Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody ...read more
Immunocytochemistry/ Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350] - NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with EPE (left) or untreated (right). Antibody: ...read more
Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). ...read more
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (15 ug) from NIH 3T3 cellstreated with 100 uM etoposide (+) or mock treated (-).Antibodies: Affinity purified rabbit anti-Phospho ...read more
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (50 ug) from HEK293Tcells treated with 100 uM etoposide (+) or mock treated(-). Antibodies: Affinity purified rabbit anti-Phospho ...read more
Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - FFPE serial sections of asynchronous HeLa cells treated with EPE (left) anduntreated HeLa cells (right). Antibody: Affinity purified rabbit ...read more
Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit ...read more
This KAP1 [p Ser824] Antibody was developed against mmunogen a synthetic phosphorylated peptide, which represents a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618) Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. KAP1 [p Ser824] antibody validated for WB from a verified customer review.
Read Publications using NB100-2350 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 24248351). Rat reactivity reported in scientific literature (PMID: 27895165).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
TBS, 0.1% BSA
0.09% Sodium Azide
Immunogen affinity purified
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB500-158, NB100-41429
Alternate Names for KAP1 [p Ser824] Antibody
E3 SUMO-protein ligase TRIM28
KAP1KRAB-associated protein 1
Nuclear corepressor KAP-1
RNF96KRAB-interacting protein 1
TIF1BRING finger protein 96
transcription intermediary factor 1-beta
transcriptional intermediary factor 1-beta
tripartite motif containing 28
tripartite motif-containing 28
Tripartite motif-containing protein 28
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Nayler S, Gatei M, Kozlov S et al. Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells: Induced Pluripotent Stem Cells from Ataxia-Telangiectasia Recapitulate the Cellular Phenotype Stem Cells Trans Med 2012 June (WB, Human)
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