PGM3 Antibody (OTI1A11)

Western Blot: PGM3 Antibody (1A11) [NBP2-03734] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PGM3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human prostate tissue using anti-PGM3 mouse monoclonal antibody.

Flow Cytometry: PGM3 Antibody (1A11) [NBP2-03734] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGM3 antibody, and then analyzed by flow cytometry.

Western Blot: PGM3 Antibody (1A11) [NBP2-03734] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGM3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGM3.

Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGM3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PGM3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human Kidney tissue using anti-PGM3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human liver tissue using anti-PGM3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PGM3 Antibody (1A11) [NBP2-03734] - Staining of paraffin-embedded Human pancreas tissue using anti-PGM3 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, Flow, IHC
• Clone: OTI1A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PGM3 Antibody (OTI1A11) Summary

• Immunogen: Full length human recombinant protein of human PGM3(NP_056414) produced in HKE293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGM3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 59.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGM3 Antibody (OTI1A11)

• AGM1PAGMAcetylglucosamine phosphomutase
• DKFZP434B187
• EC 5.4.2
• EC 5.4.2.3
• FLJ11614
• FLJ13623
• N-acetylglucosamine-phosphate mutase 1
• N-acetylglucosamine-phosphate mutase
• PGM 3
• phosphoacetylglucosamine mutase
• phosphoglucomutase 3
• Phosphoglucomutase-3

Background

This gene encodes a member of the phosphohexose mutase family. The encoded protein mediates both glycogen formation and utilization by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. A non-synonymous single nucleotide polymorphism in this gene may play a role in resistance to diabetic nephropathy and neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGM3
• Entrez:
  • 5238(Human)