Alkaline Phosphatase/ALPL Antibody [Unconjugated]


Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen more
Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly­clonal Antibody more
Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # more
Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready, ICC/IF

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Alkaline Phosphatase/ALPL Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse Alkaline Phosphatase/ALPL
Accession # P09242
Detects mouse Alkaline Phosphatase/ALPL in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross‑reactivity with recombinant human ALPL is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Read Publications using
AF2910 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody [Unconjugated]

  • Akp2
  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • ALPL
  • EC
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALPMGC167935


Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP) (1). The Alpl gene is a key regulator of bone mineralization in mice (2). A variety of mutations in the human ALPL gene leads to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 504 to 524) is not present in the mature form.

  1. Terao, M. and B. Mintz (1987) Proc. Natl. Acad. Sci. USA 84:7051.
  2. Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9445.
  3. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IP
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Rt, Ch
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
Species: Hu, Po
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, IHC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, Flow, CyTOF-ready

Publications for Alkaline Phosphatase/ALPL Antibody (AF2910)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.

Filter By Application
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF2910 Applications Species
L Zhang, M Qi, J Chen, J Zhao, L Li, J Hu, Y Jin, W Liu Impaired autophagy triggered by HDAC9 in mesenchymal stem cells accelerates bone mass loss Stem Cell Res Ther, 2020;11(1):269. 2020 [PMID: 32620134] (Western Blot, Human) Western Blot Human
L Yin, ZX Zhou, M Shen, N Chen, F Jiang, SL Wang The Human Amniotic Mesenchymal Stem Cells (hAMSCs) Improve the Implant Osseointegration and Bone Regeneration in Maxillary Sinus Floor Elevation in Rabbits Stem Cells Int, 2019;2019(0):9845497. 2019 [PMID: 31885631] (ICC, Human) ICC Human
T Kazi, I Niibe, A Nishikawa, T Matsuzaki Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers FEBS Open Bio, 2019;0(0):. 2019 [PMID: 31730301] (ICC, Mouse) ICC Mouse
N Charoenpha, R Aeimlapa, S Sooksawanw, J Thongbunch, J Teerapornp, S Svasti, K Wongdee Responses of primary osteoblasts and osteoclasts from hemizygous beta-globin knockout thalassemic mice with elevated plasma glucose to 1,25-dihydroxyvitamin D3 Sci Rep, 2019;9(1):13963. 2019 [PMID: 31562377] (ICC, Mouse) ICC Mouse
W Liu, L Zhang, K Xuan, C Hu, L Li, Y Zhang, F Jin, Y Jin Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3? Complex in Hypophosphatasia Theranostics, 2018;8(20):5575-5592. 2018 [PMID: 30555565] (ICC, Human) ICC Human
MJ Carr, JS Toma, APW Johnston, PE Steadman, SA Yuzwa, N Mahmud, PW Frankland, DR Kaplan, FD Miller Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30503141] (IHC, Mouse) IHC Mouse
RM Sterner, KN Kremer, A Dudakovic, JJ Westendorf, AJ van Wijnen, KE Hedin Tissue-Nonspecific Alkaline Phosphatase Is Required for MC3T3 Osteoblast-Mediated Protection of Acute Myeloid Leukemia Cells from Apoptosis J. Immunol., 2018;0(0):. 2018 [PMID: 29914885] (Western Blot, Mouse) Western Blot Mouse
G Giovannell, G Giacomazzi, H Grosemans, M Sampaolesi Morphological and functional analyses of skeletal muscles from an immunodeficient animal model of limb girdle muscular dystrophy type 2E Muscle Nerve, 2018;0(0):. 2018 [PMID: 29476695] (IHC-Fr, Mouse) IHC-Fr Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC, Mouse) IHC Mouse
Arjun Deb Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification Cell Stem Cell, 2016;0(0):. 2016 [PMID: 27867037] (IHC, Mouse) IHC Mouse
Show All 13 Publications.

Reviews for Alkaline Phosphatase/ALPL Antibody (AF2910) (0)

There are no reviews for Alkaline Phosphatase/ALPL Antibody (AF2910). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Alkaline Phosphatase/ALPL Antibody (AF2910) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional Alkaline Phosphatase/ALPL Products

Bioinformatics Tool for Alkaline Phosphatase/ALPL Antibody (AF2910)

Discover related pathways, diseases and genes to Alkaline Phosphatase/ALPL Antibody (AF2910). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Alkaline Phosphatase/ALPL Antibody (AF2910)

Discover more about diseases related to Alkaline Phosphatase/ALPL Antibody (AF2910).

Pathways for Alkaline Phosphatase/ALPL Antibody (AF2910)

View related products by pathway.

PTMs for Alkaline Phosphatase/ALPL Antibody (AF2910)

Learn more about PTMs related to Alkaline Phosphatase/ALPL Antibody (AF2910).

Blogs on Alkaline Phosphatase/ALPL

There are no specific blogs for Alkaline Phosphatase/ALPL, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Alkaline Phosphatase/ALPL Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol ALPL