Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free

Western Blot: Tissue alpha-L-Fucosidase/FUCA1 Antibody [NBP3-00209] - NBP3-00209 at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:54 kDa

Immunoprecipitation: Tissue alpha-L-Fucosidase/FUCA1 Antibody [NBP3-00209] - FUCA1 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 1 uL NBP3-00209 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00209, at 1:500 dilution. Secondary antibody: IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 54 kDa Observed band size: 54 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human Tissue alpha-L-Fucosidase/FUCA1 (Accession#: P04066; Gln32-Lys466)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FUCA1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunoprecipitation 1-2 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Tissue alpha-L-Fucosidase/FUCA1 Antibody - Azide and BSA Free

• Alpha-L-fucosidase 1
• Alpha-L-fucosidase I
• Alpha-L-fucoside fucohydrolase 1
• EC 3.2.1
• EC 3.2.1.51
• FUCA
• FUCA1
• fucosidase, alpha-L- 1, tissue
• Tissue alphaLFucosidase
• Tissue alpha-L-Fucosidase

Background

Alpha-L-fucosidase (EC 3.2.1.51) is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids (Occhiodoro et al., 1989 [PubMed 2803312]). At least 2 separate polymorphic alpha-L-fucosidases are recognized in man: that in tissues, FUCA1, which is deficient in fucosidosis (MIM 230000), and that in plasma, FUCA2 (MIM 136820).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUCA1