Alkaline Phosphatase/ALPL Antibody [Unconjugated]

Images

 
Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen ...read more
Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly­clonal Antibody ...read more
Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # ...read more
Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Alkaline Phosphatase/ALPL Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Alkaline Phosphatase/ALPL
Phe18-Gly503
Accession # P09242
Specificity
Detects mouse Alkaline Phosphatase/ALPL in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALPL
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2910 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody [Unconjugated]

  • Akp2
  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • ALPL
  • APTNAP
  • AP-TNAP
  • EC 3.1.3.1
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALP
  • TNSALPMGC167935

Background

Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP) (1). The Alpl gene is a key regulator of bone mineralization in mice (2). A variety of mutations in the human ALPL gene leads to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 504 to 524) is not present in the mature form.

  1. Terao, M. and B. Mintz (1987) Proc. Natl. Acad. Sci. USA 84:7051.
  2. Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9445.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-3638
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
DPI00
Species: Hu
Applications: ELISA
DCC270
Species: Hu
Applications: ELISA
NBP1-89133
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89131
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-32263
Species: Hu, Mu
Applications: IHC, IP, WB
NBP1-89134
Species: Hu
Applications: IHC, IHC-P
NBP2-27561
Species: Ch, Hu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
AF808
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, IHC, Neut, WB
NBP2-48480
Species: Hu
Applications: Flow-CS, Flow, IHC, IHC-Fr, IHC-P
MAB1419
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
AF6240
Species: Hu
Applications: ELISA, IHC, WB
NBP1-31348
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-06185
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00000248-M03
Species: Hu
Applications: ELISA, WB

Publications for Alkaline Phosphatase/ALPL Antibody (AF2910)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(4)
IHC
(6)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(15)
Rat
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using AF2910 Applications Species
Z Awida, A Bachar, H Saed, A Gorodov, N Ben-Califa, M Ibrahim, A Kolomansky, JA Iden, L Graniewitz, T Liron, S Hiram-Bab, M Brines, Y Gabet, D Neumann The Non-Erythropoietic EPO Analogue Cibinetide Inhibits Osteoclastogenesis In Vitro and Increases Bone Mineral Density in Mice International Journal of Molecular Sciences, 2021;23(1):. 2021 [PMID: 35008482] (Flow Cytometry, Mouse) Flow Cytometry Mouse
LS Chen, M Zhang, P Chen, XF Xiong, PQ Liu, HB Wang, JJ Wang, J Shen The m6A demethylase FTO promotes the osteogenesis of mesenchymal stem cells by downregulating PPARG Acta pharmacologica Sinica, 2021;0(0):. 2021 [PMID: 34462564] (IHC, Mouse) IHC Mouse
Y Sun, JF Rahbani, MP Jedrychows, CL Riley, S Vidoni, D Bogoslavsk, B Hu, PA Dumesic, X Zeng, AB Wang, NH Knudsen, CR Kim, A Marasciull, JL Millán, ET Chouchani, L Kazak, BM Spiegelman Mitochondrial TNAP controls thermogenesis by hydrolysis of phosphocreatine Nature, 2021;0(0):. 2021 [PMID: 33981039] (Western Blot, Mouse) Western Blot Mouse
X Yang, F Zhou, P Yuan, G Dou, X Liu, S Liu, X Wang, R Jin, Y Dong, J Zhou, Y Lv, Z Deng, S Liu, X Chen, Y Han, Y Jin T cell-depleting nanoparticles ameliorate bone loss by reducing activated T cells and regulating the Treg/Th17 balance Bioactive materials, 2021;6(10):3150-3163. 2021 [PMID: 33778195] (Western Blot, Mouse) Western Blot Mouse
S Yu, J Guo, Z Sun, C Lin, H Tao, Q Zhang, Y Cui, H Zuo, Y Lin, S Chen, H Liu, Z Chen BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation Cell Death & Disease, 2021;12(2):197. 2021 [PMID: 33608506] (IHC, Mouse) IHC Mouse
S Takeyari, T Kubota, Y Ohata, M Fujiwara, T Kitaoka, Y Taga, K Mizuno, K Ozono 4-phenylbutyric acid enhances the mineralization of osteogenesis imperfecta iPSC-derived osteoblasts J Biol Chem, 2020;0(0):. 2020 [PMID: 33154166] (Western Blot, Human) Western Blot Human
L Zhang, M Qi, J Chen, J Zhao, L Li, J Hu, Y Jin, W Liu Impaired autophagy triggered by HDAC9 in mesenchymal stem cells accelerates bone mass loss Stem Cell Res Ther, 2020;11(1):269. 2020 [PMID: 32620134] (Western Blot, Human) Western Blot Human
X Sun, B Wei, Z Peng, X Chen, Q Fu, C Wang, J Zhen, J Sun A polysaccharide from the dried rhizome of Drynaria fortunei (Kunze) J. Sm. prevents ovariectomized (OVX)-induced osteoporosis in rats J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32065504] (Rat) Rat
L Yin, ZX Zhou, M Shen, N Chen, F Jiang, SL Wang The Human Amniotic Mesenchymal Stem Cells (hAMSCs) Improve the Implant Osseointegration and Bone Regeneration in Maxillary Sinus Floor Elevation in Rabbits Stem Cells Int, 2019;2019(0):9845497. 2019 [PMID: 31885631] (ICC, Human) ICC Human
T Kazi, I Niibe, A Nishikawa, T Matsuzaki Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers FEBS Open Bio, 2019;0(0):. 2019 [PMID: 31730301] (ICC, Mouse) ICC Mouse
Show All 20 Publications.

Reviews for Alkaline Phosphatase/ALPL Antibody (AF2910) (0)

There are no reviews for Alkaline Phosphatase/ALPL Antibody (AF2910). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Alkaline Phosphatase/ALPL Antibody (AF2910) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Alkaline Phosphatase/ALPL Products

Bioinformatics Tool for Alkaline Phosphatase/ALPL Antibody (AF2910)

Discover related pathways, diseases and genes to Alkaline Phosphatase/ALPL Antibody (AF2910). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Alkaline Phosphatase/ALPL Antibody (AF2910)

Discover more about diseases related to Alkaline Phosphatase/ALPL Antibody (AF2910).
 

Pathways for Alkaline Phosphatase/ALPL Antibody (AF2910)

View related products by pathway.

PTMs for Alkaline Phosphatase/ALPL Antibody (AF2910)

Learn more about PTMs related to Alkaline Phosphatase/ALPL Antibody (AF2910).

Blogs on Alkaline Phosphatase/ALPL

There are no specific blogs for Alkaline Phosphatase/ALPL, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Alkaline Phosphatase/ALPL Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol ALPL
Uniprot