TLR4 Antibody (1203B) [Unconjugated]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

TLR4 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: Flow, ICC/IF
• Clone: 1203B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

TLR4 Antibody (1203B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TLR4
  Asn26-Lys629
  Accession # Q9QUK6
• Specificity: Detects mouse TLR4 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Tlr4
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLR4 Antibody (1203B) [Unconjugated]

• ARMD10
• CD_antigen: CD284
• CD284 antigen
• CD284
• EC 3.2.2.6
• EC:3.2.2.6
• homolog of Drosophila toll
• hToll
• TLR4
• TLR-4
• toll like receptor 4 protein
• TOLL
• toll-like receptor 4

Background

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR4 Antibody (MAB27591)(3)

Publications using MAB27591

• Yulia Kiyan, Sergey Tkachuk, Song Rong, Anna Gorrasi, Pia Ragno, Inna Dumler et al. TLR4 Response to LPS Is Reinforced by Urokinase Receptor Frontiers in Immunology 12/9/2020 [PMID: 33362762]
• D Bonhomme, I Santecchia, F Vernel-Pau, M Caroff, P Germon, G Murray, B Adler, IG Boneca, C Werts Leptospiral LPS escapes mouse TLR4 internalization and TRIF?associated antimicrobial responses through O antigen and associated lipoproteins PLoS Pathog., 2020-08-13;16(8):e1008639. 2020-08-13 [PMID: 32790743] (Flow Cytometry, ICC, Mouse)
• Andrew M. Breglio, Lindsey A. May, Melanie Barzik, Nora C. Welsh, Shimon P. Francis, Tucker Q. Costain et al. Exosomes mediate sensory hair cell protection in the inner ear Journal of Clinical Investigation 4/20/2020 [PMID: 32027617]

Bioinformatics

• Gene Symbol: Tlr4
• Uniprot:
  • Q9QUK6()