RHCE Antibody (BRIC69) - BSA Free

• Reactivity: Hu
• Applications: Flow, IP
• Clone: BRIC69
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RHCE Antibody (BRIC69) - BSA Free Summary

• Immunogen: Human erythrocytes
• Specificity: Recognizes human erythrocytes of normal Rh types but does not recognize Rh null erythrocytes. NB100-65887 reacts with 31kD and 35-52kD proteins which were designated CD240DCE at the 7th leucocyte typing workshop.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHCE
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine (pH 7.7)
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for RHCE Antibody (BRIC69) - BSA Free

• blood group Rh(CE) polypeptide
• blood group RhCcEe antigen
• CD240CE antigen
• CD240CE
• MGC103977
• Rh blood group antigen Evans
• Rh blood group C antigen
• Rh blood group, CcEe antigens
• Rh polypeptide 1
• Rh polypeptide I
• RH
• RH30A
• Rh4
• RHC
• RHCE blood group variant Crawford antigen Rh43
• RHE
• Rhesus blood group CE protein
• Rhesus blood group E antigen
• Rhesus blood group Rhce antigen
• Rhesus blood group, CcEe antigens
• Rhesus C/E antigens
• Rhesus system C and E polypeptides
• RhIVb(J)
• RHIXB
• RHPI
• RhVI
• RhVIII
• silenced Rh blood group CcEe antigen

Background

The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The classification of Rh positive and Rh negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Alternative splicing of the gene results in four transcript variants encoding four different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD240DCE Antibody (NB100-65887)(1)

Publication using NB100-65887

• Avent N, Judson PA, Parsons SF et al. Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific. Biochem J;251(2):499-505. 1988-04-15 [PMID: 3135800]

Bioinformatics

• Gene Symbol: RHCE
• Entrez:
  • 6006(Human)
• Uniprot:
  • P18577(Human)