TIM-4 Antibody [Unconjugated]

Images

 
Western blot shows 25 ng of Recombinant Human TIM‑4 His-tag (Catalog # 2929-TM), Recombinant Human TIM‑1/KIM‑1/HAVCR (Catalog # 1750-TM), Recombinant Human TIM‑3 Fc Chimera (Catalog # 2365-TM), ...read more
TIM‑4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human TIM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2929) at 1 µg/mL for 1 hour at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

TIM-4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM-4 (R&D Systems, Catalog # 2929-TM)
Glu25-Leu315
Accession # Q96H15
Specificity
Detects human TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) TIM-1 and recombinant mouse (rm) TIM-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIMD4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 1-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2929 in the following applications:

Publications
Read Publications using
AF2929 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-4 Antibody [Unconjugated]

  • FLJ27515
  • Smuckler
  • T-cell immunoglobulin and mucin domain containing 4
  • T-cell immunoglobulin and mucin domain-containing protein 4
  • T-cell membrane protein 4
  • TIM4
  • TIM-4
  • TIMD4
  • TIMD-4

Background

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells (5).

  1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681. 
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. 
  4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
  5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-76701
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, KD
NB100-40853
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-23000
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-31470
Species: Hu, Mu, Rt, Bv, V-Vi
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF2365
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Dual ISH-IHC
NBP1-71693
Species: Hu
Applications: WB, ICC/IF
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
6507-IL/CF
Species: Hu
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF904
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, Neut
NB100-56722
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, DB, ELISA, Flow, Func, ICC/IF, IP, In vitro, B/N, Flow-CS
NB100-56566
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF2929
Species: Hu
Applications: WB, IHC

Publications for TIM-4 Antibody (AF2929)(3)

Review for TIM-4 Antibody (AF2929) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using AF2929:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Other 01/07/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 23084921
SpeciesOther

Blocking

Blocking DetailsSee PMID 23084921

Details

Detection NotesSee PMID 23084921

Comments

CommentsPublished: See PMID 23084921

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TIM-4 Antibody (AF2929) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2929

Additional TIM-4 Products

Bioinformatics Tool for TIM-4 Antibody (AF2929)

Discover related pathways, diseases and genes to TIM-4 Antibody (AF2929). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TIM-4 Antibody (AF2929)

Discover more about diseases related to TIM-4 Antibody (AF2929).
 

Pathways for TIM-4 Antibody (AF2929)

View related products by pathway.

PTMs for TIM-4 Antibody (AF2929)

Learn more about PTMs related to TIM-4 Antibody (AF2929).

Blogs on TIM-4

There are no specific blogs for TIM-4, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
01/07/2015
Application: Flow
Species: Other

Bioinformatics

Gene Symbol TIMD4
Uniprot