Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody ...read more
Immunocytochemistry/ Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350] - NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with EPE (left) or untreated (right). Antibody: ...read more
Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). ...read more
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (15 ug) from NIH 3T3 cellstreated with 100 uM etoposide (+) or mock treated (-).Antibodies: Affinity purified rabbit anti-Phospho ...read more
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (50 ug) from HEK293Tcells treated with 100 uM etoposide (+) or mock treated(-). Antibodies: Affinity purified rabbit anti-Phospho ...read more
Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - FFPE serial sections of asynchronous HeLa cells treated with EPE (left) anduntreated HeLa cells (right). Antibody: Affinity purified rabbit ...read more
Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit ...read more
This KAP1 [p Ser824] Antibody was developed against mmunogen a synthetic phosphorylated peptide, which represents a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).
Modification
p Ser824
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM28
Purity
Immunogen affinity purified
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Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. KAP1 [p Ser824] antibody validated for WB from a verified customer review.
Mouse reactivity reported in scientific literature (PMID: 24248351). Rat reactivity reported in scientific literature (PMID: 27895165).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
TBS, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified
Notes
This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB500-158, NB100-41429
Alternate Names for KAP1 [p Ser824] Antibody
E3 SUMO-protein ligase TRIM28
EC 6.3.2.-
FLJ29029
KAP1
KAP-1
KAP1KRAB-associated protein 1
KRIP-1
Nuclear corepressor KAP-1
RNF96
RNF96KRAB-interacting protein 1
TF1B
TIF1B
TIF1-beta
TIF1BRING finger protein 96
transcription intermediary factor 1-beta
transcriptional intermediary factor 1-beta
TRIM28
tripartite motif containing 28
tripartite motif-containing 28
Tripartite motif-containing protein 28
Background
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Nayler S, Gatei M, Kozlov S et al. Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells: Induced Pluripotent Stem Cells from Ataxia-Telangiectasia Recapitulate the Cellular Phenotype Stem Cells Trans Med 2012 June (WB, Human)
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Diseases for KAP1 Antibody (NB100-2350)
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.