PGM1 Antibody (3B8-H4)

Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - PGM1 monoclonal antibody (M01), clone 3B8-H4 Analysis of PGM1 expression in Jurkat.

Immunocytochemistry/Immunofluorescence: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of monoclonal antibody to PGM1 on HeLa cell. Antibody concentration 20 ug/ml.

Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi ( Cat # H00005236-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody (M01), clone 3B8-H4 (Cat # H00005236-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody (M01), clone 3B8-H4.Lane 1: PGM1 transfected lysate(61.4 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 transfected lysate using anti-PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: PGM1 Antibody (3B8-H4) [H00005236-M01] - Detection limit for recombinant GST tagged PGM1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu, Bv
• Applications: WB, ELISA, ICC/IF, IP, RNAi, S-ELISA
• Clone: 3B8-H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PGM1 Antibody (3B8-H4) Summary

• Immunogen: PGM1 (AAH19920, 1 a.a. ~ 562 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVKIVTVKTQAYQDQKPGTSGLRKRVKVFQSSANYAENFIQSIISTVEPAQRQEATLVVGGDGRFYMKEAIQLIARIAAANGIGRLVIGQNGILSTPAVSCIIRKIKAIGGIILTASHNPGGPNGDFGIKFNISNGGPAPEAITDKIFQISKTIEEYAVCPDLKVDLGVLGKQQFDLENKFKPFTVEIVDSVEAYATMLRSIFDFSALKELLSGPNRLKIRIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKYGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT
• Specificity: PGM1 - phosphoglucomutase 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • RNA Inhibition
  • Sandwich ELISA
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.
• Control:

  PGM1 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PGM1 Antibody (3B8-H4)

• EC 5.4.2
• EC 5.4.2.2
• Glucose phosphomutase 1
• GSD14
• PGM 1
• phosphoglucomutase 1
• phosphoglucomutase-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGM1 Antibody (H00005236-M01)(2)

Publications using H00005236-M01

• Yu Q, Wu W, Tian X et al. Unraveling proteome changes of Holstein beef M. semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry. J Proteomics 2016 Dec 28 [PMID: 28039026]
• Guillemin N, Bonnet M, Jurie C, Picard B. Functional analysis of beef tenderness. J Proteomics. 2011 Aug 09 [PMID: 21855665]

Bioinformatics

• Gene Symbol: PGM1
• Entrez:
  • 5236(Human)
• OMIM:
  • 171900(Human)
• Uniprot:
  • AAH19920(Human)