TNF-alpha Antibody - BSA Free

Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNF alpha in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: TNF-alpha Antibody [NBP1-19532] - Scutellarin attenuates hypertension-induced brain expression of NF-kappaB, TNF-alpha, IL-1beta, and IL-18. Western immunoblot analysis for TNF-alpha in the rat cortex and striatum. Treatment with scutellarin significantly reduced the expression of these inflammatory markers in a dose-dependent manner. *P < 0.001 versus sham group; #P < 0.05, P < 0.001 versus NS group; &P < 0.001 versus low-dose group. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/mi/2013/432623/), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected diffused immunostaining of this protein in the tested tissue. Staining was primarily observed in the epithelial cells and some cells showed membrane positivity also.

Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected immunopositivity of this protein in the tested tissue. Staining was primarily observed in the epithelial cells while some staining was present in mucosa muscularis also.

• Reactivity: Hu, Mu, Rt, Ca, Fi, Pm, Fe, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TNF-alpha Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human TNF alpha protein (between residues 100-200) [Uniprot: P01375]
• Localization: Membrane
• Predicted Species: Primate (100%), Monkey (98%), Feline (90%), Baboon (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:300
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:250 - 1:500
• Application Notes: Western Blot data from customer review and citation (PMID: 24223475). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 26219646). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 26725948). Use in FLOW reported in scientific literature (PMID: 26889045).

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 29321977). Immunogen displays the following percentage of sequence identity for non-tested species: porcine (89%), equine (87%), and guinea pig (84%). Use in Canine reported in scientific literature (PMID:32656339).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TNF-alpha Antibody - BSA Free

• APC1 protein
• Cachectin
• Cachetin
• DIF
• TNF
• TNF, monocyte-derived
• TNFA
• TNF-A
• TNFalpha
• TNF-alpha
• TNF-alphacachectin
• TNFATNF, macrophage-derived
• TNFG1F
• TNFSF1A
• TNFSF2
• TNFSF2TNF superfamily, member 2
• tumor necrosis factor (TNF superfamily, member 2)
• tumor necrosis factor alpha
• Tumor necrosis factor ligand superfamily member 2
• tumor necrosis factor
• tumor necrosis factor-alpha

Background

Tumor necrosis factor (TNF)-alpha is a pro-inflammatory cytokine belonging to the TNF superfamily that is secreted by monocytes/macrophages, T cell, and natural killer (NK), among others (1). TNF-alpha is synthesized as a 233 amino acid (aa) transmembrane protein (mTNF-alpha) with a theoretical molecular weight (MW) of 26 kDa (1,2) that forms a homotrimer. mTNF-alpha is cleaved by TNF-alpha converting enzyme (TACE) and released in its 157 aa, 17 kDa soluble form (sTNF-alpha) (1-5). Both mTNF-alpha and sTNF-alpha are capable of binding type 1 TNF receptors (TNFR1), whereas mTNF-alpha predominately binds to TNFR2 (1,2). TNF-alpha binding to its receptors causes receptor recruitment of adaptor proteins, formation of signaling complexes, and downstream signaling cascades (e.g. MAPK, NF-kappaB, and Caspase-8), leading to distinct cellular responses such as survival, proliferation, inflammation, necroptosis, and apoptosis (1-5).

TNF-alpha is critical for normal immune response; however, dysregulation of TNF-alpha production can result in various pathologies (2,4,5). Excessive production of pro-inflammatory cytokines including interleukin 1 (IL-1), IL-6, and TNF-alpha has been implicated in an array of autoimmune diseases like rheumatoid arthritis (RA), inflammatory bowel disease (IBD), and psoriasis (2,4,5). Anti-TNF monoclonal antibodies, including Infliximab, and soluble TNFR have been approved for the treatment of autoimmune and TNF-mediated diseases (5). Additionally, data suggests that TNF inhibitors can be beneficial for treating patients experiencing immune-related adverse events associated with immune checkpoint inhibitor cancer treatment (6).

References

1. Holbrook J, Lara-Reyna S, Jarosz-Griffiths H, McDermott M. Tumour necrosis factor signalling in health and disease. F1000Res. 2019;8:F1000 Faculty Rev-111. https://doi.org/10.12688/f1000research.17023.1

2. Jang DI, Lee AH, Shin HY, et al. The Role of Tumor Necrosis Factor Alpha (TNF-alpha) in Autoimmune Disease and Current TNF-alpha Inhibitors in Therapeutics. Int J Mol Sci. 2021;22(5):2719. https://doi.org/10.3390/ijms22052719

3. Horiuchi T, Mitoma H, Harashima S, Tsukamoto H, Shimoda T. Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology (Oxford). 2010;49(7):1215-1228. https://doi.org/10.1093/rheumatology/keq031

4. Webster JD, Vucic D. The Balance of TNF Mediated Pathways Regulates Inflammatory Cell Death Signaling in Healthy and Diseased Tissues. Front Cell Dev Biol. 2020;8:365. https://doi.org/10.3389/fcell.2020.00365

5. Kalliolias GD, Ivashkiv LB. TNF biology, pathogenic mechanisms and emerging therapeutic strategies. Nat Rev Rheumatol. 2016; 12(1):49-62. https://doi.org/10.1038/nrrheum.2015.169

6. Chen AY, Wolchok JD, Bass AR. TNF in the era of immune checkpoint inhibitors: friend or foe?. Nat Rev Rheumatol. 2021;17(4):213-223. doi:10.1038/s41584-021-00584-4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF-alpha Antibody (NBP1-19532)(70)

Publications using NBP1-19532

• Abdelsalam M, El-Mahdy N, Abou-Saif S Direct-acting antivirals sofosbuvir and daclatasvir attenuate CCl4-induced liver fibrosis in mice Liver Research 2023-02-01 (IHC, Mouse)
• Wei F, Tuong ZK, Omer M et al. A novel multifunctional radioprotective strategy using P7C3 as a countermeasure against ionizing radiation-induced bone loss Bone research 2023-06-29 [PMID: 37385982] (IHC-Fr, Rat)
• Bouamar H, Broome L, Lathrop K et al. mTOR Inhibition Abrogates Human Mammary Stem Cells and Early Breast Cancer Progression Markers SSRN Electronic Journal 2022-07-10 (FLOW, Human)
• Pasupulati A, Nishad R, Mukhi D et al. Growth hormone induces TNF-alpha in podocytes and contributes to monocyte-to-macrophage differentiation: Implications in Diabetic kidney disease Research Square 2022-02-02
• Rayilla R, Phanithi P Traumatic Brain Injury: Crosstalk Between ER and Mitochondria Contributes to Oligodendrocyte Cell Death and Demyelination Research Square 2021-12-06 (IHC-P, Rat)
• Vos S, Aaron R, Weng M et al. CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression Investigative ophthalmology & visual science 2023-06-01 [PMID: 37294707] (ICC/IF, Human)
• Alam A, Singh T, Kayhanian S et al. Modelling the inflammatory response of traumatic brain injury using human induced pluripotent stem cell derived microglia Journal of neurotrauma 2023-06-01 [PMID: 37261979]
• Aswani SS, Mohan MS, Aparna NS et al. Oxidized LDL-mediated upregulation of ADAMTS-4 in monocytes/macrophages involves ROS-NF-?B-SIRT-1 pathway Physiology international 2023-05-22 [PMID: 37216221]
• Pérez-Acuña D, Rhee KH, Shin SJ et al. Retina-to-brain spreading of ?-synuclein after intravitreal injection of preformed fibrils Acta neuropathologica communications 2023-05-20 [PMID: 37210559] (IHC, Mouse)
• Abulfadl YS, El Ela YA, Al Khaiyat AM et al. Cyclophosphamide enfeebles myocardial isometric contraction force via RIP1/RIP3/MLKL/TRPM7-mediated necroptosis Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-07-01 [PMID: 37146416] (IHC-P, Rat)
• Nishad R, Mukhi D, Kethavath S et al. Podocyte derived TNF-alpha mediates monocyte differentiation and contributes to glomerular injury FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-12-01 [PMID: 36421039]
• Lindfors S Molecular mechanisms of podocyte injury: focus on adiponectin signalling in obesity and type 2 diabetes Thesis 2023-01-01 (IHC)
• Guo X, Sun Y, Wang Z et al. The Preventive Effect of A Magnetic Nanoparticle-Modified Root Canal Sealer on Persistent Apical Periodontitis International journal of molecular sciences 2022-10-28 [PMID: 36361925] (IHC-P, Canine)
  
  Details:
  Dilution used in IHC-P 1:300
• Khedr LH, Eladawy RM, Nassar NN, Saad MAE Canagliflozin attenuates chronic unpredictable mild stress induced neuroinflammation via modulating AMPK/mTOR autophagic signaling Neuropharmacology 2022-10-19 [PMID: 36272443] (IHC-P, Rat)
  
  Details:
  Dilution used in IHC 1:100
• Kochumon S, Hasan A, Al-Rashed F et al. Increased Adipose Tissue Expression of IL-23 Associates with Inflammatory Markers in People with High LDL Cholesterol Cells 2022-09-29 [PMID: 36231033] (IHC-P, Human)
  
  Details:
  Dilution used in IHC 1:200
• Hu Q, Yao J, Wu X Et al. Emodin attenuates severe acute pancreatitis-associated acute lung injury by suppressing pancreatic exosome-mediated alveolar macrophage activation Acta Pharmaceutica Sinica B 2021-10-01 [PMID: 36213542] (IHC-P, Rat)
• Dafnis I, Mountaki C, Fanarioti E et al. Temporal Pattern of Neuroinflammation Associated with a Low Glycemic Index Diet in the 5xFAD Mouse Model of Alzheimer's Disease Molecular neurobiology 2022-12-01 [PMID: 36175825] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC 1:200
• Chen K, Deng Y, Shang S et al. Complement factor B inhibitor LNP023 improves lupus nephritis in MRL/lpr mice Biomedicine & Pharmacotherapy 2022-09-01 [PMID: 36076550] (WB, Mouse)
• Ibrahim Y, Hammady S, Rifaai R et al. Dose-dependent ameliorating effect of lipoxin A4 on gentamicin-induced nephrotoxicity in rats: The role of TNF alpha, TGF- beta, ICAM-1, and JNK signaling Chemico-Biological Interactions 2022-08-01 [PMID: 36057361] (IHC-P, Rat)
  
  Details:
  IHC-P 1:100
• Perdigoto AL, Deng S, Du KC et al. Immune cells and their inflammatory mediators modify beta cells and cause checkpoint inhibitor-induced diabetes JCI insight 2022-08-04 [PMID: 35925682] (IHC-P, Mouse, Human)
• Chen H, Zhou J, Chen H et al. Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4 Clinical and translational medicine 2022-04-01 [PMID: 35390228] (IHC-P, Mouse)
• Zhang Y, Zhao M, Gao H et al. MAPK signalling-induced phosphorylation and subcellular translocation of PDHE1alpha promotes tumour immune evasion Nature metabolism 2022-03-01 [PMID: 35315437]
• Motawea A, Abd El Hady WE, Ahmed El-Emam G The protective impact of adapted trimebutine maleate-loaded nanostructured lipid carriers for alleviating the severity of acute colitis Drug delivery 2022-12-01 [PMID: 35297699]
• Narouiepour A, Ebrahimzadeh-Bideskan A, Rajabzadeh G et al. Neural stem cell therapy in conjunction with curcumin loaded in niosomal nanoparticles enhanced recovery from traumatic brain injury Scientific reports 2022-03-04 [PMID: 35246564] (IHC-P, Rat)
• Alzahrani B, Gaballa MMS, Tantawy AA et al. Blocking Toll-like receptor 9 attenuates bleomycin-induced pulmonary injury Journal of pathology and translational medicine 2022-03-01 [PMID: 35220710] (IF/IHC)
• Tanyeli A, Guzel Erdogan D, Comakli S et al. Therapeutic effects of apocynin on ovarian ischemia-reperfusion induced lung injury Biotechnic & histochemistry : official publication of the Biological Stain Commission 2022-02-14 [PMID: 35152781] (IF/IHC)
• Othman MA, Mubarak HA, Sayed MM Ameliorative role of alpha-lipoic acid in renal cortical structural damage, induced by limb ischemia-reperfusion injury in the rat Ultrastructural pathology 2022-02-08 [PMID: 35135431]
• Zhou J, Hou C, Chen H Et al. P16 I NK 4a Deletion Ameliorates Damage of Intestinal Epithelial Barrier and Microbial Dysbiosis in a Stress-Induced Premature Senescence Model of Bmi-1 Deficiency Frontiers in cell and developmental biology 2021-10-07 [PMID: 34712655] (ICC/IF, WB, Mouse)
• Guler Mc, Tanyeli A, Erdogan Dg Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation. Iran J Basic Med Sci 2021-10-29 [PMID: 34712424]
• Cheng YC, Chiu YM, Dai ZK, Wu BN Loganin Ameliorates Painful Diabetic Neuropathy by Modulating Oxidative Stress, Inflammation and Insulin Sensitivity in Streptozotocin-Nicotinamide-Induced Diabetic Rats Cells 2021-10-08 [PMID: 34685668] (Rat)
• Vendidandala NR, Yin TP, Nelli G Et al. Gallocatechin silver nanoparticle impregnated cotton gauze patches enhance wound healing in diabetic rats by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-kappa B pathways Life sciences 2021-10-06 [PMID: 34624322] (IHC-P, Rat)
• Velatooru LR, Abe RJ, Imanishi M Et al. Disturbed flow-induced FAK K152 SUMOylation initiates the formation of pro-inflammation positive feedback loop by inducing reactive oxygen species production in endothelial cells Free radical biology & medicine 2021-10-04 [PMID: 34619327]
• Choudhary V, Kaddour-Djebbar I, Custer VE Et al. Glycerol Improves Skin Lesion Development in the Imiquimod Mouse Model of Psoriasis: Experimental Confirmation of Anecdotal Reports from Patients with Psoriasis International journal of molecular sciences 2021-08-15 [PMID: 34445455] (IHC-P, Mouse)
• Yan Y, Liang H, Liu X Et Al. Topical cationic hairy particles targeting cell free DNA in dermis enhance treatment of psoriasis Biomaterials 2021-07-15 [PMID: 34293700]
• Wang M, Feng L, Li Z et al. Thymosin beta-4 reverses phenotypic polarization of glial cells and cognitive impairment via negative regulation of NF-kappa B signaling axis in APP/PS1 mice Journal of Neuroinflammation 2021-12-01 [PMID: 34183019] (IF/IHC, Mouse)
• Zheng C, Sui B, Zhang X et al. Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes Journal of Extracellular Vesicles 2021-05-01 [PMID: 34084287] (ICC/IF, Mouse)
• Iannucci J, Sen A, Grammas P Isoform-Specific Effects of Apolipoprotein E on Markers of Inflammation and Toxicity in Brain Glia and Neuronal Cells In Vitro Current issues in molecular biology 2021-05-27 [PMID: 34071762] (WB, Human)
• Lliberos C, Liew SH, Zareie P, et al. Evaluation of inflammation and follicle depletion during ovarian ageing in mice Scientific reports 2021-01-11 [PMID: 33432051] (WB, Mouse)
• Wu J, Dou Y, Ladiges W Adverse neurological effects of short-term sleep deprivation in aging mice are prevented by SS31 peptide Clocks Sleep 2020-10-22 [PMID: 33089207]
• Naranjo JD, Saldin LT, Sobieski E et al. Esophageal extracellular matrix hydrogel mitigates metaplastic change in a dog model of Barrett's esophagus Sci Adv 2020-07-01 [PMID: 32656339] (WB, Canine)
• Han Y, Lee Y, Jang Y et Al. Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARdelta-AMPK-PGC-1 alpha Pathway in Dyslipidemic Conditions BioMed Research International 2020-03-19 [PMID: 32258142] (WB, Mouse, Human)
• Wang M, Zong Hf, Chang Kw et Al. 5-HT1AR alleviates A beta-induced cognitive decline and neuroinflammation through crosstalk with NF-kappa B pathway in mice Int. Immunopharmacol. 2020-01-01 [PMID: 32143008] (IF/IHC, Mouse)
• Fu XQ, Peng J, Wang AH, Luo ZG Tumor necrosis factor alpha mediates neuromuscular synapse elimination Cell Discov 2020-03-03 [PMID: 32140252] (IF/IHC, Mouse)
• Li S, Jiang L, Yang Y et al. Siglec1 enhances inflammation through miR-1260-dependent degradation of I kappa B alpha in COPD Exp. Mol. Pathol. 2020-01-30 [PMID: 32007531] (WB, Rat)
• Wang M, Law ME, Davis BJ, et al. Identification of hub genes and key pathways of dietary advanced glycation end products induced non alcoholic fatty liver disease by bioinformatics analysis and animal experiments Mol Med Report 2019-12-09 [PMID: 31974594] (IF/IHC, Mouse)
• Li X, Xue YM, Guo HM et al. High hydrostatic pressure induces atrial electrical remodeling through upregulation of inflammatory cytokines Life Sci. 2019-12-20 [PMID: 31870776] (WB, Mouse, Rat)
• Sparks RP, Lui A, Bader D et al. A specific small-molecule inhibitor of Protein Kinase CdeltaI activity improves metabolic dysfunction in human adipocytes from obese individuals J. Biol. Chem. 2019-08-14 [PMID: 31413114] (WB, Human)
• Corbett BF, Luz S, Arner J et al. Sphingosine-1-phosphate receptor 3 in the medial prefrontal cortex promotes stress resilience by reducing inflammatory processes Nat Commun 2019-07-17 [PMID: 31316053] (IHC-Fr, Rat)
• Gu X, Peng CY, Lin SY et al. P16INK4a played a critical role in exacerbating acute tubular necrosis in acute kidney injury Am J Transl Res 2019-06-15 [PMID: 31312394] (WB, Mouse)
• Riggin, C N, Chen, M Et al. Ultrasound-Guided Dry Needling of the Healthy Rat Supraspinatus Tendon Elicits Early Healing Without Causing Permanent Damage. J Orthop Res 2019-09-01 [PMID: 31042318] (WB, Human)
• Bicakci, N;Karaboga, I;Dokmeci, AH;Guzel, S;Fidanol Erboga, Z; Cardioprotective effect of caffeic acid phenethyl ester on cardiac contusion following blunt chest trauma in rats Biotech Histochem 2019-04-01 [PMID: 30929510] (IF/IHC, Rat)
• Xing J, Lu J, Li J. Role of TNF-a in Regulating the Exercise Pressor Reflex in Rats With Femoral Artery Occlusion. Front Physiol. 2018-10-15 [PMID: 30374312] (ICC/IF, Rat)
• Park SH, Rhee J, Kim SK et al. BATF regulates collagen-induced arthritis by regulating T helper cell differentiation Arthritis Res. Ther. 2018-08-02 [PMID: 30071881] (IHC-P, ICC/IF, Mouse)
• Hoshikawa H, Kamitori K, Indo K, Mori T. Combined treatment with D-allose, docetaxel and radiation inhibits the tumor growth in anin vivomodel of head and neck cancer. Oncol Lett. 2018-03-01 [PMID: 29456721] (IF/IHC, Human)
• Ozdemira S, Altunb S, Arslan H. Imidacloprid exposure cause the histopathological changes, activation of TNF-a, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpio L.). Toxicology Reports 2017-01-01 [PMID: 29321977] (ICC/IF, Fish)
• Mohamed R, Coucha M, Elshaer SL et al. Inducible overexpression of endothelial proNGF as a mouse model to study microvascular dysfunction. Biochim. Biophys. Acta 2017-12-16 [PMID: 29253516] (Mouse)
• Huegel J, Choi DS, Nuss CA et al. Effects of pulsed electromagnetic field therapy at different frequencies and durations on rotator cuff tendon-to-bone healing in a rat model J Shoulder Elbow Surg 2017-11-22 [PMID: 29174271] (IF/IHC, Rat)
• Veruva SY, Lanman TH, Isaza JE et al. Periprosthetic UHMWPE Wear Debris Induces Inflammation, Vascularization, and Innervation After Total Disc Replacement in the Lumbar Spine. Clin. Orthop. Relat. Res. 2016-08-03 [PMID: 27488379] (IF/IHC, Mouse)
• Szylberg, Janiczek M, Popiel A, Marszatek A et al. Expression of COX-2, IL-1beta, TNF-alpha and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp Arch Med Sci 2016-02-01 [PMID: 26925134] (IHC-P, Human)
• Xu Y, Romero R, Miller D et al. An M1-like Macrophage Polarization in Decidual Tissue during Spontaneous Preterm Labor That Is Attenuated by Rosiglitazone Treatment J. Immunol. 2016-02-17 [PMID: 26889045] (IHC-Fr, FLOW, Human)
• Vieira Ramos G, Pinheiro CM, Messa SP et al. Cryotherapy Reduces Inflammatory Response Without Altering Muscle Regeneration Process and Extracellular Matrix Remodeling of Rat Muscle. Sci Rep. 2016-01-04 [PMID: 26725948] (IHC-Fr, Rat)
  
  Details:
  TNF alpha antibody (NBP1-19532) was used at 1:200 dilution for IHC-Fr staining of Tibialis anterior/TA muscle of rats 3, 7 and 14 days post-injury (subjected to freezing muscle injury and cryotherapy). TNF alpha expression was observed in CD68+ve cells and in injured muscle fibers.
• Acosta SA, Tajiri N, Hoover J et al. Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke. Stroke. 2015-09-01 [PMID: 26219646] (IHC-FrFl, Rat)
• Tucker JJ, Riggin CN, Connizzo BK et al. Effect of overuse-induced tendinopathy on tendon healing in a rat supraspinatus repair model J. Orthop. Res. 2015-07-27 [PMID: 26218457] (IHC-P, Rat)
• Castoldi G, di Gioia CR, Bombardi C et al. PREVENTION OF DIABETIC NEPHROPATHY BY COMPOUND 21, SELECTIVE AGONIST OF THE ANGIOTENSIN TYPE 2 RECEPTORS, IN ZUCKER DIABETIC FATTY RATS. Am. J Physiol. Renal Physiol. 2014-09-03 [PMID: 25186297]
• Thomas SJ, Sarver JJ, Yannascoli SM et al. Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model. J. Orthop. Res. 2014-07-28 [PMID: 25070655] (IHC-P, Rat)
  
  Details:
  TNF alpha/TNFa antibody used for IHC-P on whole shoulders of adult male Sprague-Dawley rats subjected to STZ induced hyperglycemia for 8 weeks - shoulders fixed in formalin, cut 7um longitudinal sections, peroxidase blocked in 3% H2O2/methanol 48C for 30 min, non-specific protein blocked with 4% dried milk in PBS /Blotto for 20 min, primary used at 1:750 dilution/PBS and incubated 4C ON followed by detection via goat anti-rabbit peroxidase-conjugated/HRP - DAB method. Immunostaining image not shown, but staining score on Supraspinatus tendon and Superior capsule tissues shown in Table 2.
• Piao HL, Yuan Y, Wang M et al. alpha-catenin acts as a tumour suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-kB signalling. Nat. Cell Biol. 2014-02-28 [PMID: 24509793] (IHC-P, Human)
• Chen X, Shi X, Zhang X et al. Scutellarin attenuates hypertension-induced expression of brain toll-like receptor 4/nuclear factor kappa B. Mediators Inflamm. 2013-01-01 [PMID: 24223475] (WB, Rat)
• Kwon KY, Jung HR, Hwang I, Choi WI. Evaluation of Bronchiolar and Alveolar Cell Injuries Induced by Short- and Long-term Exposure to Sidestream Smoke. Korean J Pathol 2012-04-01 [PMID: 23109995]

Reviews for TNF-alpha Antibody (NBP1-19532) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Rat, Other.

reviewed by: Nicholas Izzo ICC/IF Rat 10/13/2016

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Sample Tested: Primary neurons
• Species: Rat

reviewed by: Anonymous - IF Other 01/07/2016

Summary

• Application: Immunofluorescence
• Sample Tested: trabecular meshwork (TM) region of pig eyes
• Species: Other

reviewed by: Verified Customer WB Human 07/24/2014

Summary

• Application: Western Blot
• Sample Tested: Recombinant his-tagged TNFa made in E. coli cells
• Species: Human

FAQs for TNF-alpha Antibody (NBP1-19532). (Showing 1 - 7 of 7 FAQs).

1. I need to know about TNF-alpha antibody kits for mice.
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
5. We ordered a TNF alpha antibody from you back in May, and I am inquiring about the expiration date. The antibody we ordered was cat #NBP1-19532 and lot # 360986. . The data sheet says that short term storage at 4C and -20C for long term storage but that they are only guaranteed for 6 months. My question is are you considering the "short term storage" at 4C to be the 6 month expiration and -20C the antibody should still be OK? Typically we give our antibodies that are stored at -20C a two year expiration if one is not given.
   • We suggest to store the antibody at 4 degrees for no longer than a week. After this the product should be stored at -20 degrees and we are not sure if this is can be used after the 6 month period. It is likely that the antibody should be fine however we only guarantee our products for up to 6 months.
6. Is there any positive control tissue besides human colon carcinoma tissue that can be used for for paraffin embedded IHC testing?
   • You can use skin, lung, liver or mammary tumor tissue etc as a positive control for your assay.
7. I would like to learn about the TNF alpha antibody (NBP1-19532) immunohistochemistry protocol. Is there any specific protocol you have?
   • Our lab did not use any customized protocol for the IHC-P validation of this antibody. Please see our general IHC-P protocol.

Bioinformatics

• Gene Symbol: TNF
• Entrez:
  • 7124(Human)
  • 21926(Mouse)
  • 24835(Rat)
• Uniprot:
  • P01375()