free

• CHAK
• CHAK1FLJ25718
• Channel-kinase 1
• EC 2.7.11.1
• Long transient receptor potential channel 7
• LTRPC ion channel family member 7
• LTrpC7
• LTrpC-7
• LTRPC7FLJ20117
• transient receptor potential cation channel subfamily M member 7
• transient receptor potential cation channel, subfamily M, member 7
• transient receptor potential-phospholipase C-interacting kinase
• TRP-PLIK

Background

Melastatin-like transient receptor potential (TRPM) subfamilies are a diverse group of voltage-independent Ca2+-permeable cation channels expressed in mammalian cells and among them, TRPM6 /TRPM7 are unique as they possess an enzyme domain in their C termini and are regulated by intracellular levels of Mg2+-complexed nucleotides. TRPM7 is a multi-pass membrane essential ion channel and serine/threonine-protein kinase which is a divalent cation channel permeable to Ca2+/Mg2+ ions. TRPM7 plays a key role in Mg2+ ion homeostasis and in the regulation of anoxic neuronal cell death. It also participates in maintaining the plasma membrane divalent cation fluxes according to cell's metabolic state. TRPM7 interacts with PLCB1 for generating TRPM6/7 heterodimers and TRPM6/7 are widely expressed ion channels associated with cell proliferation and survival. TRPM6/7 mutations have been linked to a hereditary form of hypomagnesaemia caused by impaired Mg2+ reabsorption. Moreover, defective TRPM7 causes increased susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TRPM7 Antibody (NB500-243). (Showing 1 - 1 of 1 FAQ).

1. What is the concentration of TRPM7 Antibody, catalog# NB500-243, Lot# I6111?
   • This TRPM7 antibody (catalog# NB500-243) is an unpurified rabbit polyclonal which we offer as whole antisera format. Accordingly,  concentration is not relevant to this product. However, the total protein concentration of unpurified whole sera generally ranges between 15 to 20 mg/ml.

Bioinformatics

• Gene Symbol: TRPM7
• Entrez:
  • 54822(Human)
  • 58800(Mouse)