alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse pancreas. NAGLU antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Rat duodenum. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Mouse brain. NAGLU antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU protein at cytoplasm by immunofluorescent analysis.Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha-N-acetylglucosaminidase/NAGLU protein stained by alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:250.Blue: Hoechst 33342 staining.

alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Green: alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) .Blue: Nuclear staining with Hoechst 33342. ^[^GTX]br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha-N-acetylglucosaminidase/NAGLU antibody detects alpha-N-acetylglucosaminidase/NAGLU Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: alpha-N-acetylglucosaminidase/NAGLU stained by alpha-N-acetylglucosaminidase/NAGLU antibody (NBP1-31555) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free (NBP1-31555) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha-N-acetylglucosaminidase/NAGLU. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAGLU
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

• alphaNacetylglucosaminidase
• alpha-N-acetylglucosaminidase
• EC 3.2.1.50
• MPS3B
• MPS-IIIB
• N-acetyl-alpha-glucosaminidase
• N-acetylglucosaminidase, alpha
• NAG
• NAGalpha-N-acetylglucosaminidase
• NAGLU
• UFHSD
• UFHSD1

Background

This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAGLU
• Entrez:
  • 4669(Human)
• Uniprot:
  • P54802()