Hexokinase 2 Antibody (OTI4C5)

Images

 
Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hexokinase 2I monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hexokinase 2.
Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells transfected with either pCMV6-ENTRY Hexokinase 2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Hexokinase 2 mouse ...read more
Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hexokinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of HepG2 cells using anti-Hexokinase 2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Ca, Mu, Rt, Po, EqSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI4C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Hexokinase 2 Antibody (OTI4C5) Summary

Immunogen
Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.
Specificity
Human Hexokinase-2 protein is 74% identical with Hexokinase-1 and our Hexokinase 2 antibody (clone 4C5) is not expected to crossreact with Hexokinase-1.
Predicted Species
Mouse (94%), Rat (94%), Porcine (96%), Equine (94%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HK2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
Theoretical MW
102.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-02272 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Hexokinase 2 Antibody (OTI4C5)

  • DKFZp686M1669
  • EC 2.7.1
  • Hexokinase 2
  • Hexokinase type II
  • HK2
  • HKII
  • HXK2
  • Muscle form hexokinase
  • muscle

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways.This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hexokinase 2 Antibody (NBP2-02272)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Hexokinase 2 Antibody (NBP2-02272) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70891AF350
Alexa Fluor 405 NBP2-70891AF405
Alexa Fluor 488 NBP2-70891AF488
Alexa Fluor 532 NBP2-70891AF532
Alexa Fluor 594 NBP2-70891AF594
Alexa Fluor 647 NBP2-70891AF647
Alexa Fluor 700 NBP2-70891AF700
Alexa Fluor 750 NBP2-70891AF750
Allophycocyanin NBP2-70891APC
Biotin NBP2-70891B
DyLight 350 NBP2-70891UV
DyLight 405 NBP2-70891V
DyLight 488 NBP2-70891G
DyLight 550 NBP2-70891R
DyLight 594 NBP2-70891DL594
DyLight 650 NBP2-70891C
DyLight 680 NBP2-70891FR
DyLight 755 NBP2-70891IR
FITC NBP2-70891F
HRP NBP2-70891H
Janelia Fluor 549 NBP2-70891JF549
Janelia Fluor 646 NBP2-70891JF646
PE NBP2-70891PE
PerCP NBP2-70891PCP

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Bioinformatics

Gene Symbol HK2
Entrez