Alkaline Phosphatase/ALPL Antibody [Unconjugated]

Images

 
Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen ...read more
Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly­clonal Antibody ...read more
Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # ...read more
Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Alkaline Phosphatase/ALPL Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Alkaline Phosphatase/ALPL
Phe18-Gly503
Accession # P09242
Specificity
Detects mouse Alkaline Phosphatase/ALPL in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross‑reactivity with recombinant human ALPL is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALPL
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF2910 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody [Unconjugated]

  • Akp2
  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • ALPL
  • APTNAP
  • AP-TNAP
  • EC 3.1.3.1
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALP
  • TNSALPMGC167935

Background

Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP) (1). The Alpl gene is a key regulator of bone mineralization in mice (2). A variety of mutations in the human ALPL gene leads to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 504 to 524) is not present in the mature form.

  1. Terao, M. and B. Mintz (1987) Proc. Natl. Acad. Sci. USA 84:7051.
  2. Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9445.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase/ALPL Antibody (AF2910)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: IHC, IHC - Paraffin embedded, IHC-F, WB.


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IHC
(2)
IHC - Paraffin embedded
(1)
IHC-F
(1)
WB
(2)
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Mouse
(6)
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Showing Publications 1 - 6 of 6.
Publications using AF2910 Applications Species
RM Sterner, KN Kremer, A Dudakovic, JJ Westendorf, AJ van Wijnen, KE Hedin Tissue-Nonspecific Alkaline Phosphatase Is Required for MC3T3 Osteoblast-Mediated Protection of Acute Myeloid Leukemia Cells from Apoptosis J. Immunol., 2018;0(0):. 2018 [PMID: 29914885] (WB,, Mouse) WB Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC,, Mouse) IHC Mouse
Arjun Deb Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification Cell Stem Cell, 2016;0(0):. 2016 [PMID: 27867037] (IHC-F,, Mouse) IHC-F Mouse
Yoshizaki, Keigo, Hu, Lizhi, Nguyen, Thai, Sakai, Kiyoshi, He, Bing, Fong, Chak, Yamada, Yoshihik, Bikle, Daniel D, Oda, Yuko Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair. PLoS ONE, 2014;9(6):e99991. 2014 [PMID: 24949995] (IHC - Paraffin embedded,, Mouse) IHC - Paraffin embedded Mouse
Isaac, J, Erthal, J, Gordon, J, Duverger, O, Sun, H-W, Lichtler, A C, Stein, G S, Lian, J B, Morasso, M I DLX3 regulates bone mass by targeting genes supporting osteoblast differentiation and mineral homeostasis in vivo. Cell Death Differ, 2014;21(9):1365-76. 2014 [PMID: 24948010] (IHC,, Mouse) IHC Mouse
Liedert A, Mattausch L, Rontgen V, Blakytny R, Vogele D, Pahl M, Bindl R, Neunaber C, Schinke T, Harroch S, Amling M, Ignatius A Midkine-deficiency increases the anabolic response of cortical bone to mechanical loading. Bone, 2011;48(4):945-51. 2011 [PMID: 21185956] (WB,, Mouse) WB Mouse

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Bioinformatics

Gene Symbol ALPL
Uniprot