TIM-1/KIM-1/HAVCR Antibody - BSA Free

Western Blot: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Analysis of TIM-1/KIM-1/HAVCR in human brain lysate in the A) absence and B) presence of immunizing peptide, and C) mouse brain lysate using TIM-1 antibody at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Caco-2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-TIM-1/KIM-1/HAVCR Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TIM-1/KIM-1/HAVCR Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TIM-1/KIM-1/HAVCR Antibody - BSA Free (NB100-56421) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-TIM-1/KIM-1/HAVCR Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide (SPLYSYTTDGNDTVT) of human TIM-1 protein was used as immunogen for this antibody. This sequence corresponds to amino acids (aa) 248-262 of NP_036338 and aa 84-98 of EAW61615.1. Isoforms of TIM-1 have been described. For example, NP_036338 is 364 aa and EAW61615.1 is 194 aa. EAW61615.1 is referred to as isoform CRA_a.
• Specificity: Isoforms of different molecular weights have been described for TIM-1. The sequence used for immunogen is conserved between TIM-1 (364aa, NP_036338) and TIM-1 isoform CRA_a (194aa, EAW61615.1). Therefore, the molecular weight observed in western blots may vary depending on the forms present.
• Predicted Species: Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HAVCR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1-3 ug/ml
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted to react with New World Monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TIM-1/KIM-1/HAVCR Antibody - BSA Free

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

Official Gene Symbol HAVCR1 Gene ID 26762(Human) Gene Map Locus 5q33.2(Human) HAVCR1, a 359 A.A Class 1 integral membrane glycoprotein, is used by Hepatitis A virus to infect humans. It consists of a conserved N-terminal Cys-rich region, IgV (Immunoglobulin Variable region like) domain and a TSP-rich mucin domain in the extracellular region followed by a highly conserved tyrosine kinase phosphorylation motif in the cytoplasmic region. HAVCR1 is expressed on terminally differentiated CD4+ Th2 cells and regulates cytokine production. It plays an important role in the regulation of immune responses in the development of asthma and allergic responses. Its expression is critically upregulated in dedifferentiated kidney cells, thus indicating a possible role in regeneration of proximal tubule epithelium in Kidneys. HAVCR1 has shown to be upregulated in renal carcinomas, suggesting its role in tumor progression and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-1/KIM-1/HAVCR Antibody (NB100-56421)(3)

Publications using NB100-56421

• Yang CC, Sung PH, Chen CH Et al. Additional benefit of induced pluripotent stem cell-derived mesenchymal stem cell therapy on sepsis syndrome-associated acute kidney injury in rat treated with antibiotic Stem cell research & therapy 2021-10-07 [PMID: 34620235] (IHC-P, Rat)
• Xu G, Cheng L, Lu L et al. Expression of T-cell immunoglobulin- and mucin-domain-containing molecule-1 (TIM-1) is increased in a mouse model of asthma and relationship to GATA-3. Life Sci. 2008-03-12 [PMID: 18234236] (WB, Mouse)
  
  Details:
  WB (mouse spleen tissue), Figs. 6, 7.
• Xu G, Cheng L, Wen W et al. Inverse association between T-cell immunoglobulin and mucin domain-1 and T-bet in a mouse model of allergic rhinitis. Laryngoscope. 2007-06-01 [PMID: 17460580] (WB, Mouse)
  
  Details:
  WB (mouse spleen tissue), Figs. 6, 7.

Bioinformatics

• Gene Symbol: HAVCR1
• Entrez:
  • 26762(Human)
  • 171283(Mouse)