KAP1 [p Ser824] Antibody


Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Detection Human Phospho KAP-1 (S824) on HeLa whole cell lysate. KAP-1 was immunoprecipitated using another rabbit anti-KAP-1 antibody BL553.
Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from NIH3T3 cells.
Proximity Ligation Assay: KAP1 [p Ser824] Antibody [NB100-2350] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human phospho-KAP1 [S824] (NB100-2350). Image merged ...read more
Proximity Ligation Assay: KAP1 [p Ser824] Antibody [NB100-2350] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human phospho-KAP1 [S824] (NB100-2350). Image ...read more

Product Details

Reactivity Hu, Mu, Primate, Rhesus MacaqueSpecies Glossary
Applications WB, ICC/IF, IP, PLA
0.2 mg/ml

Order Details

KAP1 [p Ser824] Antibody Summary

Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).
p Ser824
Predicted Species
Rhesus Macaque (100%), Primate (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
0.2 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:10000
Read Publications using
NB100-2350 in the following applications:

  • WB
    8 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24248351) Expected to cross react based on 100% sequence identity: Orangutan, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque and Northern white-cheeked gibbon.


This anitbody can be used as the primaryantibody in a PLA assay with the following as secondary antibodies: NB100-322, NB500-158, NB100-41429

Alternate Names for KAP1 [p Ser824] Antibody

  • E3 SUMO-protein ligase TRIM28
  • EC 6.3.2.-
  • FLJ29029
  • KAP-1
  • KAP1KRAB-associated protein 1
  • KRIP-1
  • Nuclear corepressor KAP-1
  • RNF96
  • RNF96KRAB-interacting protein 1
  • TF1B
  • TIF1B
  • TIF1-beta
  • TIF1BRING finger protein 96
  • transcription intermediary factor 1-beta
  • transcriptional intermediary factor 1-beta
  • TRIM28
  • tripartite motif containing 28
  • tripartite motif-containing 28
  • Tripartite motif-containing protein 28


The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KAP1 Antibody (NB100-2350)(29)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB100-2350 Applications Species
Hartlerode AJ, Morgan MJ, Wu Y et al. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat. Struct. Mol. Biol. 2015 Aug 17 [PMID:26280532] (WB, Mouse) WB Mouse
Kijas AW, Lim YC, Bolderson E et al. ATM-dependent phosphorylation of MRE11 controls extent of resection during homology directed repair by signalling through Exonuclease 1. Nucleic Acids Res. 2015 Aug 03 [PMID:26240375] (WB, Human) WB Human
Puccini J, Shalini S, Voss AK et al. Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice. Proc Natl Acad Sci U S A 2013 Dec 3 [PMID:24248351] (WB, Mouse) WB Mouse
Bhatia N, Xiao TZ, Rosenthal KA et al. MAGE-C2 Promotes Growth and Tumorigenicity of Melanoma Cells, Phosphorylation of KAP1, and DNA Damage Repair J Invest Dermatol 2012 Oct 25 [PMID:23096706] (WB, Human) WB Human
Yamamoto K, Wang Y, Jiang W et al. Kinase-dead ATM protein causes genomic instability and early embryonic lethality in mice. J Cell Biol 2012 Aug [PMID:22869596]
Bolderson E, Savage KI, Mahen R et al. Kruppel-associated Box (KRAB)-associated co-repressor (KAP-1) Ser-473 phosphorylation regulates heterochromatin protein 1beta (HP1-beta) mobilization and DNA repair in heterochromatin. J Biol Chem 2012 Aug [PMID:22715096]
Pires IM, Olcina MM, Anbalagan S et al. Targeting radiation-resistant hypoxic tumour cells through ATR inhibition. Br J Cancer 2012 Jul [PMID:22713662]
Temviriyanukul P, van Hees-Stuivenberg S, Delbos F et al. Temporally distinct translesion synthesis pathways for ultraviolet light-induced photoproducts in the mammalian genome. DNA Repair (Amst) 2012 Jun [PMID:22521143]
Lee DH, Goodarzi AA, Adelmant GO et al. Phosphoproteomic analysis reveals that PP4 dephosphorylates KAP-1 impacting the DNA damage response. EMBO J 2012 Apr [PMID:22491012]
Xiao TZ, Bhatia N, Urrutia R et al. MAGE I Transcription Factors Regulate KAP1 and KRAB Domain Zinc Finger Transcription Factor Mediated Gene Repression. PLoS ONE. 20116(8):e23747. [PMID:21876767]
Show All 29 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Contact Information

Product PDFs


Gene Symbol TRIM28

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