Timeless Antibody

Images

 
Western Blot: Timeless Antibody [NB100-40853] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more
Immunohistochemistry: Timeless Antibody [NB100-40853] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless (Cat. No. NB100-40853) used at a dilution of 1:200 (1ug/ml). ...read more
Western Blot: Timeless Antibody [NB100-40853] - Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Timeless antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Timeless Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1170 and the C-terminus (residue 1208) of human Timeless using the numbering given in entry NP_003911.1 (GeneID 8914).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TIMELESS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:200- 1:1000
  • Immunohistochemistry-Paraffin 1:200- 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Immunohistochemistry 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Timeless Lysate (NBL1-16915)
Reviewed Applications
Read 1 Review rated 4
using
NB100-40853 in the following applications:

Publications
Read Publications using
NB100-40853 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Timeless Antibody

  • FLJ12640
  • hTIMTIMELESS1
  • TIM1protein timeless homolog
  • timeless (Drosophila) homolog
  • timeless homolog (Drosophila)
  • TIMFLJ20714

Background

Timeless plays a role in DNA damage checkpoint responses. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein (referenced from entrez gene).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Timeless Antibody (NB100-40853)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
(1)
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Review for Timeless Antibody (NB100-40853) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-40853:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23359676
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23359676

Details

Detection NotesSee PMID 23359676

Comments

CommentsPublished in PMID: 23359676

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for Timeless Antibody (NB100-40853)

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Recent Reviews

4
5
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4
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1
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Anonymous
12/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TIMELESS
Entrez
Uniprot