BNIP3 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: BNIP3 Antibody [NBP1-77683] - BNIP3 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: BNIP3 Antibody [NBP1-77683] - Analysis of BNIP3 in mouse kidney using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.17 mg/ml

BNIP3 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human BNIP3 protein (within residues 50-150). [Swiss-Prot Q12983]
Localization
Mitochondrion membrane. Note: Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BNIP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This BNIP3 antibody is useful for ICC/IF and Immunohistochemistry-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Publications
Read Publication using
NBP1-77683 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.17 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BNIP3 Antibody

  • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
  • BCL2/adenovirus E1B 19kDa interacting protein 3
  • BCL2/adenovirus E1B 19kD-interacting protein 3
  • BNIP3
  • Nip3

Background

BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3) is a mitochondrial protein critical for apoptotic events in response to adenovirus infection. It has been implicated in causing a necrotic-like cell death pattern in cells, induced by CD47. This effect has been seen in many cancer cells, and its mis-regulation causes tumor cells to be impervious to normal autophagic events. The tumor suppressor p53 represses the expression of BNIP3, often as a consequence of hypoxic micro-environments as seen in tumors. When BNIP3 is correctly expressed and activated, it translocates to the mitochondria and instigates apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BNIP3 Antibody (NBP1-77683)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(1)
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Mouse
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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for BNIP3 Antibody (NBP1-77683) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77683AF405
Alexa Fluor 488 Labeled NBP1-77683AF488
Alexa Fluor 647 Labeled NBP1-77683AF647
Alexa Fluor 700 Labeled NBP1-77683AF700
Biotin Labeled NBP1-77683B
DyLight 405 Labeled NBP1-77683V
DyLight 488 Labeled NBP1-77683G
DyLight 550 Labeled NBP1-77683R
DyLight 650 Labeled NBP1-77683C
FITC Labeled NBP1-77683F

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Blogs on BNIP3.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

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Bioinformatics

Gene Symbol BNIP3
Entrez
Uniprot