Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed ...read more
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PML used at a dilution of 1:500
Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) ...read more
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolin II PLA in Hela cells. goat anti-human HP1-beta (NB100-79774) and rabbit anti-human PML (NB100-59787). Image merged from DAPI ...read more
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) ...read more
Immunohistochemistry 1:200 to 1:1,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in Immunoblotting reported in scientific literature (PMID 28239645). Knockdown and Flow Cytometry validation (PMID: 28402725).
Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee, Primates, White-tufted-ear marmoset, Pygmy chimpanzee and Bornean orangutan. Mouse reactivity reported in scientific literature (PMID; 21343252)
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for PML Protein Antibody
Promyelocytic leukemia protein
RING finger protein 71
RNF71probable transcription factor PML
TRIM19promyelocytic leukemia, inducer of
tripartite motif protein TRIM19
Tripartite motif-containing protein 19
PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.