TIM-3 Antibody [Unconjugated]

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TIM‑3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human TIM‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2365) at 3 µg/mL for 1 hour at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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TIM-3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM‑3
Ser22-Arg200
Accession # Q8TDQ0
Specificity
Detects human TIM-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM‑3 is observed and less than 1% cross-reactivity with recombinant human TIM-1, rmTIM-1, and rmTIM-2 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HAVCR2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • CyTOF-ready
Control
TIM-3 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
AF2365 in the following applications:

Publications
Read Publications using
AF2365 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-3 Antibody [Unconjugated]

  • CD366
  • FLJ14428
  • HAVCR2
  • HAVcr-2
  • hepatitis A virus cellular receptor 2
  • kidney injury molecule-3
  • KIM-3
  • SPTCL
  • T cell immunoglobulin mucin 3
  • T cell immunoglobulin mucin-3
  • T-cell immunoglobulin and mucin domain-containing protein 3
  • TIM 3
  • TIM3 T-cell membrane protein 3
  • TIM3
  • TIM-3
  • TIMD3
  • TIMD-3
  • TIMD3KIM-3

Background

TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11, 13), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (14). Soluble mouse TIM-3 constructs which lack the cytoplasmic domain have been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (5, 15).

  1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B. 360:1681.
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
  4. Monney, L. et al. (2002) Nature 415:536.
  5. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  6. Khademi, M. et al. (2004) J. Immunol. 172:7169.
  7. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  8. van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
  9. Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
  10. Oikawa, T. et al. (2006) J. Immunol. 177:4281.
  11. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  12. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  13. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
  14. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
  15. Geng, H. et al. (2006) J. Immunol. 176:1411.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIM-3 Antibody (AF2365)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(2)
ICC
(2)
IHC
(3)
IHC-P
(2)
Immunoprecipitation
(2)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(15)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF2365 Applications Species
DM Franchini, O Lanvin, M Tosolini, E Patras de, A Cammas, S Péricart, CM Scarlata, M Lebras, C Rossi, L Ligat, F Pont, PB Arimondo, C Laurent, M Ayyoub, F Despas, M Lapeyre-Me, S Millevoi, JJ Fournié Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells Cell Rep, 2019;26(1):94-107.e7. 2019 [PMID: 30605689] (Flow Cytometry, Human) Flow Cytometry Human
D Zhao, M Guo, B Liu, Q Lin, T Xie, Q Zhang, X Jia, Q Shu, X Liang, L Gao, C Ma Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28754800] (Western Blot, Human) Western Blot Human
J Zhao, B Shiratori, H Chagan-Yas, M Matsumoto, T Niki, M Tanaka, Y Takahashi, O Usami, Y Ashino, T Hattori Secretion of IFN-? Associated with Galectin-9 Production by Pleural Fluid Cells from a Patient with Extrapulmonary Tuberculosis Int J Mol Sci, 2017;18(7):. 2017 [PMID: 28657598] (IHC, Human) IHC Human
TO Mohammed, H Chagan-Yas, Y Ashino, W Nakayama, Y Takahashi, T Shimomura, T Fujimoto, Y Watanabe, T Niki, H Suzushima, T Hattori Galectin-9 as a Predictive Marker for the Onset of Immune-Related Adverse Effects Associated with Anti-CCR4 MoAb Therapy in Patients with Adult T Cell Leukemia Tohoku J. Exp. Med, 2017;241(3):201-208. 2017 [PMID: 28321034] (IHC, Human) IHC Human
JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9 J. Immunol, 2017;0(0):. 2017 [PMID: 28264968] (ICC, Human) ICC Human
Guangxiang Luo TIM-1 promotes hepatitis C virus cell attachment and infection J. Virol., 2016;0(0):. 2016 [PMID: 27807228] (Western Blot, Human) Western Blot Human
Comprehensive analyses of tumor immunity: implications for cancer immunotherapy Genome Biol, 2016;17(1):174. 2016 [PMID: 27549193] (IHC-P, Human) IHC-P Human
Clayton K, Douglas-Vail M, Nur-ur Rahman A, Medcalf K, Xie I, Chew G, Tandon R, Lanteri M, Norris P, Deeks S, Ndhlovu L, Ostrowski M Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression. J Virol, 2015;89(7):3723-36. 2015 [PMID: 25609823] (Human) Human
Moller-Tank S, Albritton L, Rennert P, Maury W Characterizing functional domains for TIM-mediated enveloped virus entry. J Virol, 2014;88(12):6702-13. 2014 [PMID: 24696470] (Flow Cytometry, Human) Flow Cytometry Human
Jiang, Jing, Jin, Mei-Shan, Kong, Fei, Cao, Donghui, Ma, Hong-Xi, Jia, Zhifang, Wang, Yin-Ping, Suo, Jian, Cao, Xueyuan Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis in gastric cancer. PLoS ONE, 2013;8(12):e81799. 2013 [PMID: 24339967] (IHC-P, Human) IHC-P Human
Show All 15 Publications.

Review for TIM-3 Antibody (AF2365) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2365:
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 TIM-3 AF2365
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reviewed by:
Anonymous
Human 05/02/2019
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Summary

Sample Testedhuman tonsil
SpeciesHuman
LotN/A

Comments

CommentspH 9 heat-induced antigen retrieval

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TIM-3 Antibody (AF2365) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2365

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Bioinformatics Tool for TIM-3 Antibody (AF2365)

Discover related pathways, diseases and genes to TIM-3 Antibody (AF2365). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on TIM-3.

TIM-3, a critical immune checkpoint in HIV research
CD4+ T-helper cells (Th) are the white blood lymphocytes expressing surface glycoprotein antigen CD4. These T-helper cells play an important role in the adaptive immune system by releasing T cell cytokines that help other immune cells to suppress o...  Read full blog post.

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Anonymous
05/02/2019
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Species: Human

Bioinformatics

Gene Symbol HAVCR2
Uniprot