PGD Antibody - BSA Free

Western Blot: PGD Antibody [NBP1-31589] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PGD Antibody [NBP1-31589] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PGD protein stained by PGD antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PGD Antibody [NBP1-31589] - protein at cytoplasm on A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft.

Western Blot: PGD Antibody [NBP1-31589] - Sample(30 ug whole cell lysate)A:293T B:MOLT4 C:Raji 10% SDS PAGE, antibody diluted at 1:1000.

Western Blot: PGD Antibody [NBP1-31589] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] diluted at 1:500.

Immunocytochemistry/Immunofluorescence: PGD Antibody [NBP1-31589] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGD protein stained by PGD antibody [N1N3] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Pm, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PGD Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PGD. The exact sequence is proprietary.
• Predicted Species: Rat (95%), Chicken (90%), Sheep (94%), Bovine (94%), Chimpanzee (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PGD
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (85%) and Zebrafish (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PGD Antibody - BSA Free

• 6PGD
• EC 1.1.1.44,6-phosphogluconate dehydrogenase, decarboxylating
• PGD
• PGDH
• phosphogluconate dehydrogenase

Background

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PGD Antibody (NBP1-31589)(4)

Publications using NBP1-31589

• Pushpak Bhattacharjee, Die Wang, Dovile Anderson, Joshua N Buckler, Eveline de Geus, Feng Yan, Galina Polekhina, Ralf Schittenhelm, Darren J Creek, Lawrence D Harris, Anthony J Sadler The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate The EMBO Journal 2024-05-22 [PMID: 38778156]
• Emmanuel Benichou, Bolaji Seffou, Selin Topçu, Ophélie Renoult, Véronique Lenoir, Julien Planchais, Caroline Bonner, Catherine Postic, Carina Prip-Buus, Claire Pecqueur, Sandra Guilmeau, Marie-Clotilde Alves-Guerra, Renaud Dentin The transcription factor ChREBP Orchestrates liver carcinogenesis by coordinating the PI3K/AKT signaling and cancer metabolism Nature Communications 2024-02-29 [PMID: 38424041]
• Elf S, Lin R, Xia S et al. Targeting 6-phosphogluconate dehydrogenase in the oxidative PPP sensitizes leukemia cells to antimalarial agent dihydroartemisinin. Oncogene 2016-06-06 [PMID: 27270429] (Human)
• Lemons JM, Feng XJ, Bennett BD et al. Quiescent fibroblasts exhibit high metabolic activity. PLoS Biol 2010-10-01 [PMID: 21049082] (WB)

Bioinformatics

• Gene Symbol: PGD