SOD2/Mn-SOD Antibody


Biological Strategies: Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, more
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. more
Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Western blot analysis of Rat Tissue lysates showing detection of SOD2/Mn-SOD protein using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Load: 15 ugprotein. more
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. more
Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - Immunohistochemistry analysis using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Gp, Gt, Ha, Pm, Rb, Sh, Sq, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

SOD2/Mn-SOD Antibody Summary

Recombinant Rat Mn SOD Protein
Mitochondrial matrix
Detects 25 kDa protein, corresponding to the molecular mass of Mn superoxide dismutase (SOD) on SDS-PAGE immunoblots.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:120
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:5000
Read Publications using
NB100-1992 in the following applications:

Reactivity Notes

Goat reactivity reported in scientific literature (PMID: 28797922). Reacts with Ciona intestinalis (Sea squirt).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS (pH 7.4), 50% Glycerol
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for SOD2/Mn-SOD Antibody

  • EC
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial


Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
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Applications: ICC, KO, Simple Western, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Hu, Rb, Rt
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Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: ICC, Simple Western, WB
Species: Hu
Applications: ELISA
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Am, Bv, Ca, Dr, Hu, Mu, Po, Rt, Sh
Applications: IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Gp, Gt, Ha, Pm, Rb, Sh, Sq, Xp
Applications: WB, ELISA, ICC/IF, IHC, IP

Publications for SOD2/Mn-SOD Antibody (NB100-1992)(38)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Chicken, Drosophila, Goat, Hamster, Rabbit, Sheep.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using NB100-1992 Applications Species
Chen H, Zhou J, Chen H et al. Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4 Clinical and translational medicine 2022-04-01 [PMID: 35390228] (WB, Mouse) WB Mouse
Peng L, Jiang J, Chen HN Et al. Redox-sensitive cyclophilin A elicits chemoresistance through realigning cellular oxidative status in colorectal cancer Cell reports 2021-11-30 [PMID: 34852234] (WB, Human) WB Human
Suresh V, Mohanty V, Avula K et al. Quantitative proteomics of hamster lung tissues infected with SARS-CoV-2 reveal host factors having implication in the disease pathogenesis and severity FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-07-01 [PMID: 34105201] (IF/IHC, Hamster) IF/IHC Hamster
Yu C, Chen S, Wang X et al. Exposure to maternal diabetes induces endothelial dysfunction and hypertension in adult male rat offspring Microvasc. Res. 2020-09-18 [PMID: 32956647] (WB, Rat) WB Rat
Zhang Q, Li J, Li Y et al. Bmi deficiency causes oxidative stress and intervertebral disc degeneration which can be alleviated by antioxidant treatment J. Cell. Mol. Med. 2020-06-24 [PMID: 32583517] (WB, Mouse) WB Mouse
Zhang H, Ma Y, Wang M et al. Dietary supplementation of l-arginine and N-carbamylglutamate enhances duodenal barrier and mitochondrial functions and suppresses duodenal inflammation and mitophagy in suckling lambs suffering from intrauterine-growth-restriction Food Funct. 2020-04-27 [PMID: 32374309]
Chen L, Yang R et al. 1,25-Dihydroxyvitamin D exerts an antiaging role by activation of Nrf2-antioxidant signaling and inactivation of p16/p53-senescence signaling. Aging Cell 2019-01-06 [PMID: 30907059] (IF/IHC, Mouse) IF/IHC Mouse
Zhang H, Jin Y, Wang M et Al. N-Carbamylglutamate and l-arginine supplementation improve hepatic antioxidant status in intrauterine growth-retarded suckling lambs RSC Adv. 2020-03-18 [PMID: 30869672]
Hagopian K, Tomilov AA, Tomilova N et al. Shc proteins influence the activities of enzymes involved in fatty acid oxidation and ketogenesis Metabolism 2012-06-07 [PMID: 22683097] (WB, Mouse) WB Mouse
Dumont M, Wille E, Stack C et al. Reduction of oxidative stress, amyloid deposition, memory deficit by manganese superoxide dismutase overexpression in a transgenic mouse model of Alzheimer's disease. FASEB J :fj.09-132928. 2009-01-01 [PMID: 19346295]
Show All 38 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOD2/Mn-SOD Antibody (NB100-1992). (Showing 1 - 2 of 2 FAQs).

  1. I want to measure SOD and Catalase on different erythroid subpopulation by using flow cytometry.I want to know whether these SOD and Catalase molecules are directly conjugated with fluorescence molecules or I have to do indirect staining.I have to use Ter 119 and CD71 molecule along with catalase or SO antibody.Can you please provide me detail protocol for using these antibodis or send me some references, if anyone used these antibodies by utilizing multicolour flow cytometry.
    • For our testing of NB100-1992, we used conjugated secondary antibodies. We have not yet tried to directly conjugate this primary, although this is certainly possible and would be expected to give the same results. We do not routinely test our antibodies in FACS, and in most cases, this application is validated by a trusted collaborator. For NB100-1992, viability for FACS was reported by a customer, and we do not have a detailed protocol for this application and product.
  2. What is your recommended working dilution for IHC?
    • In regards to the IHC dilution, we would recommend starting at about 1:10-1:2000. Obviously this will depend on your samples and how highly expressed the protein is in them. You may need to optimize, but this is a good starting point.

Secondary Antibodies


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Gene Symbol SOD2