TLR4 Antibody (HTA1245)

Images

 
Flow Cytometry: TLR4 Antibody (HTA1245) [NBP1-51697] - Analysis using FITC conjugate of NBP1-51697. Staining of cells transfected with human TLR4 with Mouse IgG2a K Isotype Control PE-Cy7 (open histogram) or Anti-Human ...read more
Flow Cytometry: TLR4 Antibody (HTA1245) [NBP1-51697] - Analysis using FITC conjugate of NBP1-51697. Cell surface Analysis of TLR4 in 10^6 human PBMCs using 0.5 ugs of this antibody. The shaded histogram represents cells ...read more
Flow Cytometry: TLR4 Antibody (HTA1245) [NBP1-51697] - Analysis using FITC conjugate of NBP1-51697. Cell surface flow Analysis of TLR4 in stable HEK293/hTLR4 cells using this antibody at 1ug/10^6 cells. Both stable ...read more

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB, Flow, Func, IA, ICC/IF, IP
Clone
HTA1245
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

TLR4 Antibody (HTA1245) Summary

Immunogen
BALB/c mice were immunized with the Ba/F3 line expressing TLR4.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TLR4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
This product works in Flow Cytometry, Functional, Immunoassay, Immunofluorescence, Immunoprecipitation and Western Blot.
Agonist
Antagonist

Reactivity Notes

Reats with human. Cross reatcs with Canine, Cynomolgus monkey, Rhesus monkey and Marmoset monkey.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
No Preservative
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Notes

Positive Control: Macrophages Negative Control - HEK293 cells

Alternate Names for TLR4 Antibody (HTA1245)

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFKBdependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single a-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Toll-like receptor 4 (TLR4; CD284) has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways; one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of pro-inflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIRdomaincontaining adaptor protein inducing interferon-B) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. The monoclonal antibody HTA125 is a TLR4 function-blocking antibody. HTA125 recognizes preferentially human TLR4 that is associated with MD-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TLR4 Antibody (NBP1-51697)

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Blogs on TLR4.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

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Bioinformatics

Gene Symbol TLR4
Entrez