Alkaline Phosphatase/ALPL Antibody [Unconjugated]


Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen more
Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # more
Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 more
Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly­clonal Antibody more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, ICC/IF

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Catalog# & Formulation Size Price

Alkaline Phosphatase/ALPL Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse Alkaline Phosphatase/ALPL
Accession # P09242
Detects mouse Alkaline Phosphatase/ALPL in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF2910 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody [Unconjugated]

  • Akp2
  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • ALPL
  • EC
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALPMGC167935


Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP) (1). The Alpl gene is a key regulator of bone mineralization in mice (2). A variety of mutations in the human ALPL gene leads to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 504 to 524) is not present in the mature form.

  1. Terao, M. and B. Mintz (1987) Proc. Natl. Acad. Sci. USA 84:7051.
  2. Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9445.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase/ALPL Antibody (AF2910)(27)

We have publications tested in 4 confirmed species: Human, Mouse, Rat,  Human.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.

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Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using AF2910 Applications Species
Yu, Y;Rong, K;Yao, D;Zhang, Q;Cao, X;Rao, B;Xia, Y;Lu, Y;Shen, Y;Yao, Y;Xu, H;Ma, P;Cao, Y;Qin, A; The structural pathology for hypophosphatasia caused by malfunctional tissue non-specific alkaline phosphatase Nature communications 2023-07-08 [PMID: 37422472] (Western Blot, Human) Western Blot  Human
Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration Cell reports 2023-06-16 [PMID: 37330908]
Z Awida, S Hiram-Bab, A Bachar, H Saed, D Zyc, A Gorodov, N Ben-Califa, S Omari, J Omar, L Younis, JA Iden, L Graniewitz, I Gluzman, T Liron, B Raphael-Mi, A Kolomansky, M Rauner, B Wielockx, Y Gabet, D Neumann Erythropoietin Receptor (EPOR) Signaling in the Osteoclast Lineage Contributes to EPO-Induced Bone Loss in Mice International Journal of Molecular Sciences, 2022-10-10;23(19):. 2022-10-10 [PMID: 36233351] (Flow Cytometry, Mouse) Flow Cytometry Mouse
E Puissant, F Gilis, V Tevel, JM Vandeweerd, B Flamion, M Jadot, M Boonen Hyaluronidase 1 deficiency decreases bone mineral density in mice Oncogene, 2022-06-16;12(1):10142. 2022-06-16 [PMID: 35710820] (IHC, Mouse) IHC Mouse
S Lin, S Yin, J Shi, G Yang, X Wen, W Zhang, M Zhou, X Jiang Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration Bioactive materials, 2022-03-24;18(0):116-127. 2022-03-24 [PMID: 35387176] (IHC, Mouse) IHC Mouse
Y Chen, H Wang, Q Yang, W Zhao, Y Chen, Q Ni, W Li, J Shi, W Zhang, L Li, Y Xu, H Zhang, D Miao, L Xing, W Sun Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis Theranostics, 2022-01-01;12(3):1074-1096. 2022-01-01 [PMID: 35154475] (IHC, Human) IHC Human
Z Awida, A Bachar, H Saed, A Gorodov, N Ben-Califa, M Ibrahim, A Kolomansky, JA Iden, L Graniewitz, T Liron, S Hiram-Bab, M Brines, Y Gabet, D Neumann The Non-Erythropoietic EPO Analogue Cibinetide Inhibits Osteoclastogenesis In Vitro and Increases Bone Mineral Density in Mice International Journal of Molecular Sciences, 2021-12-21;23(1):. 2021-12-21 [PMID: 35008482] (Flow Cytometry, Mouse) Flow Cytometry Mouse
LS Chen, M Zhang, P Chen, XF Xiong, PQ Liu, HB Wang, JJ Wang, J Shen The m6A demethylase FTO promotes the osteogenesis of mesenchymal stem cells by downregulating PPARG Acta pharmacologica Sinica, 2021-08-30;0(0):. 2021-08-30 [PMID: 34462564] (IHC, Mouse) IHC Mouse
Y Sun, JF Rahbani, MP Jedrychows, CL Riley, S Vidoni, D Bogoslavsk, B Hu, PA Dumesic, X Zeng, AB Wang, NH Knudsen, CR Kim, A Marasciull, JL Millán, ET Chouchani, L Kazak, BM Spiegelman Mitochondrial TNAP controls thermogenesis by hydrolysis of phosphocreatine Nature, 2021-05-12;0(0):. 2021-05-12 [PMID: 33981039] (Western Blot, Mouse) Western Blot Mouse
X Yang, F Zhou, P Yuan, G Dou, X Liu, S Liu, X Wang, R Jin, Y Dong, J Zhou, Y Lv, Z Deng, S Liu, X Chen, Y Han, Y Jin T cell-depleting nanoparticles ameliorate bone loss by reducing activated T cells and regulating the Treg/Th17 balance Bioactive materials, 2021-03-10;6(10):3150-3163. 2021-03-10 [PMID: 33778195] (Western Blot, Mouse) Western Blot Mouse
Show All 27 Publications.

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Gene Symbol ALPL