SOD2/Mn-SOD Antibody

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Biological Strategies: Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, ...read more
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. ...read more
Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis of Rat Tissue lysates showing detection of SOD2 protein using Rabbit Anti-SOD2 Polyclonal Antibody. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. ...read more
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. ...read more
Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NB100-1992] - Superoxide Dismutase 2 Antibody [NB100-1992] - Analysis of mouse back skin.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Gp, Gt, Ha, Pm, Rb, Sh, Sq, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

SOD2/Mn-SOD Antibody Summary

Immunogen
Rat Mn SOD (NP_058747.1).
Localization
Mitochondrial matrix
Specificity
Detects 25kDa protein, corresponding to the molecular mass of Mn superoxide dismutase (SOD) on SDS-PAGE immunoblots.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Control
Rat Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB100-1992 in the following applications:

  • 4 publications
  • WB
    17 publications

Reactivity Notes

Goat reactivity reported in scientific literature (PMID: 28797922). Reacts with Ciona intestinalis (Sea squirt).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOD2/Mn-SOD Antibody

  • EC 1.15.1.1
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial

Background

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Rt, Rb
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Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, KO
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Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
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Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
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Species: Hu
NB100-56098
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP
NB100-56503
Species: Hu, Mu, Rt, Dr, Rb
Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, Flow-CS, Flow-IC
NBP2-45626
Species: Hu
Applications: WB, IHC, IHC-P
NB100-56708
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KO
NB300-500
Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr, Sh
Applications: WB, IHC, IHC-Fr, IHC-P
NB100-1992
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Gp, Gt, Ha, Pm, Rb, Sh, Sq, Xp
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for SOD2/Mn-SOD Antibody (NB100-1992)(32)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chicken, Drosophila, Goat, Rabbit, Sheep.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(4)
WB
(17)
All Applications
Filter By Species
Human
(2)
Mouse
(8)
Rat
(2)
Chicken
(1)
Drosophila
(1)
Goat
(2)
Rabbit
(2)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB100-1992 Applications Species
Zhang Q, Li J, Li Y et al. Bmi deficiency causes oxidative stress and intervertebral disc degeneration which can be alleviated by antioxidant treatment J. Cell. Mol. Med. Jun 24 2020 [PMID: 32583517] (WB, Mouse) WB Mouse
Zhang H, Ma Y, Wang M et al. Dietary supplementation of l-arginine and N-carbamylglutamate enhances duodenal barrier and mitochondrial functions and suppresses duodenal inflammation and mitophagy in suckling lambs suffering from intrauterine-growth-restriction Food Funct. Apr 27 2020 [PMID: 32374309]
Aral C, Demirkesen S, Bircan R, Yasar Sirin D Melatonin reverses the oxidative stress and mitochondrial dysfunction caused by LETM1 silencing Cell Biol. Int. Nov 27 2019 [PMID: 31777134] (WB, Mouse) WB Mouse
Sun H, Qiao W, Cui M et al. The Polycomb Protein Bmi1 Plays a Crucial Role in the Prevention of 1,25(OH)2 D Deficiency-induced Bone Loss J Bone Miner Res. Nov 14 2019 [PMID: 31725940] (IHC-P, WB, Mouse) IHC-P, WB Mouse
Chen YH, Lin WW, Liu CS, Su SL. H2O2 induces caveolin-1 degradation and impaired mitochondrial function in E11 podocytes Mol Med Rep 2017 Sep 18 [PMID: 28944844] (WB, Human) WB Human
Messner H An in vivo, ex vivo, and in vitro exploration of the use of chronic hypoxia/physioxia and ROS/RNS-mediated alteration of physiological function in mitochondrial disease Thesis Jan 1 2020 (WB, Human) WB Human
Zhang H, Jin Y, Wang M et Al. N-Carbamylglutamate and l-arginine supplementation improve hepatic antioxidant status in intrauterine growth-retarded suckling lambs RSC Adv. Mar 18 2020 [PMID: 30869672]
Xie MY, Yang Y, Liu P et al. 5-aza-2'-deoxycytidine in the regulation of antioxidant enzymes in retinal endothelial cells and rat diabetic retina. Int J Ophthalmol Jan 18 2019 [PMID: 30662833] (WB, Rat) WB Rat
Du Y, Gu X, Meng H et al. Muscone improves cardiac function in mice after myocardial infarction by alleviating cardiac macrophage-mediated chronic inflammation through inhibition of NF-kB and NLRP3 inflammasome. Am J Transl Res Dec 15 2018 [PMID: 30662666] (WB, Mouse) WB Mouse
Geng Q, Gao H, Yang R et al. Pyrroloquinoline Quinone Prevents Estrogen Deficiency-Induced Osteoporosis by Inhibiting Oxidative Stress and Osteocyte Senescence Int. J. Biol. Sci. Jan 1 2019 [PMID: 30662347] (WB, Mouse) WB Mouse
Show All 32 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOD2/Mn-SOD Antibody (NB100-1992). (Showing 1 - 2 of 2 FAQs).

  1. I want to measure SOD and Catalase on different erythroid subpopulation by using flow cytometry.I want to know whether these SOD and Catalase molecules are directly conjugated with fluorescence molecules or I have to do indirect staining.I have to use Ter 119 and CD71 molecule along with catalase or SO antibody.Can you please provide me detail protocol for using these antibodis or send me some references, if anyone used these antibodies by utilizing multicolour flow cytometry.
    • For our testing of NB100-1992, we used conjugated secondary antibodies. We have not yet tried to directly conjugate this primary, although this is certainly possible and would be expected to give the same results. We do not routinely test our antibodies in FACS, and in most cases, this application is validated by a trusted collaborator. For NB100-1992, viability for FACS was reported by a customer, and we do not have a detailed protocol for this application and product.
  2. What is your recommended working dilution for IHC?
    • In regards to the IHC dilution, we would recommend starting at about 1:10-1:2000. Obviously this will depend on your samples and how highly expressed the protein is in them. You may need to optimize, but this is a good starting point.

Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SOD2
Entrez
Uniprot