Mouse/Rat Cystatin C Quantikine ELISA Kit

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat Cystatin C Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse/Rat Cystatin C Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat Cystatin C in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Cystatin C and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obt...ained using natural mouse or rat Cystatin C showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse and rat Cystatin C.
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• Specificity: Natural and recombinant mouse and rat Cystatin C
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Cst3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat Cystatin C Quantikine ELISA Kit

• ARMD11
• bA218C14.4 (cystatin C)
• CST3
• cystatin 3
• cystatin C (amyloid angiopathy and cerebral hemorrhage)
• Cystatin C
• cystatin-3
• cystatin-C
• Gamma-trace
• MGC117328
• Neuroendocrine basic polypeptide
• Post-gamma-globulin

Background

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Cystatin C (MSCTC0)(41)

Publications using MSCTC0

• Mulla, J;Gregory, A;Liao, H;Al Matour, B;Li, Y;Abdullah, A;Billiar, TR;Scott, MJ; Caspase-11 regulates systemic inflammation and cell death in a cell-specific manner after trauma with shock Journal of leukocyte biology 2025-08-05 [PMID: 40817837] (Mouse)
• Yuan, X;Nie, S;Yang, Y;Liu, C;Xia, D;Meng, L;Xia, Y;Su, H;Zhang, C;Bu, L;Deng, M;Ye, K;Xiong, J;Chen, L;Zhang, Z; Propagation of pathologic ?-synuclein from kidney to brain may contribute to Parkinson's disease Nature neuroscience 2025-01-23 [PMID: 39849144] (Mouse)
• Tojo, K;Yazawa, T; A high fraction of inspired oxygen does not mitigate atelectasis-induced lung tissue hypoxia or injury in experimental acute respiratory distress syndrome Scientific reports 2025-01-14 [PMID: 39809828] (Rat)
• Vishwakarma, K;Bisht, A;Kumar, P;Kumar, S;Akhter, J;Payasi, A;Chaudhary, S;Aggarwal, A; Toxicokinetic Profiling of VRP-034: Evaluating its Potential in Mitigating Polymyxin B-Associated Nephrotoxicity International journal of antimicrobial agents 2024-11-27 [PMID: 39612992] (Rat)
• Kiourtis, C;Terradas-Terradas, M;Gee, LM;May, S;Georgakopoulou, A;Collins, AL;O'Sullivan, ED;Baird, DP;Hassan, M;Shaw, R;Tan, EH;Müller, M;Engelmann, C;Andreola, F;Hsieh, YC;Reed, LH;Borthwick, LA;Nixon, C;Clark, W;Hanson, PS;Sumpton, D;Mackay, G;Suzuki, T;Najumudeen, AK;Inman, GJ;Campbell, A;Barry, ST;Quaglia, A;Morris, CM;LeBeau, FEN;Sansom, OJ;Kirschner, K;Jalan, R;Oakley, F;Bird, TG; Hepatocellular senescence induces multi-organ senescence and dysfunction via TGF? Nature cell biology 2024-11-13 [PMID: 39537753] (Mouse, Transgenic Mouse)
• Mansoure, AN;Elshal, M;Helal, MG; Inhibitory effect of diacerein on diclofenac-induced acute nephrotoxicity in rats via modulating SIRT1/HIF-1?/NF-?B and SIRT1/p53 regulatory axes International immunopharmacology 2024-03-11 [PMID: 38471363] (Rat)
• Patel, MM;Gerakopoulos, V;Petsouki, E;Zimmerman, KA;Tsiokas, L; Nephronophthisis-associated FBW7 mediates cyst-dependent decline of renal function in ADPKD bioRxiv : the preprint server for biology 2024-03-02 [PMID: 38464230] (Mouse)
• Rauckhorst, AJ;Vasquez Martinez, G;Mayoral Andrade, G;Wen, H;Kim, JY;Simoni, A;Robles-Planells, C;Mapuskar, KA;Rastogi, P;Steinbach, EJ;McCormick, ML;Allen, BG;Pabla, NS;Jackson, AR;Coleman, MC;Spitz, DR;Taylor, EB;Zepeda-Orozco, D; Tubular mitochondrial pyruvate carrier disruption elicits redox adaptations that protect from acute kidney injury Molecular metabolism 2023-12-07 [PMID: 38056691] (Mouse)
• Rega, LR;Janssens, V;Graversen, JH;Moestrup, SK;Cairoli, S;Goffredo, BM;Nevo, N;Courtoy, GE;Jouret, F;Antignac, C;Emma, F;Pierreux, CE;Courtoy, PJ; Dietary supplementation of cystinotic mice by lysine inhibits the megalin pathway and decreases kidney cystine content Scientific reports 2023-10-12 [PMID: 37828038] (Mouse)
• Han, DS;Erickson, C;Hansen, KC;Kirkbride-Romeo, L;He, Z;Rodell, CB;Soranno, DE; Mesenchymal Stem Cells Delivered Locally to Ischemia-Reperfused Kidneys via Injectable Hyaluronic Acid Hydrogels Decrease Extracellular Matrix Remodeling 1 Month after Injury in Male Mice Cells 2023-07-04 [PMID: 37443806] (Mouse)
• AJ Rauckhorst, GV Martinez, GM Andrade, H Wen, JY Kim, A Simoni, KA Mapuskar, P Rastogi, EJ Steinbach, ML McCormick, BG Allen, NS Pabla, AR Jackson, MC Coleman, DR Spitz, EB Taylor, D Zepeda-Oro Tubular Mitochondrial Pyruvate Carrier Disruption Elicits Redox Adaptations that Protect from Acute Kidney Injury bioRxiv : the preprint server for biology, 2023-01-31;0(0):. 2023-01-31 [PMID: 36778297] (Mouse)
• MY Chang, SH Hsu, LY Ma, LF Chou, CC Hung, YC Tian, CW Yang Effects of Suramin on Polycystic Kidney Disease in a Mouse Model of Polycystin-1 Deficiency International Journal of Molecular Sciences, 2022-07-31;23(15):. 2022-07-31 [PMID: 35955634] (Mouse)
• Y Zhu, LGPL Prata, EOW Gerdes, JME Netto, T Pirtskhala, N Giorgadze, U Tripathi, CL Inman, KO Johnson, A Xue, AK Palmer, T Chen, K Schaefer, JN Justice, AM Nambiar, N Musi, SB Kritchevsk, J Chen, S Khosla, D Jurk, MJ Schafer, T Tchkonia, JL Kirkland Orally-active, clinically-translatable senolytics restore alpha-Klotho in mice and humans EBioMedicine, 2022-03-13;0(0):103912. 2022-03-13 [PMID: 35292270] (Mouse)
• R Harwood, J Bridge, L Ressel, L Scarfe, J Sharkey, G Czanner, PA Kalra, A Odudu, S Kenny, B Wilm, P Murray Murine models of renal ischemia reperfusion injury: An opportunity for refinement using noninvasive monitoring methods Physiological Reports, 2022-03-01;10(5):e15211. 2022-03-01 [PMID: 35266337] (Mouse)
• MJ Uddin, J Jeong, ES Pak, H Ha CO-Releasing Molecule-2 Prevents Acute Kidney Injury through Suppression of ROS-Fyn-ER Stress Signaling in Mouse Model Oxidative Medicine and Cellular Longevity, 2021-07-06;2021(0):9947772. 2021-07-06 [PMID: 34326922] (Mouse)
• ES Pak, MJ Uddin, H Ha Inhibition of Src Family Kinases Ameliorates LPS-Induced Acute Kidney Injury and Mitochondrial Dysfunction in Mice Int J Mol Sci, 2020-11-03;21(21):. 2020-11-03 [PMID: 33153232] (Mouse)
• ME Abouelela, MAA Orabi, RA Abdelhamid, MS Abdelkader, HR Madkor, FMM Darwish, T Hatano, BEM Elsadek Ethyl acetate extract of Ceiba pentandra (L.) Gaertn. reduces methotrexate-induced renal damage in rats via antioxidant, anti-inflammatory, and antiapoptotic actions Journal of traditional and complementary medicine, 2019-08-28;10(5):478-486. 2019-08-28 [PMID: 32953564] (Rat)
• K Kohl, E Herzog, G Dickneite, S Pestel Evaluation of urinary biomarkers for early detection of acute kidney injury in a rat nephropathy model J Pharmacol Toxicol Methods, 2020-08-07;0(0):106901. 2020-08-07 [PMID: 32771565] (Rat)
• Y Mizunuma, K Kanasaki, K Nitta, Y Nakamura, Y Ishigaki, Y Takagaki, M Kitada, S Li, H Liu, J Li, I Usui, Y Aso, D Koya CD-1db/db mice: a novel type 2 diabetic mouse model with progressive kidney fibrosis J Diabetes Investig, 2020-07-03;0(0):. 2020-07-03 [PMID: 32472621] (Mouse)
• S Kashyap, KZ Hein, CC Chini, J Lika, GM Warner, LK Bale, VE Torres, PC Harris, C Oxvig, CA Conover, EN Chini Metalloproteinase PAPP-A regulation of IGF-1 contributes to polycystic kidney disease pathogenesis JCI Insight, 2020-02-27;0(0):. 2020-02-27 [PMID: 31990681] (Mouse)
• MM Perales-Qu, AL Saucedo, JR Lucio-Guti, N Waksman, G Alarcon-Ga, G Govea-Torr, C Sanchez-Ma, E Pérez-Rodr, FJ Guzman-de, P Cordero-Pé Metabolomic and biochemical characterization of a new model of the transition of acute kidney injury to chronic kidney disease induced by folic acid PeerJ, 2019-06-21;7(0):e7113. 2019-06-21 [PMID: 31275747] (Rat)
• CL Lin, YC Hsu, YT Huang, YH Shih, CJ Wang, WC Chiang, PJ Chang A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction EMBO Mol Med, 2019-05-01;11(5):. 2019-05-01 [PMID: 30948420] (Mouse)
• SP Srivastava, J Li, M Kitada, H Fujita, Y Yamada, JE Goodwin, K Kanasaki, D Koya SIRT3 deficiency leads to induction of abnormal glycolysis in diabetic kidney with fibrosis Cell Death Dis, 2018-09-24;9(10):997. 2018-09-24 [PMID: 30250024] (Mouse)
• K Czarzasta, A Cudnoch-Je, L Niemczyk, R Wrzesien, M Tkaczyk, L Puchalska, M Saracyn, W Zmudzki, S Niemczyk Effect of Chronic Kidney Disease on Changes in Vasopressin System Expression in the Kidney Cortex in Rats with Nephrectomy Biomed Res Int, 2018-06-14;2018(0):2607928. 2018-06-14 [PMID: 30013980] (Rat)
• MKE Ougaard, PH Kvist, HE Jensen, C Hess, I Rune, H Søndergaar Murine Nephrotoxic Nephritis as a Model of Chronic Kidney Disease Int J Nephrol, 2018-03-05;2018(0):8424502. 2018-03-05 [PMID: 29692933] (Mouse)
• K Yoshikawa, M Iwasa, A Eguchi, S Kojima, N Yoshizawa, M Tempaku, R Sugimoto, N Yamamoto, K Sugimoto, Y Kobayashi, H Hasegawa, Y Takei Neutrophil gelatinase-associated lipocalin level is a prognostic factor for survival in rat and human chronic liver diseases Hepatol Commun, 2017-10-16;1(9):946-956. 2017-10-16 [PMID: 29404502] (ELISA Capture, Rat)
• A Bienholz, R Mae Pang, H Guberina, U Rauen, O Witzke, B Wilde, F Petrat, T Feldkamp, A Kribben Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat Model Kidney Blood Press. Res., 2017-12-05;42(6):1090-1103. 2017-12-05 [PMID: 29207388] (Rat)
• S Tsuji, M Sugiura, S Tsutsumi, H Yamada Sex differences in the excretion levels of traditional and novel urinary biomarkers of nephrotoxicity in rats J Toxicol Sci, 2017-01-01;42(5):615-627. 2017-01-01 [PMID: 28904297] (Rat)
• SL Walton, H Bielefeldt, RR Singh, J Li, TM Paravicini, MH Little, KM Moritz Prenatal hypoxia leads to hypertension, renal renin-angiotensin system activation and exacerbates salt-induced pathology in a sex-specific manner Sci Rep, 2017-08-15;7(1):8241. 2017-08-15 [PMID: 28811528] (Mouse)
• D Jensen, C Kierulf-La, MLV Kristensen, R Nørregaard, K Weyer, R Nielsen, EI Christense, H Birn Megalin dependent urinary cystatin C excretion in ischemic kidney injury in rats PLoS ONE, 2017-06-02;12(6):e0178796. 2017-06-02 [PMID: 28575050] (Mouse, Rat)
• ME Turner, KJ Laverty, PS Jeronimo, M Kaufmann, G Jones, CA White, RM Holden, MA Adams Validation of a routine two-sample iohexol plasma clearance assessment of GFR and an evaluation of common endogenous markers in a rat model of CKD Physiol Rep, 2017-05-01;5(9):. 2017-05-01 [PMID: 28483858] (Rat)
• TJ Pianta, JW Pickering, L Succar, M Chin, T Davidson, NA Buckley, F Mohamed, ZH Endre Dexamethasone Modifies Cystatin C-Based Diagnosis of Acute Kidney Injury During Cisplatin-Based Chemotherapy Kidney Blood Press. Res, 2017-03-17;42(1):62-75. 2017-03-17 [PMID: 28315878] (Rat)
• R Watanabe, JI Suzuki, K Wakayama, Y Maejima, M Shimamura, H Koriyama, H Nakagami, H Kumagai, Y Ikeda, H Akazawa, R Morishita, I Komuro, M Isobe A peptide vaccine targeting angiotensin II attenuates the cardiac dysfunction induced by myocardial infarction Sci Rep, 2017-03-07;7(0):43920. 2017-03-07 [PMID: 28266578] (Rat)
• Obesity-induced kidney injury is attenuated by amelioration of aberrant PHD2 activation in proximal tubules Sci Rep, 2016-11-09;6(0):36533. 2016-11-09 [PMID: 27827416] (Mouse)
• Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis Respir Res, 2016-09-22;17(1):118. 2016-09-22 [PMID: 27658724] (Mouse)
• Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis Sci Rep, 2016-07-18;6(0):29884. 2016-07-18 [PMID: 27425816] (Mouse)
• DH Kim, BH Choi, SK Ku, JH Park, E Oh, MK Kwak Beneficial Effects of Sarpogrelate and Rosuvastatin in High Fat Diet/Streptozotocin-Induced Nephropathy in Mice PLoS ONE, 2016-04-20;11(4):e0153965. 2016-04-20 [PMID: 27097221] (Mouse)
• Nozako M, Koyama T, Nagano C, Sato M, Matsumoto S, Mitani K, Yasufuku R, Kohashi M, Yoshikawa T An Atherogenic Paigen-Diet Aggravates Nephropathy in Type 2 Diabetic OLETF Rats. PLoS ONE, 2015-11-25;10(11):e0143979. 2015-11-25 [PMID: 26606054] (Rat)
• Wu J, Cheng Z, Gu Y, Zou W, Zhang M, Zhu P, Hu S Aggravated Cardiac Remodeling post Aortocaval Fistula in Unilateral Nephrectomized Rats. PLoS ONE, 2015-08-07;10(8):e0134579. 2015-08-07 [PMID: 26252578] (Rat)
• Shi S, Srivastava S, Kanasaki M, He J, Kitada M, Nagai T, Nitta K, Takagi S, Kanasaki K, Koya D Interactions of DPP-4 and integrin beta1 influences endothelial-to-mesenchymal transition. Kidney Int, 2015-04-01;88(3):479-89. 2015-04-01 [PMID: 25830763] (Mouse)
• Kong H, Chen F, He Y, Wu L, Wang L, Zhu S, Zheng S Intrarenal resistance index for the assessment of acute renal injury in a rat liver transplantation model. BMC Nephrol, 2013-03-02;14(0):55. 2013-03-02 [PMID: 23453043] (Rat)

FAQs for Cystatin C (MSCTC0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Cst3