NCOA2 Antibody

Images

 
Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of human breast adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:250. Detection : DAB
Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Samples: FFPE sections of human breast adenocarcinoma (upper image) and prostate adenocarcinoma (lower image). Antibody: Affinity purified rabbit anti-SRC2 ...read more
Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of prostate adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:100. Detection : DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NCOA2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1400 and the C-terminus (residue 1464) of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCOA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
Likely to work in frozen sections. Although not tested this antibody may be useful in ICC/IF. *The investigator should determine the optimal working dilution for a specific application.
Positive Control
NCOA2 Lysate (NBL1-13516)
Reviewed Applications
Read 1 Review rated 4
using
NB100-1756 in the following applications:

Publications
Read Publications using
NB100-1756 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset and Northern white-cheeked gibbon. Mouse reactivity reported in scientific literature (PMID: 26465008).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NCOA2 Antibody

  • bHLHe75
  • bHLHe75MGC138808
  • Class E basic helix-loop-helix protein 75
  • EC 2.3.1.48
  • GRIP1glucocorticoid receptor-interacting protein-1
  • HTIF2
  • KAT13C
  • NCOA2
  • NCoA-2SRC2
  • nuclear receptor coactivator 2
  • TIF2
  • TIF2hTIF2
  • Transcriptional intermediary factor 2

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-2 (SRC2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NCOA2 Antibody (NB100-1756)(3)

Review for NCOA2 Antibody (NB100-1756) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse and Rat.

Reviews using NB100-1756:
Filter by Applications
ICC/IF
(1)
All Applications
Filter by Species
Mouse and Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence NCOA2 NB100-1756
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reviewed by:
Anonymous
ICC/IF Mouse and Rat 10/06/2016
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedBrain (hypothalamus) tissue
SpeciesMouse and Rat

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for NCOA2 Antibody (NB100-1756) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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1
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Anonymous
10/06/2016
Application: ICC/IF
Species: Mouse and Rat

Bioinformatics

Gene Symbol NCOA2
Entrez
Uniprot