TIGAR/C12orf5 Antibody - BSA Free

Western Blot: TIGAR/C12orf5 Antibody [NBP1-49534] - Analysis of TIGAR in HeLa whole cell extracts.

Immunohistochemistry: TIGAR/C12orf5 Antibody [NBP1-49534] - Analysis of TIGAR in mouse muscle.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

TIGAR/C12orf5 Antibody - BSA Free Summary

• Immunogen: A genomic peptide made to an internal region of the human TIGAR protein (within residues 50-200). [Swiss-Prot Q9NQ88]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TIGAR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000
• Application Notes: This TIGAR antibody is useful for Immunohistochemistry-paraffin sections and Western blot. In Western blot bands are seen ~30 kDa, representing TIGAR, and ~47 kDa. At this time we cannot explain the band at 47 kDa. This antibody does not appear to work in ICC.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for TIGAR/C12orf5 Antibody - BSA Free

• C12orf5
• chromosome 12 open reading frame 5
• EC 3.1.3.46
• probable fructose-2,6-bisphosphatase TIGAR
• TIGAR
• TIGARTP53-induced glycolysis and apoptosis regulator
• transactivated by NS3TP2 protein

Background

TIGAR (TP53-induced glycolysis and apoptosis regulator), a member of phosphoglycerate mutase family, is a target gene of p53 and it regulates glucose metabolism in cancer cells by acting as a fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate (Fru-2,6-P2 ) and Fru-1,6-P2 leading to decreased Fru-2,6-P2 levels with subsequent reduction in PFK1 (phosphofructosekinase-1) activity as well as negative regulation of glycolysis. TIGAR also reduces cellular ROS through its translocation to the mitochondria where it interacts with HK2 leading to enhanced HK2 activity, regulation of mitochondrial membrane potential, and decreased mitochondrial ROS which ultimately protects the cells from DNA damage-induced apoptosis. Moreover, TIGAR mediated negative regulation of glycolysis leads to redirection of glycolytic metabolic intermediates towards pentose phosphate pathway's oxidative branch that follows increased cellular NADPH production, ROS scavenging by GSH and thus a lower sensitivity of cells to oxidative stress-associated apoptosis, including that induced by p53. Because of TIGAR's anti-apoptotic/protective role, it has been proposed to implicate in cell-cycle arrest and DNA repair effector response in cells that suffer from repairable dose of genotoxic/cellular insult.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for TIGAR/C12orf5 Antibody (NBP1-49534) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/30/2012

Summary

• Application: Western Blot
• Sample Tested: HepG2 total lysate, Sample Amount: ~ 50 ug
• Species: Human

Bioinformatics

• Gene Symbol: TIGAR
• Entrez:
  • 57103(Human)
  • 319801(Mouse)
• Uniprot:
  • Q9NQ88(Human)
  • Q8BZA9(Mouse)