TIM-3 Antibody [Unconjugated]


Orthogonal Strategies: TIM-3 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope® Probe (Catalog # 560681) and stained using ACD ...read more
TIM‑3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human TIM‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2365) at 3 µg/mL for 1 hour at room ...read more
Genetic Strategies: Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line and human TIM-3 knockout HDLM‑2 human Hodgkin’s lymphoma cell line (KO). PVDF membrane was probed with ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, Dual ISH-IHC, KO

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TIM-3 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human TIM‑3
Accession # Q8TDQ0
Detects human TIM-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM‑3 is observed and less than 1% cross-reactivity with recombinant human TIM-1, rmTIM-1, and rmTIM-2 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Dual RNAscope ISH-IHC 3-25 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Knockout Validated 1 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
AF2365 in the following applications:

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AF2365 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-3 Antibody [Unconjugated]

  • CD366
  • FLJ14428
  • HAVCR2
  • HAVcr-2
  • hepatitis A virus cellular receptor 2
  • kidney injury molecule-3
  • KIM-3
  • T cell immunoglobulin mucin 3
  • T cell immunoglobulin mucin-3
  • T-cell immunoglobulin and mucin domain-containing protein 3
  • TIM 3
  • TIM3 T-cell membrane protein 3
  • TIM3
  • TIM-3
  • TIMD3
  • TIMD-3
  • TIMD3KIM-3


TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11, 13), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (14). Soluble mouse TIM-3 constructs which lack the cytoplasmic domain have been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (5, 15).

  1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B. 360:1681.
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
  4. Monney, L. et al. (2002) Nature 415:536.
  5. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  6. Khademi, M. et al. (2004) J. Immunol. 172:7169.
  7. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
  8. van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
  9. Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
  10. Oikawa, T. et al. (2006) J. Immunol. 177:4281.
  11. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  12. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  13. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
  14. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
  15. Geng, H. et al. (2006) J. Immunol. 176:1411.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIM-3 Antibody (AF2365)(19)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.

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Flow Cytometry
Western Blot
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using AF2365 Applications Species
Saliby, RM;El Zarif, T;Bakouny, Z;Shah, V;Xie, W;Flippot, R;Denize, T;Kane, MH;Madsen, KN;Ficial, M;Hirsch, L;Wei, XX;Steinarter, JA;Harshman, LC;Vaishampayan, UN;Severgnini, M;McDermott, DF;Lee, GM;Xu, W;Van Allen, EM;McGregor, BA;Signoretti, S;Choueiri, TK;McKay, RR;Braun, DA; Circulating and intratumoral immune determinants of response to atezolizumab plus bevacizumab in patients with variant histology or sarcomatoid renal cell carcinoma Cancer immunology research 2023-06-06 [PMID: 37279009] (IHC, Human) IHC Human
M Calvet-Mir, I Sánchez-Ce, N Martín-Cóf, P Martínez-F, H de la Fuen, I Tsukalov, C Delgado-Ar, MJ Calzada, I de Los San, J Sanz, L García-Fra, F Sánchez-Ma, A Alfranca, MÁ Muñoz-Fern, MJ Buzón, E Martín-Gay Antiretroviral therapy duration and immunometabolic state determine efficacy of ex vivo dendritic cell-based treatment restoring functional HIV-specific CD8+ T cells in people living with HIV EBioMedicine, 2022-06-02;81(0):104090. 2022-06-02 [PMID: 35665682] (Neutralization, Human) Neutralization Human
W Zhong, X Liu, Z Zhu, Q Li, K Li High levels of Tim-3+Foxp3+Treg cells in the tumor microenvironment is a prognostic indicator of poor survival of diffuse large B cell lymphoma patients International immunopharmacology, 2021-04-14;96(0):107662. 2021-04-14 [PMID: 33864956] (IHC, Human) IHC Human
N Ebata, M Fujita, S Sasagawa, K Maejima, Y Okawa, Y Hatanaka, T Mitsuhashi, A Oosawa-Tat, H Tanaka, S Miyano, T Nakamura, S Hirano, H Nakagawa Molecular Classification and Tumor Microenvironment Characterization of Gallbladder Cancer by Comprehensive Genomic and Transcriptomic Analysis Cancers, 2021-02-10;13(4):. 2021-02-10 [PMID: 33578820] (IHC, Human) IHC Human
DM Franchini, O Lanvin, M Tosolini, E Patras de, A Cammas, S Péricart, CM Scarlata, M Lebras, C Rossi, L Ligat, F Pont, PB Arimondo, C Laurent, M Ayyoub, F Despas, M Lapeyre-Me, S Millevoi, JJ Fournié Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells Cell Rep, 2019-01-02;26(1):94-107.e7. 2019-01-02 [PMID: 30605689] (Flow Cytometry, Human) Flow Cytometry Human
D Zhao, M Guo, B Liu, Q Lin, T Xie, Q Zhang, X Jia, Q Shu, X Liang, L Gao, C Ma Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE J. Leukoc. Biol., 2017-07-28;0(0):. 2017-07-28 [PMID: 28754800] (Western Blot, Human) Western Blot Human
J Zhao, B Shiratori, H Chagan-Yas, M Matsumoto, T Niki, M Tanaka, Y Takahashi, O Usami, Y Ashino, T Hattori Secretion of IFN-? Associated with Galectin-9 Production by Pleural Fluid Cells from a Patient with Extrapulmonary Tuberculosis Int J Mol Sci, 2017-06-28;18(7):. 2017-06-28 [PMID: 28657598] (IHC, Human) IHC Human
TO Mohammed, H Chagan-Yas, Y Ashino, W Nakayama, Y Takahashi, T Shimomura, T Fujimoto, Y Watanabe, T Niki, H Suzushima, T Hattori Galectin-9 as a Predictive Marker for the Onset of Immune-Related Adverse Effects Associated with Anti-CCR4 MoAb Therapy in Patients with Adult T Cell Leukemia Tohoku J. Exp. Med, 2017-03-01;241(3):201-208. 2017-03-01 [PMID: 28321034] (IHC, Human) IHC Human
JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9 J. Immunol, 2017-03-06;0(0):. 2017-03-06 [PMID: 28264968] (ICC, Human) ICC Human
Guangxiang Luo TIM-1 promotes hepatitis C virus cell attachment and infection J. Virol., 2017-01-03;0(0):. 2017-01-03 [PMID: 27807228] (Western Blot, Human) Western Blot Human
Show All 19 Publications.

Reviews for TIM-3 Antibody (AF2365) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF2365:
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immunofluorescence - paraffin
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Images Ratings Applications Species Date Details
Western Blot TIM-3 AF2365
reviewed by:
Verified Customer
WB Human 04/04/2020


ApplicationWestern Blot
Sample TestedJurkat
Other TIM-3 AF2365
reviewed by:
Verified Customer
immunofluorescence - paraffin Human 05/02/2019


Sample Testedhuman tonsil


CommentspH 9 heat-induced antigen retrieval

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Blogs on TIM-3.

TIM-3, a critical immune checkpoint in HIV research
CD4+ T-helper cells (Th) are the white blood lymphocytes expressing surface glycoprotein antigen CD4. These T-helper cells play an important role in the adaptive immune system by releasing T cell cytokines that help other immune cells to suppress o...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Human

Verified Customer
Application: immunofluorescence - paraffin
Species: Human


Gene Symbol HAVCR2