PER1 Antibody - BSA Free

Western Blot: PER1 Antibody [NBP2-24589] - Analysis of PER1 in HEPG2 cell lysates in the 1) absence and 2) presence of immunizing peptide using NBP2-24589. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: PER1 Antibody [NBP2-24589] - Human brain tissue using an isotype control (top) and NBP2-24589 (bottom) at 5 ug/ml.

• Reactivity: Hu, Mu, Bv, Ca, Eq, Pm, Rt
• Applications: WB, Simple Western, IHC, WB, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PER1 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit PER1 Antibody - BSA Free (NBP2-24589) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-PER1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 1150-1200 of human PER1 was used as the immunogen.
• Predicted Species: Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PER1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting reported in scientific literature (PMID 29937374)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Knockout Validated reported in scientific literature (Hamilton et al)
  • Simple Western 1:1000 - 1:5000
  • Western Blot 5-10 ug/ml
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in C57BL/6 liver whole-cell lysate (CT15), separated by Size, antibody dilution of 1:1000, 1:2000, 1:5000, apparent MW was 146 kDa

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29937374).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PER1 Antibody - BSA Free

• Circadian clock protein PERIOD 1
• Circadian pacemaker protein Rigui
• hPER1
• KIAA0482
• PER1
• PERhPER
• period (Drosophila) homolog 1
• period circadian protein homolog 1
• period homolog 1 (Drosophila)
• Period, drosophila, homolog of
• PERIOD1
• RIGUI
• RIGUIMGC88021

Background

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PER1 Antibody (NBP2-24589)(4)

Publications using NBP2-24589

• Singh G Role of Glucose-induced Transcription Factor Signalling and Mitochondrial Epigenetics in Stress Tolerant Wood Frog, Rana sylvatica Thesis 2022-12-16 (Western Blot, Amphibian)
  
  Details:
  Wood Frog
• Colleen M. Bartman, Lisa Nesbitt, Kenge K. Lee, Latifa Khalfaoui, Yun‐Hua Fang, Christina M. Pabelick, Y. S. Prakash BMAL1 sex‐specific effects in the neonatal mouse airway exposed to moderate hyperoxia Physiological Reports 2024-06-28 [PMID: 38942729]
• Toledo M, Batista-Gonzalez A, Merheb E et al. Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY2 Cell Metab. 2018-08-07 [PMID: 29937374] (IB, Mouse)
• Hamilton K. Basic Helix Loop Helix Enhancer 40 in Neuronal Excitability and Synaptic Plasticity Dissertation 2016-01-01 (KO, WB, Mouse)

Reviews for PER1 Antibody (NBP2-24589) (2)

Average Rating: 1.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Other.

reviewed by: Verified Customer IHC-Fr Other 09/11/2023

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: brain section
• Species: Other
• Lot: 03253611A-2

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

reviewed by: Katharina Sahiri WB Human 02/21/2019

Summary

• Application: Western Blot
• Sample Tested: U-2 OS cells
• Species: Human
• Lot: 03253611A

Comments

• Comments: unspecific bands with no obvious band of correct size

Bioinformatics

• Gene Symbol: PER1