XPE/DDB2 Antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPE/DDB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3297) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPE/DDB2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

XPE/DDB2 Antibody Summary

• Immunogen: E. coli-derived recombinant human XPE/DDB2
  Met1-Lys427
  Accession # Q92466
• Specificity: Detects human XPE/DDB2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DDB2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XPE/DDB2 Antibody

• damage-specific DNA binding protein 2 (48kD)
• damage-specific DNA binding protein 2, 48kDa
• Damage-specific DNA-binding protein 2
• DDB p48 subunit
• DDB2
• DDBB
• DNA damage-binding protein 2
• FLJ34321
• UV-damaged DNA-binding protein 2
• UV-DDB 2
• UV-DDB2
• xeroderma pigmentosum group E protein
• XP5
• XPE/DDB2

Background

Xeroderma Pigmentosum complementation group E (XPE) patients are defective in a heterodimer of damage-specific DNA-binding activity (DDB1/DDB2). DDB2 is a 48 kDa protein upregulated in cells exposed to ultraviolet light. XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XPE/DDB2 Antibody (AF3297)(4)

Publications using AF3297

• C Blessing, K Apelt, D van den He, C Gonzalez-L, MB Rother, M van der Wo, R González-P, A Yifrach, A Parnas, RG Shah, TT Kuo, DEC Boer, J Cai, A Kragten, HS Kim, OD Schärer, ACO Vertegaal, GM Shah, S Adar, H Lans, H van Attiku, AG Ladurner, MS Luijsterbu XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair Nature Communications, 2022-08-13;13(1):4762. 2022-08-13 [PMID: 35963869] (Western Blot, Human)
• W Sakai, M Yuasa-Suna, M Kusakabe, A Kishimoto, T Matsui, Y Kaneko, JI Akagi, N Huyghe, M Ikura, T Ikura, F Hanaoka, M Yokoi, K Sugasawa Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair Sci Rep, 2020-11-12;10(1):19704. 2020-11-12 [PMID: 33184426] (ICC, Human)
• TC Lai, MC Hu Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism Sci Rep, 2019-03-28;9(1):5304. 2019-03-28 [PMID: 30923324] (Western Blot, Human)
• Q Zhu, S Wei, N Sharma, G Wani, J He, AA Wani Human CRL4DDB2 ubiquitin ligase preferentially regulates post-repair chromatin restoration of H3K56Ac through recruitment of histone chaperon CAF-1 Oncotarget, 2017-10-17;8(61):104525-104542. 2017-10-17 [PMID: 29262658] (Western Blot, Human)

Bioinformatics

• Gene Symbol: DDB2
• Uniprot:
  • Q92466()