TNF-alpha Antibody - BSA Free


Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was more
Western Blot: TNF-alpha Antibody [NBP1-19532] - Scutellarin attenuates hypertension-induced brain expression of NF-kappaB, TNF-alpha, IL-1beta, and IL-18. Western immunoblot analysis for TNF-alpha in the rat cortex and more
Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to more
Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to more

Product Details

Reactivity Hu, Mu, Rt, Ca, Fi, Pm, Fe, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC
BSA Free
1.0 mg/ml

Order Details

TNF-alpha Antibody - BSA Free Summary

A synthetic peptide made to an internal portion of the human TNF alpha protein (between residues 100-200) [Uniprot: P01375]
Predicted Species
Primate (100%), Monkey (98%), Feline (90%), Baboon (97%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:300
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:250 - 1:500
Application Notes
Western Blot data from customer review and citation (PMID: 24223475). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 26219646). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26725948). Use in FLOW reported in scientific literature (PMID: 26889045).
Reviewed Applications
Read 3 Reviews rated 5
NBP1-19532 in the following applications:

Read Publications using
NBP1-19532 in the following applications:

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 29321977). Immunogen displays the following percentage of sequence identity for non-tested species: porcine (89%), equine (87%), and guinea pig (84%). Use in Canine reported in scientific literature (PMID:32656339).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for TNF-alpha Antibody - BSA Free

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


Tumor necrosis factor (TNF)-alpha is a pro-inflammatory cytokine belonging to the TNF superfamily that is secreted by monocytes/macrophages, T cell, and natural killer (NK), among others (1). TNF-alpha is synthesized as a 233 amino acid (aa) transmembrane protein (mTNF-alpha) with a theoretical molecular weight (MW) of 26 kDa (1,2) that forms a homotrimer. mTNF-alpha is cleaved by TNF-alpha converting enzyme (TACE) and released in its 157 aa, 17 kDa soluble form (sTNF-alpha) (1-5). Both mTNF-alpha and sTNF-alpha are capable of binding type 1 TNF receptors (TNFR1), whereas mTNF-alpha predominately binds to TNFR2 (1,2). TNF-alpha binding to its receptors causes receptor recruitment of adaptor proteins, formation of signaling complexes, and downstream signaling cascades (e.g. MAPK, NF-kappaB, and Caspase-8), leading to distinct cellular responses such as survival, proliferation, inflammation, necroptosis, and apoptosis (1-5).

TNF-alpha is critical for normal immune response; however, dysregulation of TNF-alpha production can result in various pathologies (2,4,5). Excessive production of pro-inflammatory cytokines including interleukin 1 (IL-1), IL-6, and TNF-alpha has been implicated in an array of autoimmune diseases like rheumatoid arthritis (RA), inflammatory bowel disease (IBD), and psoriasis (2,4,5). Anti-TNF monoclonal antibodies, including Infliximab, and soluble TNFR have been approved for the treatment of autoimmune and TNF-mediated diseases (5). Additionally, data suggests that TNF inhibitors can be beneficial for treating patients experiencing immune-related adverse events associated with immune checkpoint inhibitor cancer treatment (6).


1. Holbrook J, Lara-Reyna S, Jarosz-Griffiths H, McDermott M. Tumour necrosis factor signalling in health and disease. F1000Res. 2019;8:F1000 Faculty Rev-111.

2. Jang DI, Lee AH, Shin HY, et al. The Role of Tumor Necrosis Factor Alpha (TNF-alpha) in Autoimmune Disease and Current TNF-alpha Inhibitors in Therapeutics. Int J Mol Sci. 2021;22(5):2719.

3. Horiuchi T, Mitoma H, Harashima S, Tsukamoto H, Shimoda T. Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology (Oxford). 2010;49(7):1215-1228.

4. Webster JD, Vucic D. The Balance of TNF Mediated Pathways Regulates Inflammatory Cell Death Signaling in Healthy and Diseased Tissues. Front Cell Dev Biol. 2020;8:365.

5. Kalliolias GD, Ivashkiv LB. TNF biology, pathogenic mechanisms and emerging therapeutic strategies. Nat Rev Rheumatol. 2016; 12(1):49-62.

6. Chen AY, Wolchok JD, Bass AR. TNF in the era of immune checkpoint inhibitors: friend or foe?. Nat Rev Rheumatol. 2021;17(4):213-223. doi:10.1038/s41584-021-00584-4


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Ca, Hu, Pm, Mu, Rt
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Applications: WB, Flow, ICC/IF, IHC, IHC

Publications for TNF-alpha Antibody (NBP1-19532)(70)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Fish.

We have publications tested in 8 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-FrFl, IHC-P, WB.

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Showing Publications 1 - 10 of 70. Show All 70 Publications.
Publications using NBP1-19532 Applications Species
Abdelsalam M, El-Mahdy N, Abou-Saif S Direct-acting antivirals sofosbuvir and daclatasvir attenuate CCl4-induced liver fibrosis in mice Liver Research 2023-02-01 (IHC, Mouse) IHC Mouse
Wei F, Tuong ZK, Omer M et al. A novel multifunctional radioprotective strategy using P7C3 as a countermeasure against ionizing radiation-induced bone loss Bone research 2023-06-29 [PMID: 37385982] (IHC-Fr, Rat) IHC-Fr Rat
Bouamar H, Broome L, Lathrop K et al. mTOR Inhibition Abrogates Human Mammary Stem Cells and Early Breast Cancer Progression Markers SSRN Electronic Journal 2022-07-10 (FLOW, Human) FLOW Human
Pasupulati A, Nishad R, Mukhi D et al. Growth hormone induces TNF-alpha in podocytes and contributes to monocyte-to-macrophage differentiation: Implications in Diabetic kidney disease Research Square 2022-02-02
Rayilla R, Phanithi P Traumatic Brain Injury: Crosstalk Between ER and Mitochondria Contributes to Oligodendrocyte Cell Death and Demyelination Research Square 2021-12-06 (IHC-P, Rat) IHC-P Rat
Vos S, Aaron R, Weng M et al. CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression Investigative ophthalmology & visual science 2023-06-01 [PMID: 37294707] (ICC/IF, Human) ICC/IF Human
Alam A, Singh T, Kayhanian S et al. Modelling the inflammatory response of traumatic brain injury using human induced pluripotent stem cell derived microglia Journal of neurotrauma 2023-06-01 [PMID: 37261979]
Aswani SS, Mohan MS, Aparna NS et al. Oxidized LDL-mediated upregulation of ADAMTS-4 in monocytes/macrophages involves ROS-NF-?B-SIRT-1 pathway Physiology international 2023-05-22 [PMID: 37216221]
Show All 70 Publications.

Reviews for TNF-alpha Antibody (NBP1-19532) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Rat, Other.

Reviews using NBP1-19532:
Filter by Applications
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Images Ratings Applications Species Date Details
reviewed by:
Nicholas Izzo
ICC/IF Rat 10/13/2016


Sample TestedPrimary neurons
Immunofluorescence TNF-alpha NBP1-19532
reviewed by:
Anonymous -
IF Other 01/07/2016


Sample Testedtrabecular meshwork (TM) region of pig eyes
Western Blot TNF-alpha NBP1-19532
reviewed by:
Verified Customer
WB Human 07/24/2014


ApplicationWestern Blot
Sample TestedRecombinant his-tagged TNFa made in E. coli cells

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TNF-alpha Antibody (NBP1-19532). (Showing 1 - 7 of 7 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
  5. We ordered a TNF alpha antibody from you back in May, and I am inquiring about the expiration date. The antibody we ordered was cat #NBP1-19532 and lot # 360986. . The data sheet says that short term storage at 4C and -20C for long term storage but that they are only guaranteed for 6 months. My question is are you considering the "short term storage" at 4C to be the 6 month expiration and -20C the antibody should still be OK? Typically we give our antibodies that are stored at -20C a two year expiration if one is not given.
    • We suggest to store the antibody at 4 degrees for no longer than a week. After this the product should be stored at -20 degrees and we are not sure if this is can be used after the 6 month period. It is likely that the antibody should be fine however we only guarantee our products for up to 6 months.
  6. Is there any positive control tissue besides human colon carcinoma tissue that can be used for for paraffin embedded IHC testing?
    • You can use skin, lung, liver or mammary tumor tissue etc as a positive control for your assay.
  7. I would like to learn about the TNF alpha antibody (NBP1-19532) immunohistochemistry protocol. Is there any specific protocol you have?
    • Our lab did not use any customized protocol for the IHC-P validation of this antibody. Please see our general IHC-P protocol.

Secondary Antibodies


Isotype Controls

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Blogs on TNF-alpha. Showing 1-10 of 12 blog posts - Show all blog posts.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.
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Recent Reviews


Nicholas Izzo
Application: ICC/IF
Species: Rat

Anonymous -
Application: IF
Species: Other

Verified Customer
Application: WB
Species: Human


Gene Symbol TNF