NCOA2 Antibody

Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative images taken from estradiol-treated wt mice. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY licence.

Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of prostate adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:100. Detection : DAB

Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative image taken from vehicle control wt mice Insets show the magnified image of an area within the small square box. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY licence.

Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Samples: FFPE sections of human breast adenocarcinoma (upper image) and prostate adenocarcinoma (lower image). Antibody: Affinity purified rabbit anti-SRC2 NB100-1756 used at a dilution of 1:100. Detection: DAB

Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of human breast adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:250. Detection : DAB

• Reactivity: Hu, Mu, Pm, RM
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.25 mg/ml

NCOA2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1400 and the C-terminus (residue 1464) of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).
• Predicted Species: Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCOA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
• Application Notes: Likely to work in frozen sections. Although not tested this antibody may be useful in ICC/IF. *The investigator should determine the optimal working dilution for a specific application. Use in Western blot reported in scientific literature (PMID: 26465008).
• Control:

  NCOA2 Overexpression Lysate

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Gorilla, White-tufted-ear Marmoset and Northern White-cheeked Gibbon. Mouse reactivity reported in scientific literature (PMID: 26465008).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.25 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCOA2 Antibody

• bHLHe75
• bHLHe75MGC138808
• Class E basic helix-loop-helix protein 75
• EC 2.3.1.48
• GRIP1glucocorticoid receptor-interacting protein-1
• HTIF2
• KAT13C
• NCOA2
• NCoA-2SRC2
• nuclear receptor coactivator 2
• TIF2
• TIF2hTIF2
• Transcriptional intermediary factor 2

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-2 (SRC2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCOA2 Antibody (NB100-1756)(3)

Publications using NB100-1756

• Acharya KD, Finkelstein SD, Bless EP et al. Estradiol Preferentially Induces Progestin Receptor-A (PR-A) Over PR-B in Cells Expressing Nuclear Receptor Coactivators in the Female Mouse Hypothalamus(1,2,3). eNeuro 2015 Jul-Aug [PMID: 26465008] (WB, Mouse)
• Niessen NA, Balthazart J, Ball GF, Charlier TD. Steroid receptor coactivator 2 modulates steroid-dependent male sexual behavior and neuroplasticity in Japanese quail (Coturnix japonica). J Neurochem 2011 Nov [PMID: 21854393]
• Tognoni CM, Chadwick JG Jr, Ackeifi CA, Tetel MJ. Nuclear receptor coactivators are coexpressed with steroid receptors and regulated by estradiol in mouse brain. Neuroendocrinology;94(1):49-57. 2011 [PMID: 21311177] (IHC, Mouse)

Review for NCOA2 Antibody (NB100-1756) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse and Rat.

reviewed by: Anonymous ICC/IF Mouse and Rat 10/06/2016

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Sample Tested: Brain (hypothalamus) tissue
• Species: Mouse and Rat

Bioinformatics

• Gene Symbol: NCOA2
• Entrez:
  • 10499(Human)
• Uniprot:
  • Q15596(Human)