Rabbit IgG Isotype Control [Unconjugated]

HepG2 humanhepatocellular carcinoma cell line was stained with Rabbit Anti-HumanKlotho beta Monoclonal Antibody (Catalog # MAB58891,filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # AB-105-C,open histogram), followed byAllophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #F0111). View our protocol for StainingMembrane-associated Proteins.

• Reactivity: Rb
• Applications: Ctrl
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Rabbit IgG Isotype Control [Unconjugated] Summary

• Immunogen: NA
• Specificity: Serum was obtained from naive (non-immunized) rabbits and purified for use as normal rabbit IgG.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHG1

Applications/Dilutions

Reviewed Applications

Read 4 Reviews rated 5 using AB-105-C in the following application: Immunocytochemistry/Immunofluorescence Serum

Publications

Read Publications using AB-105-C in the following applications: ChIP 4 publications ChIP Control 2 publications Co-Immunoprecipitation 1 publication Control 11 publications Flow Cytometry 2 publications Flow Cytometry Control 1 publication ICC Control 1 publication IHC 5 publications IHC Control 2 publications IHC-Fr 3 publications IHC-P 3 publications Immunoprecipitation 3 publications In Vivo 1 publication Isotype Control 2 publications Neutralization 5 publications Neutralization Control 2 publications Western Blot 3 publications

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Dissolve the lyophilized rabbit IgG in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rabbit IgG Isotype Control [Unconjugated]

• IgG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (AB-105-C)(51)

Publications using AB-105-C

• L Ji, T Wang, L Tian, H Song, M Gao Roxatidine inhibits fibrosis by inhibiting NF?kappaB and MAPK signaling in macrophages sensing breast implant surface materials Mol Med Rep, 2019;0(0):. 2019 [PMID: 31746427] (Neutralization, Mouse)
• M Murata, K Noda, S Yoshida, M Saito, A Fujiya, A Kanda, S Ishida Unsaturated Aldehyde Acrolein Promotes Retinal Glial Cell Migration Invest. Ophthalmol. Vis. Sci., 2019;60(13):4425-4435. 2019 [PMID: 31652327] (IHC-P, Human)
• A Linder, M Hagberg Th, JE Damber, K Welén Analysis of regulator of G-protein signalling 2 (RGS2) expression and function during prostate cancer progression Sci Rep, 2018;8(1):17259. 2018 [PMID: 30467386] (IHC-P, Human)
• S Chen, TR Luperchio, X Wong, EB Doan, AT Byrd, K Roy Choudh, KL Reddy, MS Krangel A Lamina-Associated Domain Border Governs Nuclear Lamina Interactions, Transcription, and Recombination of the Tcrb Locus Cell Rep, 2018;25(7):1729-1740.e6. 2018 [PMID: 30428344] (ChIP Control, Mouse)
• J Xia, L Kong, LJ Zhou, SZ Wu, LJ Yao, C He, CY He, HJ Peng Genome-Wide Bimolecular Fluorescence Complementation-Based Proteomic Analysis ofToxoplasma gondiiROP18's Human Interactome Shows Its Key Role in Regulation of Cell Immunity and Apoptosis Front Immunol, 2018;9(0):61. 2018 [PMID: 29459857] (Western Blot, Primate - Chlorocebus aethiops (African Green Monkey))
• GA Howe, K Kazda, CL Addison MicroRNA-30b controls endothelial cell capillary morphogenesis through regulation of transforming growth factor beta 2 PLoS ONE, 2017;12(10):e0185619. 2017 [PMID: 28977001] (Western Blot, Human)
• SS Bakke, MH Aune, N Niyonzima, K Pilely, L Ryan, M Skjelland, P Garred, P Aukrust, B Halvorsen, E Latz, JK Damås, TE Mollnes, T Espevik Cyclodextrin Reduces Cholesterol Crystal-Induced Inflammation by Modulating Complement Activation J. Immunol., 2017;0(0):. 2017 [PMID: 28855312] (Flow Cytometry, Human)
• L Kumar Dube, P Karempudi, SA Luther, B Ludewig, NL Harris Interactions between fibroblastic reticular cells and B cells promote mesenteric lymph node lymphangiogenesis Nat Commun, 2017;8(1):367. 2017 [PMID: 28848229] (Flow Cytometry, Mouse)
• M Yang, CJ Li, X Sun, Q Guo, Y Xiao, T Su, ML Tu, H Peng, Q Lu, Q Liu, HB He, TJ Jiang, MX Lei, M Wan, X Cao, XH Luo MiR-497?195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1? activity Nat Commun, 2017;8(0):16003. 2017 [PMID: 28685750] (IHC, Mouse)
• SS Ganaie, W Zou, P Xu, X Deng, S Kleiboeker, J Qiu Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex PLoS Pathog., 2017;13(5):e1006370. 2017 [PMID: 28459842] (Western Blot, Human)
• L Zhu, A Gomez-Dura, G Saretzki, S Jin, K Tilgner, D Melguizo-S, G Anyfantis, J Al-Aama, L Vallier, P Chinnery, M Lako, L Armstrong The mitochondrial protein CHCHD2 primes the differentiation potential of human induced pluripotent stem cells to neuroectodermal lineages J. Cell Biol., 2016;215(2):187-202. 2016 [PMID: 27810911] (ChIP, Human)
• P Dmitriev, E Kiseleva, O Kharchenko, E Ivashkin, A Pichugin, P Dessen, T Robert, F Coppée, A Belayew, G Carnac, D Laoudj-Che, M Lipinski, A Vasiliev, YS Vassetzky Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis Oncotarget, 2016;7(40):65090-65108. 2016 [PMID: 27556182] (Control, Human)
• X Chen, DL Rateri, DA Howatt, A Balakrishn, JJ Moorleghen, LA Cassis, A Daugherty TGF-? Neutralization Enhances AngII-Induced Aortic Rupture and Aneurysm in Both Thoracic and Abdominal Regions PLoS ONE, 2016;11(4):e0153811. 2016 [PMID: 27104863] (Neutralization, Mouse)
• SY Lim, AE Yuzhalin, AN Gordon-Wee, RJ Muschel Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells Oncogene, 2016;0(0):. 2016 [PMID: 27086923] (IHC-Fr, Mouse)
• A Spiegel, MW Brooks, S Houshyar, F Reinhardt, M Ardolino, E Fessler, MB Chen, JA Krall, J DeCock, IK Zervantona, A Iannello, Y Iwamoto, V Cortez-Ret, RD Kamm, MJ Pittet, DH Raulet, RA Weinberg Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells Cancer Discov, 2016;0(0):. 2016 [PMID: 27072748] (In Vivo, Mouse)
• Morrison G, Scognamiglio R, Trumpp A, Smith A Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification. EMBO J, 2015;35(3):356-68. 2015 [PMID: 26675138] (ChIP, Mouse)
• Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha. PLoS ONE, 2015;10(9):e0137612. 2015 [PMID: 26367137] (IHC Control, Human)
• Au L, Meisch J, Das L, Binko A, Boxer R, Wen A, Steinmetz N, Lu K Suppression of Hyperactive Immune Responses Protects against Nitrogen Mustard Injury. J Invest Dermatol, 2015;135(12):2971-81. 2015 [PMID: 26288355] (IHC, Mouse)
• Chen L, Carico Z, Shih H, Krangel M A discrete chromatin loop in the mouse Tcra-Tcrd locus shapes the TCRdelta and TCRalpha repertoires. Nat Immunol, 2015;16(10):1085-93. 2015 [PMID: 26258942] (ChIP, Mouse)
• Spronken M, Short K, Herfst S, Bestebroer T, Vaes V, van der Hoeven B, Koster A, Kremers G, Scott D, Gultyaev A, Sorell E, de Graaf M, Barcena M, Rimmelzwaan G, Fouchier R Optimisations and Challenges Involved in the Creation of Various Bioluminescent and Fluorescent Influenza A Virus Strains for In Vitro and In Vivo Applications. PLoS ONE, 2015;10(8):e0133888. 2015 [PMID: 26241861] (IHC Control, Mouse)
• Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes. J Neuroinflammation, 2015;12(0):119. 2015 [PMID: 26077779] (IHC, Human)
• Angelucci C, Maulucci G, Colabianchi A, Iacopino F, D'Alessio A, Maiorana A, Palmieri V, Papi M, De Spirito M, Di Leone A, Masetti R, Sica G Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts. Br J Cancer, 2015;112(10):1675-86. 2015 [PMID: 25880005] (Neutralization Control, Human)
• Hao B, Naik A, Watanabe A, Tanaka H, Chen L, Richards H, Kondo M, Taniuchi I, Kohwi Y, Kohwi-Shigematsu T, Krangel M An anti-silencer- and SATB1-dependent chromatin hub regulates Rag1 and Rag2 gene expression during thymocyte development. J Exp Med, 2015;212(5):809-24. 2015 [PMID: 25847946] (Immunoprecipitation, Mouse)
• del Blanco B, Angulo U, Krangel M, Hernandez-Munain C T-cell receptor alpha enhancer is inactivated in alphabeta T lymphocytes. Proc Natl Acad Sci U S A, 2015;112(14):E1744-53. 2015 [PMID: 25831496] (Immunoprecipitation, Mouse)
• Yoshihara M, Ohmiya H, Hara S, Kawasaki S, Hayashizaki Y, Itoh M, Kawaji H, Tsujikawa M, Nishida K Discovery of molecular markers to discriminate corneal endothelial cells in the human body. PLoS ONE, 2015;10(3):e0117581. 2015 [PMID: 25807145] (IHC, Human)
• Yoon, Se-Jin, Foley, Joseph W, Baker, Julie C HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun, 2015;6(0):6546. 2015 [PMID: 25775035] (Co-Immunoprecipitation, Mouse)
• Gonzalez-Valdes I, Hidalgo I, Bujarrabal A, Lara-Pezzi E, Padron-Barthe L, Garcia-Pavia P, Gomez-del Arco P, Redondo J, Ruiz-Cabello J, Jimenez-Borreguero L, Enriquez J, de la Pompa J, Hidalgo A, Gonzalez S Bmi1 limits dilated cardiomyopathy and heart failure by inhibiting cardiac senescence. Nat Commun, 2015;6(0):6473. 2015 [PMID: 25751743] (Neutralization, Mouse)
• Li, Chang-Ju, Cheng, Peng, Liang, Meng-Ke, Chen, Yu-Si, Lu, Qiong, Wang, Jin-Yu, Xia, Zhu-Ying, Zhou, Hou-De, Cao, Xu, Xie, Hui, Liao, Er-Yuan, Luo, Xiang-Ha MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation. J Clin Invest, 2015;125(4):1509-22. 2015 [PMID: 25751060] (IHC-P, Mouse)
• Ueki Y, Wilken M, Cox K, Chipman L, Bermingham-McDonogh O, Reh T A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation. Development, 2015;142(3):533-43. 2015 [PMID: 25605781] (ChIP, Mouse)
• Bernard M, Dieude M, Yang B, Hamelin K, Underwood K, Hebert M Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF. Autophagy, 2014;10(12):2193-207. 2014 [PMID: 25495560] (Neutralization, Human)
• Naik A, Hawwari A, Krangel M Specification of Vdelta and Valpha usage by Tcra/Tcrd locus V gene segment promoters. J Immunol, 2015;194(2):790-4. 2015 [PMID: 25472997] (ChIP Control, Mouse)
• Astarita, Jillian, Cremasco, Viviana, Fu, Jianxin, Darnell, Max C, Peck, James R, Nieves-Bonilla, Janice M, Song, Kai, Kondo, Yuji, Woodruff, Matthew, Gogineni, Alvin, Onder, Lucas, Ludewig, Burkhard, Weimer, Robby M, Carroll, Michael, Mooney, David J, Xia, Lijun, Turley, Shannon The CLEC-2-podoplanin axis controls the contractility of fibroblastic reticular cells and lymph node microarchitecture. Nat Immunol, 2015;16(1):75-84. 2015 [PMID: 25347465] (Control, Mouse)
• Hou X, Song J, Su J, Huang D, Gao W, Yan J, Shen J CD4(+)Foxp3(+) Tregs protect against innate immune cell-mediated fulminant hepatitis in mice. Mol Immunol, 2015;63(2):420-7. 2015 [PMID: 25315497] (Neutralization Control, Mouse)
• Tomellini E, Touil Y, Lagadec C, Julien S, Ostyn P, Ziental-Gelus N, Meignan S, Lengrand J, Adriaenssens E, Polakowska R, Le Bourhis X Nerve growth factor and proNGF simultaneously promote symmetric self-renewal, quiescence, and epithelial to mesenchymal transition to enlarge the breast cancer stem cell compartment. Stem Cells, 2015;33(2):342-53. 2015 [PMID: 25286822] (ICC Control, Human)
• De Simone V, Franze E, Ronchetti G, Colantoni A, Fantini M, Di Fusco D, Sica G, Sileri P, MacDonald T, Pallone F, Monteleone G, Stolfi C Th17-type cytokines, IL-6 and TNF-alpha synergistically activate STAT3 and NF-kB to promote colorectal cancer cell growth. Oncogene, 2015;34(27):3493-503. 2015 [PMID: 25174402] (IHC-Fr, Mouse)
• Gama J, Ohlmeier S, Martins T, Fraga A, Sampaio-Marques B, Carvalho M, Proenca F, Silva M, Pedrosa J, Ludovico P Proteomic analysis of the action of the Mycobacterium ulcerans toxin mycolactone: targeting host cells cytoskeleton and collagen. PLoS Negl Trop Dis, 2014;8(8):e3066. 2014 [PMID: 25101965] (Neutralization, Mouse)
• Fujishiro A, Kaneko H, Kawashima T, Ishida M, Kawano T In vivo neuronal action potential recordings via three-dimensional microscale needle-electrode arrays. Sci Rep, 2014;4(0):4868. 2014 [PMID: 24785307] (IHC-Fr, Rat)
• Morshed M, Hlushchuk R, Simon D, Walls A, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon H, Yousefi S NADPH oxidase-independent formation of extracellular DNA traps by basophils. J Immunol, 2014;192(11):5314-23. 2014 [PMID: 24771850] (Isotype Control, Mouse)
• Samstad E, Niyonzima N, Nymo S, Aune M, Ryan L, Bakke S, Lappegard K, Brekke O, Lambris J, Damas J, Latz E, Mollnes T, Espevik T Cholesterol crystals induce complement-dependent inflammasome activation and cytokine release. J Immunol, 2014;192(6):2837-45. 2014 [PMID: 24554772] (Isotype Control, Human)
• Houser J, Komarek J, Kostlanova N, Cioci G, Varrot A, Kerr S, Lahmann M, Balloy V, Fahy J, Chignard M, Imberty A, Wimmerova M A soluble fucose-specific lectin from Aspergillus fumigatus conidia--structure, specificity and possible role in fungal pathogenicity. PLoS ONE, 2013;8(12):e83077. 2013 [PMID: 24340081] (IHC, Fungus - Aspergillus fumigatus)
• Yu, Da-Hai, Ware, Carol, Waterland, Robert A, Zhang, Jiexin, Chen, Miao-Hsu, Gadkari, Manasi, Kunde-Ramamoorthy, Govindar, Nosavanh, Lagina M, Shen, Lanlan Developmentally programmed 3' CpG island methylation confers tissue- and cell-type-specific transcriptional activation. Mol Cell Biol, 2013;33(9):1845-58. 2013 [PMID: 23459939] (Immunoprecipitation, Human)
• Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation. Mucosal Immunol, 2012;5(0):702. 2012 [PMID: 22692452] (Control, Human)
• Yamakawa N, Uchida T, Matthay MA, Makita K Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25. 2011 [PMID: 21257730] (Control, Rat)
• Chauchereau A, Al Nakouzi N, Gaudin C, Le Moulec S, Compagno D, Auger N, Benard J, Opolon P, Rozet F, Validire P, Fromont G, Fizazi K Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer. Exp. Cell Res., 2011;317(3):262-75. 2011 [PMID: 20974126] (Flow Cytometry Control, Human)
• Suzuki K, Maruya M, Kawamoto S The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut. Immunity, 2010;33(1):71-83. 2010 [PMID: 20643338] (Control, Mouse)
• Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose. J. Clin. Oncol., 2007;25(29):4542-9. 2007 [PMID: 17925549] (Control, Human)
• Bolovan-Fritts CA, Spector SA Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction. Blood, 2007;111(1):175-82. 2007 [PMID: 17895402] (Control, Human)
• Matsumura S, Shibakusa T, Fujikawa T, Yamada H, Inoue K, Fushiki T Increase in transforming growth factor-beta in the brain during infection is related to fever, not depression of spontaneous motor activity. Neuroscience, 2007;144(3):1133-40. 2007 [PMID: 17156928] (Control, Rat)
• Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates. J. Immunol., 2004;172(9):5753-64. 2004 [PMID: 15100322] (Control, Primate - Macaca mulatta (Rhesus Macaque))
• Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10. J. Immunol., 2004;172(9):5213-21. 2004 [PMID: 15100259] (Control, Human)
• Viti J, Gulacsi A, Lillien L Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2. J. Neurosci., 2003;23(13):5919-27. 2003 [PMID: 12843296] (Control, Mouse)

Reviews for IgG Isotype Control (AB-105-C) (4)

Average Rating: 5

(Based on 4 reviews)

reviewed by: Anonymous ICC/IF 05/07/2019

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Lot: er1618101

reviewed by: Anonymous 05/07/2019

Summary

• Application: Serum
• Lot: er1618101

reviewed by: Anonymous ICC/IF 04/19/2018

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Lot: ER1517081

reviewed by: Anonymous 04/19/2018

Summary

• Application: Serum
• Lot: ER1517081

Bioinformatics

• Gene Symbol: IGHG1