TLR10 Antibody (3C10C5) [PE]

Flow Cytometry: TLR10 Antibody (3C10C5) [PE] [NBP2-27244] - Cell surface analysis using PE-conjugated TLR10 antibody. Human PBMC were probed using 10 ul (1 ug) of TLR10 antibody and 1 ug of CD19 antibody this antibody . This antibody was used for this test.

• Reactivity: Hu
• Applications: Flow
• Clone: 3C10C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

TLR10 Antibody (3C10C5) [PE] Summary

• Immunogen: A partial recombinant protein containing amino acids 20-474 (extracellular domain) of human TLR10.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR10
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1ul/1 million cells
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for TLR10 Antibody (3C10C5) [PE]

• CD290 antigen
• CD290
• MGC104967
• MGC126398
• MGC126399
• TLR10
• toll-like receptor 10

Background

Toll-like receptors (TLRs) are one of the mechanisms by which the innate immune system senses the invasion of pathogenic microorganisms (reviewed in 1). TLRs are primary transmembrane proteins of immune cells; they have an extracellular region which contains leucine-rich repeat motifs, and a cytoplasmic tail, which has a Toll/interleukin-1 (IL-1) receptor (TIR) domain. TLRs are pattern recognition receptors (PRRs ) that bind to pathogen-associated molecular patterns (PAMPs). Different TLRs recognize different surface and intracellular components of microorganisms or PAMPS. The interaction between a TLR and a microbial component or PAMP triggers the activation of the innate immune system, as well as the development of acquired immunity. Interaction of PAMPs with different TLRs induces distinct patters of gene expression through signal transduction pathways. TLR signaling occurs through the TIR domains which utilize adaptors such as MyD88, TIRAP, TRIF, and TRAM. NF-kapppa-B and JNk pathways are activated leading to the transcription of genes encoding pro-inflammatory cytokines. TLR-10, originally identified from a spleen cDNA library, is on chromosome 4p14 and has been found to be expressed in lung and in B-lymphocytes (2). TLR10 is thought to be a potential asthma candidate gene because early life innate immune responses to ubiquitous inhaled allergens and PAMPs may influence asthma susceptibility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLR10