Timeless Antibody

Western Blot: Timeless Antibody [NB100-40853] - Detection of human and mouse Timeless by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Timeless antibody NB100-40853 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry: Timeless Antibody [NB100-40853] - Detection of human Timeless by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless (NB100-40853). Detection: DAB

Western Blot: Timeless Antibody [NB100-40853] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Timeless antibody NB100-40853 used for IP at 6 ug per reaction. Timeless was also immunoprecipitated by rabbit anti-Timeless antibody NB100-40852. For blotting immunoprecipitated Timeless, NB100-40853 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: Timeless Antibody [NB100-40853] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-Timeless antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescencewith an exposure time of 75 seconds.

Immunohistochemistry-Paraffin: Timeless Antibody [NB100-40853] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Timeless Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1170 and the C-terminus (residue 1208) of human Timeless using the numbering given in entry NP_003911.1 (GeneID 8914).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TIMELESS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 139 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Timeless Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Timeless Antibody

• FLJ12640
• hTIMTIMELESS1
• TIM1protein timeless homolog
• timeless (Drosophila) homolog
• timeless homolog (Drosophila)
• TIMFLJ20714

Background

Timeless plays a role in DNA damage checkpoint responses. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein (referenced from entrez gene).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Timeless Antibody (NB100-40853)(2)

Publications using NB100-40853

• Cho WH, Kang YH, An YY et al. Human Tim-Tipin complex affects the biochemical properties of the replicative DNA helicase and DNA polymerases Proc Natl Acad Sci U S A. 2013-02-12 [PMID: 23359676] (WB, Human)
• Dheekollu J, Lieberman PM. The replisome pausing factor Timeless is required for episomal maintenance of latent Epstein-Barr virus. J Virol 2011-06-01 [PMID: 21490103]

Review for Timeless Antibody (NB100-40853) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23359676
• Species: Human

Bioinformatics

• Gene Symbol: TIMELESS
• Entrez:
  • 8914(Human)
• Uniprot:
  • Q9UNS1(Human)