Tissue alpha-L-Fucosidase/FUCA1 Antibody (810607)

Western blot shows lysates of human placenta tissue and human thyroid tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Tissue a-L-Fucosidase/FUCA1 Monoclonal Antibody (Catalog # MAB7039) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tissue a-L-Fucosidase/FUCA1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human thyroid tissue, loaded at 0.5 mg/mL. A specific band was detected for Tissue alpha ‑L‑Fucosidase/FUCA1 at approximately 56 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Tissue alpha ‑L‑Fucosidase/FUCA1 Monoclonal Antibody (Catalog # MAB7039). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 810607
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Tissue alpha-L-Fucosidase/FUCA1 Antibody (810607) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Tissue alpha ‑L‑Fucosidase/FUCA1
  Gln32-Lys466
  Accession # P04066
• Specificity: Detects human Tissue alpha ‑L‑Fucosidase/FUCA1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUCA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tissue alpha-L-Fucosidase/FUCA1 Antibody (810607)

• Alpha-L-fucosidase 1
• Alpha-L-fucosidase I
• Alpha-L-fucoside fucohydrolase 1
• EC 3.2.1
• EC 3.2.1.51
• FUCA
• FUCA1
• fucosidase, alpha-L- 1, tissue
• Tissue alphaLFucosidase
• Tissue alpha-L-Fucosidase

Background

Fucosylated glycoconjugates play numerous roles in biological processes, such as development, apoptosis and the immune responses, and are involved in the pathology of inflammation, cancer and cystic fibrosis (1, 2, 3, 4). Tissue alpha ‑L‑Fucosidase, also known as FUCA1, is a lysosomal enzyme responsible for hydrolyzing alpha ‑L‑fucose moieties from glycolipids and oligosaccharides (5). The mature enzyme in the liver has a molecular weight of 200 kDa and exists in the native state as a tetramer (6). Defects in FUCA1 are the cause of fucosidosis (7, 8, 9), an autosomal recessive lysosomal storage disease characterized by the accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Serum alpha ‑L‑fucosidase has been identified as a useful marker for oral cancer (10).

1. Hiraishi, K. et al. (1993) Glycobiology 3:381.
2. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
3. Becker, D.J. and Lowe, J.B. (2003) Glycobiology 13:41R.
4. Liu, T.W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:14581.
5. Fukushima, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:1262.
6. Alhadeff, J.A. et al. (1975) J. Biol. Chem. 250:7106.
7. O’Brien, J.S. et al. (1987) Enzyme 38:45.
8. Durand, P. et al. (1969) J. Pediatr. 75:665.
9. Johnson, K. and Dawson, G. (1985) Biochem. Biophys. Res. Commun. 133:90.
10. Shah, M. et al. (2008) Cancer 113:336.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUCA1
• Uniprot:
  • P04066()