Recombinant Human Lymphotoxin-alpha/TNF-beta Protein, CF

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1 µg/lane of Recombinant Human Lymphotoxin-alpha /TNF-beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 19 kDa.
Recombinant Human Lymphotoxin-alpha /TNF-beta (Catalog # 211-TBB/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Lymphotoxin-alpha/TNF-beta Protein, CF Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-20 pg/mL.
Source
E. coli-derived human Lymphotoxin-alpha/TNF-beta protein
Leu35-Leu205, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
LTA
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17-19 kDa, reducing conditions
Publications
Read Publications using
211-TBB/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein, CF

  • LT
  • LTA
  • LT-alpha
  • lymphotoxin alpha (TNF superfamily, member 1)
  • Lymphotoxin alpha
  • Lymphotoxinalpha
  • Lymphotoxin-alpha
  • tnfb
  • TNF-beta
  • TNFBlymphotoxin-alpha
  • TNFSF1
  • TNFSF1B
  • TNFSF1TNF-beta
  • tumor necrosis factor beta
  • Tumor necrosis factor ligand superfamily member 1

Background

Lymphotoxin-alpha (LT-alpha ), also known as Tumor Necrosis Factor-beta (TNF-beta ), is a member of the TNF Superfamily. Human LT-alpha /TNF-beta is a 22 kDa protein that shares 73% amino acid sequence identity with mouse and rat LT-alpha /TNF-beta (1-3). Secreted LT-alpha forms homotrimers that bind and activate TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, and HVEM/TNFRSF14 (4). LT-alpha /TNF-beta also forms heterotrimers with plasma membrane-localized LT-beta to bind and activate the LT-beta R/TNFRSF3 (4). In addition to its cytotoxic action on tumor cells, LT-alpha /TNF-beta mediates lymph node development, inflammation, and immune function (5-8). LT-alpha /TNF-beta is expressed in activated T- and B lymphocytes and contributes to autoimmune disease (9, 10).

  1. Nedwin, G.E. et al. (1985) J. Cell. Biochem. 29:171.
  2. Gray, P.W. et al. (1984) Nature 312:721.
  3. Kobayashi, Y. et al. (1986) J. Biochem. 100:727.
  4. Warzocha, K. et al. (1995) Eur. Cytokine Netw. 6:83.
  5. McCarthy, D.D. et al.(2006) Immunol. Res. 35:41.
  6. Drutskaya, M.S. et al. (2010) IUBMB Life 62:283.
  7. De Togni, P. et al. (1994) Science 264:703.
  8. Seleznik, G.M. et al. (2014) Cytokine Growth Factor Rev. 25:125.
  9. Mapara, M.Y. et al. (1994) Int. J. Cancer 58:248.
  10. Chiang, E.Y. et al. (2009) Nat. Med. 15:766.

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211-TBB/CF
Species: Hu
Applications: Bioactivity

Publications for Lymphotoxin-alpha/TNF-beta (211-TBB/CF)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


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Bioassay
(5)
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Human
(3)
Mouse
(2)
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Showing Publications 1 - 5 of 5.
Publications using 211-TBB/CF Applications Species
JG Chen, X Liu, M Munshi, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, GG Chan, CJ Patterson, KE Meid, J Gustine, T Dubeau, P Severns, JJ Castillo, ZR Hunter, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism Blood, 2018;0(0):. 2018 [PMID: 29496671] (Bioassay, Human) Bioassay Human
Sarhan D, Palma M, Mao Y, Adamson L, Kiessling R, Mellstedt H, Osterborg A, Lundqvist A Dendritic cell regulation of NK-cell responses involves lymphotoxin-alpha, IL-12, and TGF-beta. Eur J Immunol, 2015;45(6):1783-93. 2015 [PMID: 25773885] (Bioassay, Human) Bioassay Human
Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1. J Biol Chem, 2015;290(7):4022-37. 2015 [PMID: 25538244] (Bioassay, Human) Bioassay Human
Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice. Am J Pathol, 2014;184(4):1152-66. 2014 [PMID: 24655377] (Bioassay, Mouse) Bioassay Mouse
Vang, Amanda G, Housley, William, Dong, Hongli, Basole, Chaitali, Ben-Sasson, Shlomo Z, Kream, Barbara, Epstein, Paul M, Clark, Robert B, Brocke, Stefan Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac. Biochem J, 2013;456(3):463-73. 2013 [PMID: 24007532] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol LTA
Uniprot