KAP1 [p Ser824] Antibody


Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody ...read more
Immunocytochemistry/ Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350] - NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with EPE (left) or untreated (right). Antibody: ...read more
Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). ...read more
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (15 ug) from NIH 3T3 cellstreated with 100 uM etoposide (+) or mock treated (-).Antibodies: Affinity purified rabbit anti-Phospho ...read more
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (50 ug) from HEK293Tcells treated with 100 uM etoposide (+) or mock treated(-). Antibodies: Affinity purified rabbit anti-Phospho ...read more
Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - FFPE serial sections of asynchronous HeLa cells treated with EPE (left) anduntreated HeLa cells (right). Antibody: Affinity purified rabbit ...read more
Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
0.2 mg/ml

Order Details

KAP1 [p Ser824] Antibody Summary

This KAP1 [p Ser824] Antibody was developed against mmunogen a synthetic phosphorylated peptide, which represents a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).
p Ser824
Immunogen affinity purified
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  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618) Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. KAP1 [p Ser824] antibody validated for WB from a verified customer review.
Read Publications using
NB100-2350 in the following applications:

  • 1 publication
  • WB
    10 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24248351). Rat reactivity reported in scientific literature (PMID: 27895165).

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
TBS, 0.1% BSA
0.09% Sodium Azide
0.2 mg/ml
Immunogen affinity purified


This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB500-158, NB100-41429

Alternate Names for KAP1 [p Ser824] Antibody

  • E3 SUMO-protein ligase TRIM28
  • EC 6.3.2.-
  • FLJ29029
  • KAP1
  • KAP-1
  • KAP1KRAB-associated protein 1
  • KRIP-1
  • Nuclear corepressor KAP-1
  • RNF96
  • RNF96KRAB-interacting protein 1
  • TF1B
  • TIF1B
  • TIF1-beta
  • TIF1BRING finger protein 96
  • transcription intermediary factor 1-beta
  • transcriptional intermediary factor 1-beta
  • TRIM28
  • tripartite motif containing 28
  • tripartite motif-containing 28
  • Tripartite motif-containing protein 28


The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
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Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Bt, Bv, Ca, Fi, Gt, Hu, Mu, Pm, Rt
Applications: ChIP, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, KD, KO, WB
Species: Ce, Dr, Hu, In, Ma, Mu, Po, Rt, Ye
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Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, IP, KD, PLA, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Rt
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Species: Hu
Applications: CyTOF-ready, ICFlow
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ELISA, ICC/IF, PLA, S-ELISA, WB
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP

Publications for KAP1 Antibody (NB100-2350)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ChIP, WB.

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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB100-2350 Applications Species
Nayler S, Gatei M, Kozlov S et al. Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells: Induced Pluripotent Stem Cells from Ataxia-Telangiectasia Recapitulate the Cellular Phenotype Stem Cells Trans Med 2012 June (WB, Human) WB Human
Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications Jan 14 2022 [PMID: 35031618] (ChIP, WB) ChIP, WB
Iida K, Abdelhamid Ahmed AH, Nagatsuma AK et al. Identification and Therapeutic Targeting of GPR20, Selectively Expressed in Gastrointestinal Stromal Tumors, with DS-6157a, a First-in-Class Antibody-Drug Conjugate Cancer discovery Feb 12 2021 [PMID: 33579785]
Martinikova AS, Burocziova M, Stoyanov M, Macurek L Truncated PPM1D Prevents Apoptosis in the Murine Thymus and Promotes Ionizing Radiation-Induced Lymphoma Cells Sep 10 2020 [PMID: 32927737] (WB, Mouse) WB Mouse
Quek H, Luff J, Cheung K et al. Rats with a missense mutation in Atm display neuroinflammation and neurodegeneration subsequent to accumulation of cytosolic DNA following unrepaired DNA damage. J. Leukoc. Biol. Nov 28 2016 [PMID: 27895165] (Rat) Rat
Hartlerode AJ, Morgan MJ, Wu Y et al. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat. Struct. Mol. Biol. 2015 Aug 17 [PMID: 26280532] (WB, Mouse) WB Mouse
Kijas AW, Lim YC, Bolderson E et al. ATM-dependent phosphorylation of MRE11 controls extent of resection during homology directed repair by signalling through Exonuclease 1. Nucleic Acids Res. 2015 Aug 03 [PMID: 26240375] (WB, Human) WB Human
Puccini J, Shalini S, Voss AK et al. Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice. Proc Natl Acad Sci U S A 2013 Dec 3 [PMID: 24248351] (WB, Mouse) WB Mouse
Bhatia N, Xiao TZ, Rosenthal KA et al. MAGE-C2 Promotes Growth and Tumorigenicity of Melanoma Cells, Phosphorylation of KAP1, and DNA Damage Repair J Invest Dermatol 2012 Oct 25 [PMID: 23096706] (WB, Human) WB Human
Yamamoto K, Wang Y, Jiang W et al. Kinase-dead ATM protein causes genomic instability and early embryonic lethality in mice. J Cell Biol 2012 Aug [PMID: 22869596]
Show All 33 Publications.

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Product General Protocols

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WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Gene Symbol TRIM28