TIF1 alpha Antibody - BSA Free

Western Blot: TIF1 alpha Antibody [NB100-2597] - Detection of Human TIF1 alpha/TRIM24 on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-2597. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2596.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TIF1 alpha Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1,000 and the C-terminus (residue 1050) of human Transcriptional Intermediary Factor 1 Alpha (NP_056989.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM24
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in Immunohistochemistry-paraffin and Immunohistochemistry-Frozen reported in scientific literature (PMID: 23006314). Use in ICC/IF was reported in scientific literature (PMID: 23006314).
• Theoretical MW: 117 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TIF1 alpha Antibody - BSA Free

• E3 ubiquitin-protein ligase TRIM24
• EC 6.3.2
• EC 6.3.2.-
• hTIF1
• PTC6
• RING finger protein 82
• RNF82
• RNF82Tif1a
• TIF1 alpha
• TIF1
• TIF1A
• TIF1-alpha
• TIF1ATIF1ALPHA
• TIF1TF1A
• transcription intermediary factor 1-alpha
• transcriptional intermediary factor 1
• TRIM24
• tripartite motif containing 24
• tripartite motif-containing 24
• Tripartite motif-containing protein 24

Background

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIF1 alpha Antibody (NB100-2597)(4)

Publications using NB100-2597

• Gu Y, Zhang J, Ma X et al. Stabilization of the c-Myc Protein by CAMKIIgamma Promotes T Cell Lymphoma Cancer Cell 2017-07-10 [PMID: 28697340] (Human)
• Guo H, Liu W, Ju Z et al. An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays. Proteome Sci. 2012-09-24 [PMID: 23006314] (IHC-Fr, IHC-P, Human)
• Jain AK, Allton K, Iacovino M, Mahen E, Milczarek RJ, Zwaka TP, Kyba M, Barton MC. p53 Regulates Cell Cycle and MicroRNAs to Promote Differentiation of Human Embryonic Stem Cells. PLoS Biol;10(2):e1001268. 2012-02-01 [PMID: 22389628]
• Levin VA, Panchabhai SC, Shen L et al. Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines. J Proteome Res 2010-01-01 [PMID: 19894763] (Human)

Bioinformatics

• Gene Symbol: TRIM24
• Entrez:
  • 8805(Human)
  • 21848(Mouse)
• Uniprot:
  • O15164(Human)