Immunocytochemistry/ Immunofluorescence: BNIP3 Antibody [NBP1-77683] - BNIP3 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: BNIP3 Antibody [NBP1-77683] - Analysis of BNIP3 in mouse kidney using DAB with hematoxylin counterstain.
Flow Cytometry: BNIP3 Antibody [NBP1-77683] - An intracellular stain was performed on PC12 cells with BNIP3 Antibody NBP1-77683F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: BNIP3 Antibody [NBP1-77683] - An intracellular stain was performed on PC12 cells with BNIP3 Antibody NBP1-77683AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
This BNIP3 antibody is useful for ICC/IF and Immunohistochemistry-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Western blot reported in scientific literature (PMID: 19574421).
Read Publications using NBP1-77683 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for BNIP3 Antibody
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
BCL2/adenovirus E1B 19kDa interacting protein 3
BCL2/adenovirus E1B 19kD-interacting protein 3
BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3) is a mitochondrial protein critical for apoptotic events in response to adenovirus infection. It has been implicated in causing a necrotic-like cell death pattern in cells, induced by CD47. This effect has been seen in many cancer cells, and its mis-regulation causes tumor cells to be impervious to normal autophagic events. The tumor suppressor p53 represses the expression of BNIP3, often as a consequence of hypoxic micro-environments as seen in tumors. When BNIP3 is correctly expressed and activated, it translocates to the mitochondria and instigates apoptosis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.