TIGAR/C12orf5 Antibody

Images

 
Western Blot: TIGAR/C12orf5 Antibody [NBP1-49534] - Analysis of TIGAR in HeLa whole cell extracts.
Immunohistochemistry: TIGAR/C12orf5 Antibody [NBP1-49534] - Analysis of TIGAR in mouse muscle.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

TIGAR/C12orf5 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human TIGAR protein (within residues 50-200). [Swiss-Prot Q9NQ88]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
C12ORF5
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This TIGAR antibody is useful for Immunohistochemistry-paraffin sections and Western blot. In Western blot bands are seen ~30 kDa, representing TIGAR, and ~47 kDa. At this time we cannot explain the band at 47 kDa. This antibody does not appear to work in ICC.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49534 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for TIGAR/C12orf5 Antibody

  • C12orf5
  • chromosome 12 open reading frame 5
  • EC 3.1.3.46
  • probable fructose-2,6-bisphosphatase TIGAR
  • TIGAR
  • TIGARTP53-induced glycolysis and apoptosis regulator
  • transactivated by NS3TP2 protein

Background

TIGAR (TP53-induced glycolysis and apoptosis regulator), a member of phosphoglycerate mutase family, is a target gene of p53 and it regulates glucose metabolism in cancer cells by acting as a fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate (Fru-2,6-P2 ) and Fru-1,6-P2 leading to decreased Fru-2,6-P2 levels with subsequent reduction in PFK1 (phosphofructosekinase-1) activity as well as negative regulation of glycolysis. TIGAR also reduces cellular ROS through its translocation to the mitochondria where it interacts with HK2 leading to enhanced HK2 activity, regulation of mitochondrial membrane potential, and decreased mitochondrial ROS which ultimately protects the cells from DNA damage-induced apoptosis. Moreover, TIGAR mediated negative regulation of glycolysis leads to redirection of glycolytic metabolic intermediates towards pentose phosphate pathway's oxidative branch that follows increased cellular NADPH production, ROS scavenging by GSH and thus a lower sensitivity of cells to oxidative stress-associated apoptosis, including that induced by p53. Because of TIGAR's anti-apoptotic/protective role, it has been proposed to implicate in cell-cycle arrest and DNA repair effector response in cells that suffer from repairable dose of genotoxic/cellular insult.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIGAR/C12orf5 Antibody (NBP1-49534) (0)

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Review for TIGAR/C12orf5 Antibody (NBP1-49534) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-49534:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot TIGAR/C12orf5 NBP1-49534
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reviewed by:
Anonymous
WB Human 01/30/2012
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Summary

ApplicationWestern Blot
Sample TestedHepG2 total lysate, Sample Amount: ~ 50 ug
SpeciesHuman
CommentsWe have tested this TIGAR antibody is various human hepatocellular carcinoma cell lines. As the figure shows TIGAR is highly expressed in HepG2 cells, thus we use this cell line as positive control. Here we loaded about 50 ug, primary antibody was diluted to 1:4000 in TBS-T+5% dry milk (overnight incubation at 4 C). The antibody picks up 3 non-specific bands between as the picture shows, but the TIGAR is very obvious at 30 kDa.

Blocking

Blocking DetailsBlocking Buffer: TBS-T+5 % dry milk, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:4000, Incubation Dilution Buffer:TBS-T+5 % dry milk, Incubation Time:overnight, Incubation Temp:4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-rabbit, Secondary Ab Dilution Ratio: 1:4000

Details

Detection NotesECL, Exposure Time: 5 sec, Pos Ctrl: HepG2, Neg Ctrl: FOCUS, Specific Bands: 30 kDa, Non-Specific Bands: 42, 40, 26 kDa

Comments

CommentsWe have tested this TIGAR antibody is various human hepatocellular carcinoma cell lines. As the figure shows TIGAR is highly expressed in HepG2 cells, thus we use this cell line as positive control. Here we loaded about 50 ug, primary antibody was diluted to 1:4000 in TBS-T+5% dry milk (overnight incubation at 4 C). The antibody picks up 3 non-specific bands between as the picture shows, but the TIGAR is very obvious at 30 kDa.

Product General Protocols

View specific protocols for TIGAR/C12orf5 Antibody (NBP1-49534): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TIGAR/C12orf5 Antibody (NBP1-49534) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49534AF405
Alexa Fluor 488 Labeled NBP1-49534AF488
Alexa Fluor 647 Labeled NBP1-49534AF647
Alexa Fluor 700 Labeled NBP1-49534AF700
Biotin Labeled NBP1-49534B
DyLight 405 Labeled NBP1-49534V
DyLight 488 Labeled NBP1-49534G
DyLight 550 Labeled NBP1-49534R
DyLight 650 Labeled NBP1-49534C
FITC Labeled NBP1-49534F
HRP Labeled NBP1-49534H

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Recent Reviews

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1
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Anonymous
01/30/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol C12ORF5
Entrez
Uniprot