Mouse Lipocalin-2/NGAL DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Lipocalin-2/NGAL DuoSet ELISA, 15 Plate Summary

• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Lcn2

Applications/Dilutions

• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Lipocalin-2/NGAL DuoSet ELISA, 15 Plate

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Publications for Lipocalin-2/NGAL (DY1857)(55)

Publications using DY1857

• M Biagioli, A Carino, C Di Giorgio, S Marchianò, M Bordoni, R Roselli, E Distrutti, S Fiorucci Discovery of a Novel Multi-Strains Probiotic Formulation with Improved Efficacy toward Intestinal Inflammation Nutrients, 2020;12(7):. 2020 [PMID: 32629887] (Mouse)
• P Tod, EN Bukosza, B Róka, T Kaucsár, A Fintha, T Krenács, G Szénási, P Hamar Post-Ischemic Renal Fibrosis Progression Is Halted by Delayed Contralateral Nephrectomy: The Involvement of Macrophage Activation Int J Mol Sci, 2020;21(11):. 2020 [PMID: 32481551] (Mouse)
• E van Tilbur, VK Pettersen, MW Gutierrez, I Laforest-L, NG Jendzjowsk, JB Cavin, FA Vicentini, CM Keenan, HR Ramay, J Samara, WK MacNaughto, RJA Wilson, MM Kelly, KD McCoy, KA Sharkey, MC Arrieta Intestinal fungi are causally implicated in microbiome assembly and immune development in mice Nat Commun, 2020;11(1):2577. 2020 [PMID: 32444671] (Mouse)
• P Saha, X Xiao, Y Li, RM Golonka, AA Abokor, BS Yeoh, M Vijay-Kuma Distinct iron homeostasis in C57BL/6 and Balb/c mouse strains Physiol Rep, 2020;8(9):e14441. 2020 [PMID: 32385968] (Mouse)
• AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020;31(1):107471. 2020 [PMID: 32268087] (Mouse)
• M Kumar, A Leon Coria, S Cornick, B Petri, S Mayengbam, HB Jijon, F Moreau, J Shearer, K Chadee Increased intestinal permeability exacerbates sepsis through reduced hepatic SCD-1 activity and dysregulated iron recycling Nat Commun, 2020;11(1):483. 2020 [PMID: 31980623] (Mouse)
• BJ Bivona, S Takai, DM Seth, R Satou, LM Harrison-B Chymase inhibition retards albuminuria in type 2 diabetes Physiol Rep, 2019;7(24):e14302. 2019 [PMID: 31872559] (Mouse)
• HQ Tran, RE Ley, AT Gewirtz, B Chassaing Flagellin-elicited adaptive immunity suppresses flagellated microbiota and vaccinates against chronic inflammatory diseases Nat Commun, 2019;10(1):5650. 2019 [PMID: 31827095] (Mouse)
• DT Riglar, DL Richmond, L Potvin-Tro, AA Verdegaal, AD Naydich, S Bakshi, E Leoncini, LG Lyon, J Paulsson, PA Silver Bacterial variability in the mammalian gut captured by a single-cell synthetic oscillator Nat Commun, 2019;10(1):4665. 2019 [PMID: 31604953] (Mouse)
• A Suwandi, A Galeev, R Riedel, S Sharma, K Seeger, T Sterzenbac, L García Pas, EC Boyle, O Gal-Mor, M Hensel, J Casadesús, JF Baines, GA Grassl Std fimbriae-fucose interaction increases Salmonella-induced intestinal inflammation and prolongs colonization PLoS Pathog., 2019;15(7):e1007915. 2019 [PMID: 31329635] (Mouse)
• KJ Meade, F Sanchez, A Aguayo, N Nadales, SG Hamalian, TL Uhlendorf, LR Banner, JA Kelber Secretomes from metastatic breast cancer cells, enriched for a prognostically unfavorable LCN2 axis, induce anti-inflammatory MSC actions and a tumor-supportive premetastatic lung Oncotarget, 2019;10(32):3027-3039. 2019 [PMID: 31105883] (Mouse)
• H Dubois, F Sorgeloos, ST Sarvestani, L Martens, Y Saeys, JM Mackenzie, M Lamkanfi, G van Loo, I Goodfellow, A Wullaert Nlrp3 inflammasome activation and Gasdermin D-driven pyroptosis are immunopathogenic upon gastrointestinal norovirus infection PLoS Pathog., 2019;15(4):e1007709. 2019 [PMID: 31017981] (Mouse)
• L Zhou, C Chu, F Teng, NJ Bessman, J Goc, EK Santosa, GG Putzel, H Kabata, JR Kelsen, RN Baldassano, MA Shah, RE Sockolow, E Vivier, G Eberl, KA Smith, GF Sonnenberg Innate lymphoid cells support regulatory T cells in the intestine through interleukin-2 Nature, 2019;0(0):. 2019 [PMID: 30944470] (Mouse)
• EGD Hopkins, TI Roumelioti, C Mullineaux, JS Choudhary, G Frankel Intestinal Epithelial Cells and the Microbiome Undergo Swift Reprogramming at the Inception of Colonic Citrobacter rodentium Infection MBio, 2019;10(2):. 2019 [PMID: 30940698] (Mouse)
• JL Pope, Y Yang, RC Newsome, W Sun, X Sun, M Ukhanova, J Neu, JP Issa, V Mai, C Jobin Microbial Colonization Coordinates the Pathogenesis of a Klebsiella pneumoniae Infant Isolate Sci Rep, 2019;9(1):3380. 2019 [PMID: 30833613] (Mouse)
• F Laudisi, D Di Fusco, V Dinallo, C Stolfi, A Di Grazia, I Marafini, A Colantoni, A Ortenzi, C Alteri, F Guerrieri, M Mavilio, F Ceccherini, M Federici, TT MacDonald, I Monteleone, G Monteleone The Food Additive Maltodextrin Promotes Endoplasmic Reticulum Stress-Driven Mucus Depletion and Exacerbates Intestinal Inflammation Cell Mol Gastroenterol Hepatol, 2019;7(2):457-473. 2019 [PMID: 30765332] (Mouse)
• P Saha, BS Yeoh, X Xiao, RM Golonka, V Singh, Y Wang, M Vijay-Kuma PAD4-dependent NETs generation are indispensable for intestinal clearance of Citrobacter rodentium Mucosal Immunol, 2019;0(0):. 2019 [PMID: 30710097] (Mouse)
• C Janowitz, YK Nakamura, C Metea, A Gligor, W Yu, L Karstens, JT Rosenbaum, M Asquith, P Lin Disruption of Intestinal Homeostasis and Intestinal Microbiota During Experimental Autoimmune Uveitis Invest. Ophthalmol. Vis. Sci., 2019;60(1):420-429. 2019 [PMID: 30695094] (Mouse)
• CN Berger, VF Crepin, TI Roumelioti, JC Wright, N Serafini, M Pevsner-Fi, L Yu, E Elinav, JP Di Santo, JS Choudhary, G Frankel The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and antimicrobial responses PLoS Pathog., 2018;14(10):e1007406. 2018 [PMID: 30365535] (Mouse)
• X Xiao, P Saha, BS Yeoh, JA Hipp, V Singh, M Vijay-Kuma Myeloperoxidase deficiency attenuates systemic and dietary iron-induced adverse effects J. Nutr. Biochem., 2018;62(0):28-34. 2018 [PMID: 30218980] (Mouse)
• JA Smith, A Braga, J Verheyen, S Basilico, S Bandiera, C Alfaro-Cer, L Peruzzotti, D Shu, F Haque, P Guo, S Pluchino RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity Mol Ther Nucleic Acids, 2018;10(0):103-121. 2018 [PMID: 29499926] (Mouse)
• HT Groves, L Cuthbertso, P James, MF Moffatt, MJ Cox, JS Tregoning Respiratory Disease following Viral Lung Infection Alters the Murine Gut Microbiota Front Immunol, 2018;9(0):182. 2018 [PMID: 29483910] (Mouse)
• DN Bronner, F Faber, EE Olsan, MX Byndloss, NA Sayed, G Xu, W Yoo, D Kim, S Ryu, CB Lebrilla, AJ Bäumler Genetic Ablation of Butyrate Utilization Attenuates Gastrointestinal Salmonella Disease Cell Host Microbe, 2018;23(2):266-273.e4. 2018 [PMID: 29447698] (Mouse)
• AF Schulfer, T Battaglia, Y Alvarez, L Bijnens, VE Ruiz, M Ho, S Robinson, T Ward, LM Cox, AB Rogers, D Knights, RB Sartor, MJ Blaser Intergenerational transfer of antibiotic-perturbed microbiota enhances colitis in susceptible mice Nat Microbiol, 2017;0(0):. 2017 [PMID: 29180726] (Mouse)
• LF Mager, VH Koelzer, R Stuber, L Thoo, I Keller, I Koeck, M Langenegge, C Simillion, SP Pfister, M Faderl, V Genitsch, I Tcymbarevi, P Juillerat, X Li, Y Xia, E Karamitopo, R Lyck, I Zlobec, S Hapfelmeie, R Bruggmann, KD McCoy, AJ Macpherson, C Müller, B Beutler, P Krebs The ESRP1-GPR137 axis contributes to intestinal pathogenesis Elife, 2017;6(0):. 2017 [PMID: 28975893] (Mouse)
• Y Kim, AG Wu, A Jaja-Chime, BL Graf, C Waterman, MP Verzi, I Raskin Isothiocyanate-enriched moringa seed extract alleviates ulcerative colitis symptoms in mice PLoS ONE, 2017;12(9):e0184709. 2017 [PMID: 28922365] (Mouse)
• K Watanabe, CA Gilchrist, MJ Uddin, SL Burgess, MM Abhyankar, SN Moonah, Z Noor, JR Donowitz, BN Schneider, T Arju, E Ahmed, M Kabir, M Alam, R Haque, P Pramoonjag, B Mehrad, WA Petri Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis PLoS Pathog., 2017;13(8):e1006513. 2017 [PMID: 28817707] (Mouse)
• M Quiros, H Nishio, PA Neumann, D Siuda, JC Brazil, V Azcutia, R Hilgarth, MN O'Leary, V Garcia-Her, G Leoni, M Feng, G Bernal, H Williams, PH Dedhia, C Gerner-Smi, J Spence, CA Parkos, TL Denning, A Nusrat Macrophage-derived IL-10 mediates mucosal repair by epithelial WISP-1 signaling J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28783045] (Mouse)
• AL Tubbs, B Liu, TD Rogers, RB Sartor, EA Miao Dietary Salt Exacerbates Experimental Colitis J. Immunol., 2017;0(0):. 2017 [PMID: 28637899] (Mouse)
• DT Riglar, TW Giessen, M Baym, SJ Kerns, MJ Niederhube, RT Bronson, JW Kotula, GK Gerber, JC Way, PA Silver Engineered bacteria can function in the mammalian gut long-term as live diagnostics of inflammation Nat. Biotechnol., 2017;0(0):. 2017 [PMID: 28553941] (Mouse)
• MA Borton, A Sabag-Daig, J Wu, LM Solden, BS O'Banion, RA Daly, RA Wolfe, JF Gonzalez, VH Wysocki, BMM Ahmer, KC Wrighton Chemical and pathogen-induced inflammation disrupt the murine intestinal microbiome Microbiome, 2017;5(1):47. 2017 [PMID: 28449706] (Mouse)
• P Saha, BS Yeoh, RA Olvera, X Xiao, V Singh, D Awasthi, BC Subramania, Q Chen, M Dikshit, Y Wang, CA Parent, M Vijay-Kuma Bacterial Siderophores Hijack Neutrophil Functions J. Immunol., 2017;0(0):. 2017 [PMID: 28432145] (Mouse)
• C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells Cell Death Dis, 2017;8(4):e2735. 2017 [PMID: 28383552] (Mouse)
• François Reichardt Western diet induces colonic nitrergic myenteric neuropathy and dysmotility in mice via saturated fatty acid- and LPS-induced TLR4 signaling J. Physiol. (Lond.), 2016;0(0):. 2016 [PMID: 28000223] (Mouse)
• Combination Therapy for Ulcerative Colitis: Orally Targeted Nanoparticles Prevent Mucosal Damage and Relieve Inflammation Theranostics, 2016;6(12):2250-2266. 2016 [PMID: 27924161] (Mouse)
• Microbiota-inducible Innate Immune, Siderophore Binding Protein Lipocalin 2 is Critical for Intestinal Homeostasis Cell Mol Gastroenterol Hepatol, 2016;2(4):482-498.e6. 2016 [PMID: 27458605] (Mouse)
• Pathogenic Upregulation of Glial Lipocalin-2 in the Parkinsonian Dopaminergic System J Neurosci, 2016;36(20):5608-22. 2016 [PMID: 27194339] (Mouse)
• Therapeutic Blockade of Immune Complex-Mediated Glomerulonephritis by Highly Selective Inhibition of Bruton's Tyrosine Kinase Sci Rep, 2016;6(0):26164. 2016 [PMID: 27192942] (Mouse)
• Kaucsar T, Godo M, Revesz C, Kovacs M, Mocsai A, Kiss N, Albert M, Krenacs T, Szenasi G, Hamar P Urine/Plasma Neutrophil Gelatinase Associated Lipocalin Ratio Is a Sensitive and Specific Marker of Subclinical Acute Kidney Injury in Mice. PLoS ONE, 2016;11(1):e0148043. 2016 [PMID: 26824608] (Mouse)
• Allais L, Kumar S, Debusschere K, Verschuere S, Maes T, De Smet R, Conickx G, De Vos M, Laukens D, Joos G, Brusselle G, Elewaut D, Cuvelier C, Bracke K The Effect of Cigarette Smoke Exposure on the Development of Inflammation in Lungs, Gut and Joints of TNFDeltaARE Mice. PLoS ONE, 2015;10(11):e0141570. 2015 [PMID: 26523550] (Mouse)
• Kortman G, Mulder M, Richters T, Shanmugam N, Trebicka E, Boekhorst J, Timmerman H, Roelofs R, Wiegerinck E, Laarakkers C, Swinkels D, Bolhuis A, Cherayil B, Tjalsma H Low dietary iron intake restrains the intestinal inflammatory response and pathology of enteric infection by food-borne bacterial pathogens. Eur J Immunol, 2015;45(9):2553-67. 2015 [PMID: 26046550] (Mouse)
• Chassaing, Benoit, Koren, Omry, Goodrich, Julia K, Poole, Angela C, Srinivasan, Shanthi, Ley, Ruth E, Gewirtz, Andrew T Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome. Nature, 2015;519(7541):92-6. 2015 [PMID: 25731162] (Mouse)
• Leoni, Giovanna, Neumann, Philipp-, Kamaly, Nazila, Quiros, Miguel, Nishio, Hikaru, Jones, Hefin R, Sumagin, Ronen, Hilgarth, Roland S, Alam, Ashfaqul, Fredman, Gabriell, Argyris, Ioannis, Rijcken, Emile, Kusters, Dennis, Reutelingsperger, Chris, Perretti, Mauro, Parkos, Charles, Farokhzad, Omid C, Neish, Andrew S, Nusrat, Asma Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. J Clin Invest, 2015;125(3):1215-27. 2015 [PMID: 25664854] (Mouse)
• Floderer M, Prchal-Murphy M, Vizzardelli C Dendritic cell-secreted lipocalin2 induces CD8+ T-cell apoptosis, contributes to T-cell priming and leads to a TH1 phenotype. PLoS ONE, 2014;9(7):e101881. 2014 [PMID: 25010215] (Mouse)
• Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner. Infect Immun, 2014;82(9):3723-39. 2014 [PMID: 24958709] (Mouse)
• Zager R, Johnson A, Frostad K Rapid renal alpha-1 antitrypsin gene induction in experimental and clinical acute kidney injury. PLoS ONE, 2014;9(5):e98380. 2014 [PMID: 24848503] (Mouse)
• Shashidharamurthy R, Machiah D, Aitken J, Putty K, Srinivasan G, Chassaing B, Parkos C, Selvaraj P, Vijay-Kumar M Differential role of lipocalin 2 during immune complex-mediated acute and chronic inflammation in mice. Arthritis Rheum, 2013;65(4):1064-73. 2013 [PMID: 23280250] (Mouse)
• Fecal lipocalin 2, a sensitive and broadly dynamic non-invasive biomarker for intestinal inflammation. PLoS ONE, 2012;7(9):e44328. 2012 [PMID: 22957064] (Mouse)
• Chassaing B, Srinivasan G, Delgado M, Young A, Gewirtz A, Vijay-Kumar M Fecal lipocalin 2, a sensitive and broadly dynamic non-invasive biomarker for intestinal inflammation. PLoS ONE, 2012;7(9):e44328. 2012 [PMID: 22957064] (Mouse)
• Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 2012;7(0):87-104. 2012 [PMID: 22837640] (Mouse)
• Ganguly K, Upadhyay S, Irmler M, Takenaka S, Pukelsheim K, Beckers J, de Angelis MH, Hamelmann E, Stoeger T, Schulz H Impaired resolution of inflammatory response in the lungs of JF1/Msf mice following carbon nanoparticle instillation. Respir. Res., 2011;12(0):94. 2011 [PMID: 21756372] (Mouse)
• Pitteri SJ, Kelly-Spratt KS, Gurley KE Tumor microenvironment-derived proteins dominate the plasma proteome response during breast cancer induction and progression. Cancer Res., 2011;71(15):5090-100. 2011 [PMID: 21653680] (Mouse)
• Kelly-Spratt KS, Pitteri SJ, Gurley KE, Liggitt D, Chin A, Kennedy J, Wong CH, Zhang Q, Buson TB, Wang H, Hanash SM, Kemp CJ Plasma proteome profiles associated with inflammation, angiogenesis, and cancer. PLoS ONE, 2011;6(5):e19721. 2011 [PMID: 21589862] (Mouse)
• Vijay-Kumar M, Aitken JD, Carvalho FA, Cullender TC, Mwangi S, Srinivasan S, Sitaraman SV, Knight R, Ley RE, Gewirtz AT Metabolic syndrome and altered gut microbiota in mice lacking Toll-like receptor 5. Science, 2010;328(5975):228-31. 2010 [PMID: 20203013] (Mouse)
• Pitteri SJ, JeBailey L, Faca VM, Thorpe JD, Silva MA, Ireton RC, Horton MB, Wang H, Pruitt LC, Zhang Q, Cheng KH, Urban N, Hanash SM, Dinulescu DM Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery. PLoS ONE, 2009;4(11):e7916. 2009 [PMID: 19936259] (Mouse)

FAQs for Lipocalin-2/NGAL (DY1857). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: Lcn2