TNF-alpha Antibody


Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was more
Western Blot: TNF-alpha Antibody [NBP1-19532] - Scutellarin attenuates hypertension-induced brain expression of NF-kappaB, TNF-alpha, IL-1beta, and IL-18. Western immunoblot analysis for TNF-alpha in the rat cortex and more
Immunocytochemistry/ Immunofluorescence: TNF-alpha Antibody [NBP1-19532] - TNF alpha antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight more
Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to more
Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to more

Product Details

Reactivity Hu, Mu, Rt, Fi, Pm, Ca, Fe, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
1.0 mg/ml

TNF-alpha Antibody Summary

A synthetic peptide made to an internal portion of the human TNF alpha protein (between residues 100-200) [Uniprot: P01375]
Predicted Species
Primate (100%), Monkey (98%), Canine (94%), Feline (90%), Baboon (97%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:250 - 1:500
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:300
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunohistochemistry Free-Floating
Application Notes
Western Blot data from customer review and citation (PMID: 24223475). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 26219646). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26725948). Use in FLOW reported in scientific literature (PMID: 26889045).
Reviewed Applications
Read 3 Reviews rated 5
NBP1-19532 in the following applications:

Read Publications using
NBP1-19532 in the following applications:

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 29321977). Immunogen displays the following percentage of sequence identity for non-tested species: porcine (89%), equine (87%), and guinea pig (84%).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for TNF-alpha Antibody

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


Tumor Necrosis Factor Alpha (TNF alpha) is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages and causes tumor necrosis when injected into tumour bearing mice. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid etabolism, and coagulation and it has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the europrotective function of this cytokine. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
Species: Hu
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu, Mu, Rt, Po
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
Species: Hu, Mu, Rt, Fi, Pm, Ca, Fe, Pm, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl

Publications for TNF-alpha Antibody (NBP1-19532)(30)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Fish.

We have publications tested in 7 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, WB.

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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NBP1-19532 Applications Species
Han Y, Lee Y, Jang Y et Al. Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARdelta-AMPK-PGC-1 alpha Pathway in Dyslipidemic Conditions BioMed Research International Mar 19 2020 [PMID: 32258142] (WB, Human, Mouse) WB Human, Mouse
Wang M, Zong Hf, Chang Kw et Al. 5-HT1AR alleviates A beta-induced cognitive decline and neuroinflammation through crosstalk with NF-kappa B pathway in mice Int. Immunopharmacol. Jan 1 2020 [PMID: 32143008] (IHC, Mouse) IHC Mouse
Fu XQ, Peng J, Wang AH, Luo ZG Tumor necrosis factor alpha mediates neuromuscular synapse elimination Cell Discov Mar 3 2020 [PMID: 32140252] (IHC, Mouse) IHC Mouse
Wang M, Law ME, Davis BJ, et al. Identification of hub genes and key pathways of dietary advanced glycation end products induced non alcoholic fatty liver disease by bioinformatics analysis and animal experiments Mol Med Report Dec 9 2019 [PMID: 31974594] (IHC, Mouse) IHC Mouse
Li X, Xue YM, Guo HM et al. High hydrostatic pressure induces atrial electrical remodeling through upregulation of inflammatory cytokines Life Sci. Dec 20 2019 [PMID: 31870776] (WB, Mouse, Rat) WB Mouse, Rat
Sparks RP, Lui A, Bader D et al. A specific small-molecule inhibitor of Protein Kinase CdeltaI activity improves metabolic dysfunction in human adipocytes from obese individuals J. Biol. Chem. Aug 14 2019 [PMID: 31413114] (WB, Human) WB Human
Li S, Jiang L, Yang Y et al. Siglec1 enhances inflammation through miR-1260-dependent degradation of I kappa B alpha in COPD Exp. Mol. Pathol. Jan 30 2020 [PMID: 32007531] (WB, Rat) WB Rat
Wu J, Dou Y, Ladiges W Adverse neurological effects of short-term sleep deprivation in aging mice are prevented by SS31 peptide bioRxiv Jun 6 2020 (WB, Mouse) WB Mouse
Naranjo JD, Saldin LT, Sobieski E et al. Esophageal extracellular matrix hydrogel mitigates metaplastic change in a dog model of Barrett's esophagus Sci Adv Jul 1 2020 [PMID: 32656339] (WB, Canine) WB Canine
Show All 30 Publications.

Reviews for TNF-alpha Antibody (NBP1-19532) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Rat, Other.

Reviews using NBP1-19532:
Filter by Applications
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Images Ratings Applications Species Date Details
reviewed by:
ICC/IF Rat 10/13/2016


Sample TestedPrimary neurons
Immunofluorescence TNF-alpha NBP1-19532
reviewed by:
IF Other 01/07/2016


Sample Testedtrabecular meshwork (TM) region of pig eyes


Blocking Details-

Primary Anitbody

Dilution Ratio2 ug/ml

Secondary Antibody

Secondary DescriptionAlexa 594


Detection Notes-
Fixation Details2% paraformaldehyde fixed for 20 minutes


CommentsWe used fresh pig eyes, then cut and keep trabecular meshwork (TM) region and 2 percent paraformaldehyde fixed for 20 minutes, then standard IHC was carried out.
Western Blot TNF-alpha NBP1-19532
reviewed by:
Ranveer Singh
WB Human 07/24/2014


ApplicationWestern Blot
Sample TestedRecombinant his-tagged TNFa made in E. coli cells


Blocking Details5% BSA in TBST for 1 h

Primary Anitbody

Dilution Ratio1:2000, Overnight at 4 degree in TBST on a rocker

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG HRP (Biorad)
Secondary Manufacturer Cat#170-5046
Secondary Concentration1:20,000 in 5% BSA


Detection NotesECL reagent was used. Exposure for 5 sec

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TNF-alpha Antibody (NBP1-19532). (Showing 1 - 7 of 7 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
  5. We ordered a TNF alpha antibody from you back in May, and I am inquiring about the expiration date. The antibody we ordered was cat #NBP1-19532 and lot # 360986.. The data sheet says that short term storage at 4C and -20C for long term storage but that they are only guaranteed for 6 months. My question is are you considering the "short term storage" at 4C to be the 6 month expiration and -20C the antibody should still be OK? Typically we give our antibodies that are stored at -20C a two year expiration if one is not given.
    • We suggest to store the antibody at 4 degrees for no longer than a week. After this the product should be stored at -20 degrees and we are not sure if this is can be used after the 6 month period. It is likely that the antibody should be fine however we only guarantee our products for up to 6 months.
  6. Is there any positive control tissue besides human colon carcinoma tissue that can be used for for paraffin embedded IHC testing?
    • You can use skin, lung, liver or mammary tumor tissue etc as a positive control for your assay.
  7. I would like to learn about the TNF alpha antibody (NBP1-19532) immunohistochemistry protocol. Is there any specific protocol you have?
    • Our lab did not use any customized protocol for the IHC-P validation of this antibody. Please see our general IHC-P protocol.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-19532AF350
Alexa Fluor 405 NBP1-19532AF405
Alexa Fluor 488 NBP1-19532AF488
Alexa Fluor 532 NBP1-19532AF532
Alexa Fluor 594 NBP1-19532AF594
Alexa Fluor 647 NBP1-19532AF647
Alexa Fluor 700 NBP1-19532AF700
Alexa Fluor 750 NBP1-19532AF750
Biotin NBP1-19532B
DyLight 350 NBP1-19532UV
DyLight 405 NBP1-19532V
DyLight 488 NBP1-19532G
DyLight 550 NBP1-19532R
DyLight 594 NBP1-19532DL594
DyLight 650 NBP1-19532C
DyLight 680 NBP1-19532FR
DyLight 755 NBP1-19532IR
FITC NBP1-19532F
HRP NBP1-19532H
Janelia Fluor 549 NBP1-19532JF549
Janelia Fluor 646 NBP1-19532JF646

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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Recent Reviews


Application: ICC/IF
Species: Rat

Application: IF
Species: Other

Ranveer Singh
Application: WB
Species: Human


Gene Symbol TNF