TLR4 Antibody (1039412) [Unconjugated]

Western blot shows lysates of RT4 human bladder carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TLR4 Monoclonal Antibody (Catalog # MAB11187) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TLR4 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 1039412
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TLR4 Antibody (1039412) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived human TLR4
  Glu24-Lys631
  Accession # O00206
• Specificity: Detects human TLR4 in direct ELISAs
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLR4 Antibody (1039412) [Unconjugated]

• ARMD10
• CD_antigen: CD284
• CD284 antigen
• CD284
• EC 3.2.2.6
• EC:3.2.2.6
• homolog of Drosophila toll
• hToll
• TLR4
• TLR-4
• toll like receptor 4 protein
• TOLL
• toll-like receptor 4

Background

TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll‑Like Receptor family of pathogen pattern recognition molecules. The complex of TLR4 with MD‑2 functions as a critical receptor for bacterial endotoxin/lipopolysaccharide (LPS) (1-3). Mature human TLR4 consists of a 608 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 187 aa cytoplasmic domain. TLR4 contains 21 leucine rich repeats in its ECD and one cytoplasmic Toll/IL‑1 receptor (TIR) domain (4). The ECD of human TLR4 shares approximately 25% aa sequence identity with other human TLRs and 60%-74% aa sequence identity with bovine, equine, feline, mouse, rat, and porcine TLR4. On monocytes, macrophages, dendritic cells, and B cells, MD-2 expression is required for cell surface localization of TLR4 and for optimal LPS-induced TLR4 signaling (5-8). MD-2 also forms soluble disulfide‑linked homo‑oligomers which can interact with TLR4 (6). Through a domain separate from its TLR4-binding domain, MD-2 extracts LPS from circulating CD14-LPS complexes and carries the LPS into a ternary complex with TLR4 (9-11). The interaction of MD-2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (2, 12). Increased levels of plasma MD-2 in septic shock patients sensitizes MD-2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (13).

1. Miyake, K. (2004) Semin. Immunol. 16:11.
2. Verstrepen, L. et al. (2008) Cell. Mol. Life Sci. 65:2964.
3. Erridge, C. (2010) J. Leukoc. Biol. 87:989.
4. Medzhitov, R. et al. (1997) Nature 388:394.
5. Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
6. Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. 98:12156.
7. Akashi, S. et al. (2000) J. Immunol. 164:3471.
8. Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
9. Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
10. Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
11. Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. 101:4186.
12. Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
13. Pugin, J. et al. (2004) Blood 104:4071.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O00206()