TRF-2 Products

Antibodies
TRF-2 Antibody
TRF-2 Antibody
NB100-56694
Species: Hu
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP
Host: Goat Polyclonal
Lysates
TRF-2 293T Cell Transient Ove ...
TRF-2 293T Cell Transient Overexpr...
H00007014-T01
Species: Hu
Applications: WB
Proteins
Recombinant Human TRF-2 GST ( ...
Recombinant Human TRF-2 GST (N-Ter...
H00007014-P01
Species: Hu
Applications: WB, ELISA, PA, AP
TRF-2 Recombinant Protein Ant ...
TRF-2 Recombinant Protein Antigen
NBP1-86911PEP
Species: Hu
Applications: AC
TRF-2 Recombinant Protein Ant ...
TRF-2 Recombinant Protein Antigen
NBP1-86910PEP
Species: Hu
Applications: AC

Description

Originally discovered as part of shelterin complex, telomeric repeat-binding factor 2 (TRF2, also called TERF2 or TRB2) is a ubiquitously expressed nuclear protein (55-60 kDa) involved in telomere homeostasis. TRF2 contains an N-terminal GAR domain, a central TRFH dimerization domain, and a C-terminal SAND/MYB-type DNA binding domain. Trf2 RNA has 10 exons and alternative splicing in rodents produces a truncated form, TRF2-S, which lacks the DNA binding domain and nuclear localization signal (NLS) (1).

Both TRF2 and TRF1 bind to telomeric double stranded 5'-TTAGGG-3' DNA repeats, then recruit RAP1, TIN2, TPP1, and POT1 for the assembly of the shelterin complex. The telomeric association of TRF2 is greatly increased in the S phase of the cell cycle (2). Loss of TRF2 leads to telomere shortening, the DNA damage response, chromosomal instability, and replicative senescence. Interestingly, the contribution of TRF2 to telomere shortening via a telomerase-independent mechanism has also been reported (3). In conjunction with the exonuclease, Apollo, TRF2 protects telomeres during replication and negatively regulates the accumulation of DNA topoisomerase (TOP1, TOP2A and TOP2B).

TRF2 has been implicated in cancer, shown to be a major oncogene in telomerase-deficient mice. A link to Werner syndrome, a premature aging disease caused by the loss of WRN, has been reported based on TRF2 recruitment of WRN for processing of telomeric DNA (4). TRF2 expression is increased during human embryonic stem cell differentiation and has been shown to interact with Repressor Element-1 Silencing Transcription Factor (REST), protecting it from proteasomal degradation (5).

References

1. Grammatikakis, I., Zhang, P., Mattson, M. P., & Gorospe, M. (2016). The long and the short of TRF2 in neurogenesis. Cell cycle (Georgetown, Tex.), 15(22), 3026-3032. PMID: 27565210

2. Li, F., Kim, H., Ji, Z., Zhang, T., Chen, B., Ge, Y., Hu, Y., Feng, X., Han, X., Xu, H., Zhang, Y., Yu, H., Liu, D., Ma, W., & Songyang, Z. (2018). The BUB3-BUB1 Complex Promotes Telomere DNA Replication. Molecular cell, 70(3), 395-407. PMID: 29727616

3. Ancelin, K., Brunori, M., Bauwens, S., Koering, C. E., Brun, C., Ricoul, M., Pommier, J. P., Sabatier, L., & Gilson, E. (2002). Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2. Molecular and cellular biology, 22(10), 3474-3487. PMID: 11971978

4. Machwe A, Xiao L, & Orren DK. (2004) TRF2 recruits the Werner syndrome (WRN) exonuclease for processing of telomeric DNA. Oncogene. 23(1):149-56. PMID: 14712220.

5. Diotti, R., & Loayza, D. (2011). Shelterin complex and associated factors at human telomeres. Nucleus (Austin, Tex.), 2(2), 119-135. PMID: 21738835

Bioinformatics

Entrez Human
Mouse
Rat
Uniprot Human
Human
Human
Product By Gene ID 7014
Alternate Names
  • Telomeric repeat binding protein 2
  • TRF2
  • TTAGGG repeat binding factor 2
  • TERF2
  • TRBF2
  • telomeric repeat-binding factor 2
  • Telomeric DNA-binding protein
  • TTAGGG repeat-binding factor 2
  • telomeric repeat binding factor 2

Research Areas for TRF-2

Find related products by research area and learn more about each of the different research areas below.

Cancer
Cellular Markers
Chromatin Research
Transcription Factors and Regulators
Tumor Suppressors

PTMs for TRF-2

Learn more about PTMs related to TRF-2.

Phosphorylation
Methylation
Cleavage
Acetylation
Ubiquitination

Bioinformatics Tool for TRF-2

Discover related pathways, diseases and genes to TRF-2. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
 
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Related TRF-2 Blog Posts

Check out the latest blog posts on TRF-2.
Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...    Read more.
TRF2: Telomere Maintenance and Protection
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an...    Read more.
Read more TRF-2 related blogs.