RHD Antibody (D7C2) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: RHD Antibody (D7C2) - Chimeric [NBP3-09042] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of D7C2 (NBP3-09042) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09042, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: RHD Antibody (D7C2) - Chimeric - Azide and BSA Free [NBP3-09042] - Paraformaldehyde fixed K562 cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-09042 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: D7C2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

RHD Antibody (D7C2) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit RHD Antibody (D7C2) - Chimeric - Azide and BSA Free (NBP3-09042) is a recombinant monoclonal antibody validated for use in Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
• Isotype: IgG Lambda
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RHD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
  
  FC confirmed the binding of D7C2 to Rh D+ red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2 showed a percentage of 93% lysis (Edelman et al, 1997). D7C2 has been expressed recombinantly using a baculovirus-insect cell expression system (Edelman et al, 1997).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RHD Antibody (D7C2) - Chimeric - Azide and BSA Free

• blood group Rh(D) polypeptide
• CD240D
• DIIIc
• Rh blood group, D antigen
• RH
• RH30
• Rh4
• RHCED
• RhDCw
• RHDel
• RHDVA(TT)
• RhII
• RhK562-II
• RhPI
• RHPII
• RHXIII

Background

The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The classification of Rh positive and Rh negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Alternative splicing of the gene results in four transcript variants encoding four different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHD