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PGM1 Antibody (3B8-H4)

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Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - PGM1 monoclonal antibody (M01), clone 3B8-H4 Analysis of PGM1 expression in Jurkat.
Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - PGM1 monoclonal antibody (M01), clone 3B8-H4 Analysis of PGM1 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of monoclonal antibody to PGM1 on HeLa cell. Antibody concentration 20 ug/ml.
Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi ( Cat # H00005236-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody (M01), clone 3B8-H4.Lane 1: PGM1 transfected lysate(61.4 KDa).Lane 2: ...read more
Immunoprecipitation: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 transfected lysate using anti-PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 MaxPab rabbit polyclonal ...read more
Sandwich ELISA: PGM1 Antibody (3B8-H4) [H00005236-M01] - Detection limit for recombinant GST tagged PGM1 is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP, RNAi
Clone
3B8-H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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PGM1 Antibody (3B8-H4) Summary

Immunogen
PGM1 (AAH19920, 1 a.a. - 562 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVKIVTVKTQAYQDQKPGTSGLRKRVKVFQSSANYAENFIQSIISTVEPAQRQEATLVVGGDGRFYMKEAIQLIARIAAANGIGRLVIGQNGILSTPAVSCIIRKIKAIGGIILTASHNPGGPNGDFGIKFNISNGGPAPEAITDKIFQISKTIEEYAVCPDLKVDLGVLGKQQFDLENKFKPFTVEIVDSVEAYATMLRSIFDFSALKELLSGPNRLKIRIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKYGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT
Specificity
PGM1 - phosphoglucomutase 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PGM1
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • RNA Inhibition
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.
Control
PGM1 Lysate (NBP2-66021)
Publications
Read Publications using H00005236-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PGM1 Antibody (3B8-H4)

  • EC 5.4.2
  • EC 5.4.2.2
  • Glucose phosphomutase 1
  • GSD14
  • PGM 1
  • phosphoglucomutase 1
  • phosphoglucomutase-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: ESD Antibody (J0-8) [NBP2-66825] - Analysis on different cell lysates using anti-ESD Mouse mAb. Positive control: Lane 1: K562 Lane 2: Jurkat
Immunocytochemistry/Immunofluorescence: ESD Antibody (J0-8) [NBP2-66825] - Staining of SW480 cells using anti-ESD Mouse mAb.
Immunohistochemistry-Paraffin: ESD Antibody (J0-8) [NBP2-66825] - Analysis of paraffin-embedded mouse embryo tissue using anti-ESD Mouse mAb.
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Western Blot: PGD Antibody [NBP1-31589] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: PGD Antibody [NBP1-31589] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PGD protein stained by PGD antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PGD Antibody [NBP1-31589] - protein at cytoplasm on A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft.
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Western Blot: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Analysis of Haptoglobin on different cells lysates using anti-Haptoglobin antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: HepG2
Immunocytochemistry/Immunofluorescence: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Staining Haptoglobin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Analysis of paraffin-embedded mouse lung tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.
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Western Blot: DLC1 Antibody [NBP1-88824] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: DLC1 Antibody [NBP1-88824] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: DLC1 Antibody [NBP1-88824] - Staining of human duodenum shows distinct positivity of microvilli in glandular cells.
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Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma cell line resistant to trabectedine. This image was submitted via customer Review.
Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. Figure is a representative of 3 independent experiments. Photo courtesy of Dr. Nagaraj, Case Western Reserve University.
Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - HeLa whole cell extracts.
(1 Review)
(2 Publications)
Species: Hu, Mu
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Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human colon, duodenum, lymph node and testis using Anti-ADA antibody NBP1-87404 (A) shows similar protein distribution across tissues to independent antibody NBP1-90361 (B).
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Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, human placenta tissue, TF‑1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat LMW‑PTP/ACP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5075) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMW‑PTP/ACP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Immunohistochemistry-Paraffin: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - Staining of colorectal adenocarcinoma with anti-FUT3/Blood Group Lewis A (7LE).
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Western Blot: GPT Antibody [NBP1-89111] - Analysis in human liver tissue.
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(1 Review)
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Publications for PGM1 Antibody (H00005236-M01)(2)


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Control Lysate(s)

PGM1 Knockout 293T Cell Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PGM1
Entrez
OMIM
Uniprot