KAP1 [p Ser824] Antibody

Images

 
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Detection Human Phospho KAP-1 (S824) on HeLa whole cell lysate. KAP-1 was immunoprecipitated using another rabbit anti-KAP-1 antibody BL553.
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from NIH3T3 cells.
Proximity Ligation Assay: KAP1 [p Ser824] Antibody [NB100-2350] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human phospho-KAP1 [S824] (NB100-2350). Image merged ...read more
Proximity Ligation Assay: KAP1 [p Ser824] Antibody [NB100-2350] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human phospho-KAP1 [S824] (NB100-2350). Image ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

KAP1 [p Ser824] Antibody Summary

Immunogen
Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).
Modification
p Ser824
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:10000
Publications
Read Publications using
NB100-2350 in the following applications:

  • WB
    8 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24248351) Expected to cross react based on 100% sequence identity: Orangutan, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque and Northern white-cheeked gibbon.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB500-158, NB100-41429

Alternate Names for KAP1 [p Ser824] Antibody

  • E3 SUMO-protein ligase TRIM28
  • EC 6.3.2.-
  • FLJ29029
  • KAP1
  • KAP-1
  • KAP1KRAB-associated protein 1
  • KRIP-1
  • Nuclear corepressor KAP-1
  • RNF96
  • RNF96KRAB-interacting protein 1
  • TF1B
  • TIF1B
  • TIF1-beta
  • TIF1BRING finger protein 96
  • transcription intermediary factor 1-beta
  • transcriptional intermediary factor 1-beta
  • TRIM28
  • tripartite motif containing 28
  • tripartite motif-containing 28
  • Tripartite motif-containing protein 28

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
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Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
NB500-171
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NB100-2350
Species: Hu, Mu
Applications: WB, ICC/IF, IP, PLA

Publications for KAP1 Antibody (NB100-2350)(30)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.


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WB
(8)
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Human
(6)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB100-2350 Applications Species
Quek H, Luff J, Cheung K et al. Rats with a missense mutation in Atm display neuroinflammation and neurodegeneration subsequent to accumulation of cytosolic DNA following unrepaired DNA damage. J. Leukoc. Biol. Nov 28 2016 [PMID: 27895165] (Rat) Rat
Hartlerode AJ, Morgan MJ, Wu Y et al. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat. Struct. Mol. Biol. 2015 Aug 17 [PMID: 26280532] (WB, Mouse) WB Mouse
Kijas AW, Lim YC, Bolderson E et al. ATM-dependent phosphorylation of MRE11 controls extent of resection during homology directed repair by signalling through Exonuclease 1. Nucleic Acids Res. 2015 Aug 03 [PMID: 26240375] (WB, Human) WB Human
Puccini J, Shalini S, Voss AK et al. Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice. Proc Natl Acad Sci U S A 2013 Dec 3 [PMID: 24248351] (WB, Mouse) WB Mouse
Bhatia N, Xiao TZ, Rosenthal KA et al. MAGE-C2 Promotes Growth and Tumorigenicity of Melanoma Cells, Phosphorylation of KAP1, and DNA Damage Repair J Invest Dermatol 2012 Oct 25 [PMID: 23096706] (WB, Human) WB Human
Yamamoto K, Wang Y, Jiang W et al. Kinase-dead ATM protein causes genomic instability and early embryonic lethality in mice. J Cell Biol 2012 Aug [PMID: 22869596]
Bolderson E, Savage KI, Mahen R et al. Kruppel-associated Box (KRAB)-associated co-repressor (KAP-1) Ser-473 phosphorylation regulates heterochromatin protein 1beta (HP1-beta) mobilization and DNA repair in heterochromatin. J Biol Chem 2012 Aug [PMID: 22715096]
Pires IM, Olcina MM, Anbalagan S et al. Targeting radiation-resistant hypoxic tumour cells through ATR inhibition. Br J Cancer 2012 Jul [PMID: 22713662]
Temviriyanukul P, van Hees-Stuivenberg S, Delbos F et al. Temporally distinct translesion synthesis pathways for ultraviolet light-induced photoproducts in the mammalian genome. DNA Repair (Amst) 2012 Jun [PMID: 22521143]
Lee DH, Goodarzi AA, Adelmant GO et al. Phosphoproteomic analysis reveals that PP4 dephosphorylates KAP-1 impacting the DNA damage response. EMBO J 2012 Apr [PMID: 22491012]
Show All 30 Publications.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRIM28
Entrez
Uniprot