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TRIM17 Antibody (2E11)

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Immunocytochemistry/Immunofluorescence: TRIM17 Antibody (2E11) [H00051127-M01] - Analysis of monoclonal antibody to TRIM17 on HeLa cell . Antibody concentration 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: TRIM17 Antibody (2E11) [H00051127-M01] - Analysis of monoclonal antibody to TRIM17 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: TRIM17 Antibody (2E11) [H00051127-M01] - Detection limit for recombinant GST tagged TRIM17 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, PLA, S-ELISA
Clone
2E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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TRIM17 Antibody (2E11) Summary

Immunogen
TRIM17 (NP_057186.1, 75 a.a. ~ 184 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LPNRLLTKVAEMAQQHPGLQKQDLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEAVQGYKLKLEEDMEYLREQITRTGNLQAREEQSLAEWQGKVKERRER
Specificity
TRIM17 - tripartite motif-containing 17 (2E11)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TRIM17
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Publications
Read Publication using H00051127-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRIM17 Antibody (2E11)

  • E3 ubiquitin-protein ligase TRIM17
  • EC 6.3.2.-
  • RBCCRNF16terf
  • RING finger protein 16
  • RING finger protein terf
  • TERF
  • Testis RING finger protein
  • tripartite motif containing 17
  • tripartite motif-containing 17
  • Tripartite motif-containing protein 17

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein is expressed almost exclusively in the testis, but its function is unknown. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of human peripheral blood mononuclear cells and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRIM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4708) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRIM at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Human CD3+ T cells from peripheral blood mononuclear cells were stained with Goat Anti-Human TRIM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4708, filled histo­gram) or isotype control antibody (Catalog #AB-108-C, open histo­gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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MID1 Antibody
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Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody . Blue: DAPI for nuclear staining.
Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.
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Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on formalin-fixed paraffin-embedded human testis. Antibody concentration 6 ug/ml.
Immunoprecipitation: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of TRIM24 transfected lysate using anti-TRIM24 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TRIM24 MaxPab rabbit polyclonal antibody.
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Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - FFPE serial sections of asynchronous HeLa cells treated with EPE (left) anduntreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). Detection: DAB
Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350] - NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with EPE (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.
(1 Review)
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Species: Hu, Mu, Rt
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Western Blot: MPG Antibody [NBP1-82787] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunohistochemistry-Paraffin: MPG Antibody [NBP1-82787] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Immunocytochemistry/Immunofluorescence: MPG Antibody [NBP1-82787] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.
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(2 Publications)
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Western Blot: RBCK1 Antibody [NBP1-88301] - Analysis in human cell lines SK-MEL-30 and HeLa using anti-RBCK1 antibody. Corresponding RBCK1 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
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Western Blot: PML Protein Antibody [NB100-59787] - PML-RARa is a HIF-I+-transcriptional co-activator. HEK-293 cells transfected with stable mutants of HIF-1I+-or HIF-2I+-and increasing concentrations of PML-RARI+-. Image collected and cropped by Citeab from the following publication (HIF factors cooperate with PML-RAR +/- to promote acute promyelocytic leukemia progression and relapse. EMBO Mol Med (2014)) licensed under a CC-BY licence.
Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
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Immunohistochemistry-Paraffin: ZNF645 Antibody [NBP2-21037] - Immunohistochemical analysis of paraffin-embedded GASTRIC CA, using antibody at 1:500 dilution.
Species: Hu
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Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a section of normal prostate from human using 5 ug/ml concentration of MUL1 antibody (clone 5H6.2D5). Strong cytoplasmic staining was observed in the epithelial columnar and basal cells of prostate glands.
Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a section of normal cardiac stomach from human using 5 ug/ml concentration of MUL1 antibody (clone 5H6.2D5). The cardiac glands showed distinct cytoplasmic staining, wherein, the parietal cells as well as the chief cells were found positive for MUL1.
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Applications: IHC, IHC-P, WB
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Immunohistochemistry-Paraffin: TRIM50 Antibody [NBP1-81988] - Staining of human bone marrow shows strong cytoplasmic positivity in a subset of bone marrow poietic cells.
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Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western Blot: ILT6/CD85e/LILRA3 Antibody [NBP1-98549] - Antibody Dilution: 1.0ug/ml Sample Tissue: Human Kidney.
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Immunohistochemistry: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: TRIM27 Antibody (4D4) [NBP2-46214] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample(30 ug of whole cell lysate) A:293T 10% SDS PAGE, antibody diluted at 1:1000.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
(1 Review)
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Immunohistochemistry-Paraffin: TRIM13 Antibody [NBP1-85016] - Analysis in human testis and pancreas tissues. Corresponding TRIM13 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: TRIM13 Antibody [NBP1-85016] - Staining of human duodenum shows no positivity in glandular cells as expected.
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Pyruvate
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Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NBP1-49536) at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - Characterization of MPC1-/- in the RM-1 murine prostate cancer cells. Western blotting results of subcellular proteins from WT and MPC1-/- cells and corresponding densitometry histograms of ALT1 and Pyruvate Carboxylase. Cyto means cytoplasmic protein. Mito stands for mitochondrial protein. Protein alpha-Tublin was used as a loading control. ***P<0.001. All experiments were performed at least three times with consistent and repeatable results. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199), licensed under a CC-BY licence.
Knockdown Validated: Pyruvate Carboxylase Antibody [NBP1-49536] - HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. Divergent effects of human cytomegalovirus and herpes simplex virus-1 on cellular metabolism. PLoS Pathog (2011)
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Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536] - Staining of human urinary bladder shows high expression.
Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536] - Staining of human cell line hTCEpi shows localization to intermediate filaments. Antibody staining is shown in green.
(1 Review)
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RNF98 Antibody
(2D11)
H00055521-M01
Immunocytochemistry/Immunofluorescence: RNF98 Antibody (2D11) [H00055521-M01] - Analysis of monoclonal antibody to TRIM36 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: RNF98 Antibody (2D11) [H00055521-M01] - Analysis of monoclonal antibody to TRIM36 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Western Blot: RNF98 Antibody (2D11) [H00055521-M01] - TRIM36 monoclonal antibody (M01), clone 2D11 Analysis of TRIM36 expression in IMR-32.
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB

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Bioinformatics

Gene Symbol TRIM17
Entrez
Uniprot