PML Protein Antibody - BSA Free

Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed using HRP-labeled secondary with DAB reagent followed by hematoxylin counterstaining. This antibody generated an expected strong nuclear staining in most of the cells in the glandular as well as the connective tissue. The cells showed some cytoplasmic signal also which was much weaker than the nuclear staining.

Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.

Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Western Blot: PML Protein Antibody [NB100-59787] - PML-RARa is a HIF-I+-transcriptional co-activator. HEK-293 cells transfected with stable mutants of HIF-1I+-or HIF-2I+-and increasing concentrations of PML-RARI+-. Image collected and cropped by Citeab from the following publication (HIF factors cooperate with PML-RAR +/- to promote acute promyelocytic leukemia progression and relapse. EMBO Mol Med (2014)) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with PML Protein Antibody (NB100-59787) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with PML Protein Antibody (NB100-59787) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PML used at a dilution of 1:500

Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

• Reactivity: Hu, Mu, Pm, Pm
• Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, IP, PLA, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PML Protein Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 375 and 425 of promyelocytic leukemia using the numbering given in entry NP_150241.2
• Predicted Species: Primate (100%), Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PML
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Proximity Ligation Assay 1:200-1:5000
  • Western Blot 1:2000-1:10000
• Application Notes: Immunohistochemistry 1:200 to 1:1,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in Immunoblotting reported in scientific literature (PMID 28239645). Knockdown and Flow Cytometry validation (PMID: 28402725).

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee, Primates, White-tufted-ear marmoset, Pygmy chimpanzee and Bornean orangutan. Mouse reactivity reported in scientific literature (PMID; 21343252)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PML Protein Antibody - BSA Free

• MYLPP8675
• Promyelocytic leukemia protein
• promyelocytic leukemia
• RING finger protein 71
• RNF71probable transcription factor PML
• TRIM19promyelocytic leukemia, inducer of
• tripartite motif protein TRIM19
• Tripartite motif-containing protein 19

Background

PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PML Protein Antibody (NB100-59787)(34)

Publications using NB100-59787

• Yan HY, Wang HQ, Zhong M et al. PML Suppresses Influenza Virus Replication by Promoting FBXW7 Expression Virologica Sinica May 27 2021 [PMID: 34046815]
• Lee J, Stone J, Desai P et al. Arsenicals, the Integrated Stress Response, and Epstein-Barr Virus Lytic Gene Expression Viruses Apr 30 2021 [PMID: 33946406] (WB, Human)
• Marks D, Heinen N, Bachmann L et al. Amyloid precursor protein elevates fusion of promyelocytic leukemia nuclear bodies in human hippocampal areas with high plaque load Acta neuropathologica communications Apr 13 2021 [PMID: 33849647] (IHC, Human)
• Karle W, Becker S, Stenzel P et al. Promyelocytic leukemia protein (PML) promotes the phenotypic switch of smooth muscle cells in atherosclerotic plaques of human coronary arteries Clinical science (London, England : 1979) Mar 25 2021 [PMID: 33764440]
• Becker S, Steinemann G, Karle W et al. Stability of Smyd1 in endothelial cells is controlled by PML-dependent SUMOylation upon cytokine Stimulation Nature Nov 25 2020 [PMID: 33241844]
• Grigore F, Yang H, Hanson ND et al. BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases Neoplasia Jul 3 2020 [PMID: 32629178]
• Hofmann S, Mai J, Masser S et Al. ATO (Arsenic Trioxide) Effects on Promyelocytic Leukemia Nuclear Bodies Reveals Antiviral Intervention Capacity Adv. Sci. Feb 27 2020 [PMID: 32328411] (WB)
• Stubbe M, Mai J, Paulus C et Al. Viral DNA Binding Protein SUMOylation Promotes PML Nuclear Body Localization Next to Viral Replication Centers mBio Mar 17 2020 [PMID: 32184235] (ICC/IF, Human)
• Chen YT, Ou Yang WT, Juang HH et al. Proteomic characterization of arsenic and cadmium exposure in bladder cells Rapid Commun. Mass Spectrom. Sep 9 2019 [PMID: 31499585]
• Li K, Wang F et al. TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RAR alpha and Inhibition of p53-Mediated Senescence. Cancer Cell Aug 5 2017 [PMID: 28486108] (WB, Mouse)
• LAng A, Eriksson J, Schink KO et al. Visualization of PML nuclear import complexes reveals FG-repeat nucleoporins at cargo retrieval sites Nucleus. 2017 Apr 11 [PMID: 28402725] (KD, WB, Flow, Human)
• Yao K, Wu Y, Chen Q et al. The Arginine/Lysine-Rich Element within the DNA-Binding Domain Is Essential for Nuclear Localization and Function of the Intracellular Pathogen Resistance 1 PLoS ONE Sep 14 2016 [PMID: 27622275] (ICC/IF, Human)
• Neumann F, Czech-Sioli M et al. Replication of Merkel cell polyomavirus induces reorganization of promyelocytic leukemia nuclear bodies. J Gen Virol Jan 11 2016 [PMID: 27580912] (WB, Human)
• Komatsu T, Nagata K, Wodrich H. An adenovirus DNA replication factor, but not incoming genome complexes, targets PML nuclear bodies. J. Virol. 2015 Nov 25 [PMID: 26608315]
• Burck C, Mund A, Berscheminski J et al. KAP1 Is a Host Restriction Factor That Promotes Human Adenovirus E1B-55K SUMO Modification. J Virol 2015 Nov 04 [PMID: 26537675]
• Berscheminski J, Brun J, Speiseder T et al. Sp100A is a tumor suppressor that activates p53-dependent transcription and counteracts E1A/E1B-55K-mediated transformation. Oncogene 2015 Oct 19 [PMID: 26477309]
• Wimmer P, Berscheminski J, Blanchette P et al. PML isoforms IV and V contribute to adenovirus-mediated oncogenic transformation by functionally inhibiting the tumor-suppressor p53. Oncogene. 2015 Mar 16 [PMID: 25772236] (ICC/IF, Human)
• Chen HY, Hu JY, Chen TH et al. KLHL39 suppresses colon cancer metastasis by blocking KLHL20-mediated PML and DAPK ubiquitination Oncogene. 2015 Jan 26 [PMID: 25619834] (WB)
• Coltella N, Percio S, Valsecchi R et al. HIF factors cooperate with PML-RARalpha to promote acute promyelocytic leukemia progression and relapse. EMBO Mol Med 2014 Apr 07 [PMID: 24711541] (WB, IP, Human)
• Palibrk V, Lang E, Lang A et al. Promyelocytic leukemia bodies tether to early endosomes during mitosis. Cell Cycle 2014 [PMID: 24675887] (Human)
• Berscheminski J, Wimmer P, Brun J et al. Sp100 isoform-specific regulation of human Adenovirus type 5 (Ad5) gene expression. J. Virol. 3/12/2014 [PMID: 24623443] (WB, ICC/IF, Human)
• Schreiner S, Burck C, Glass M et al. Control of human adenovirus type 5 gene expression by cellular Daxx/ATRX chromatin-associated complexes. Nucleic Acids Res 2013 Feb 8 [PMID: 23396441] (WB, Human)
• Berscheminski J, Groitl P, Dobner T et al. The adenoviral oncogene E1A-13S interacts with a specific isoform of the tumor suppressor PML to enhance viral transcription J Virol 2012 Nov 7 [PMID: 23135708] (ICC/IF, Human)
• Carracedo A, Weiss D, Leliaert AK et al. A metabolic prosurvival role for PML in breast cancer. J Clin Invest 2012 Sep [PMID: 22886304]
• Bade VN, Nickels J, Keusekotten K et al. Covalent protein modification with ISG15 via a conserved cysteine in the hinge region. PLoS One 2012 [PMID: 22693631]
• Chiantore MV, Vannucchi S, Accardi R et al. Interferon-beta induces cellular senescence in cutaneous human papilloma virus-transformed human keratinocytes by affecting p53 transactivating activity. PLoS One 2012 [PMID: 22615843]
• Sivachandran N, Wang X, Frappier L et al. Functions of the Epstein-Barr virus EBNA1 protein in viral reactivation and lytic infection. J Virol 2012 Jun [PMID: 22491455]
• Sivachandran N, Dawson CW, Young LS et al. Contributions of the Epstein-Barr virus EBNA1 protein to gastric carcinoma. J Virol 2012 Jan [PMID: 22013060]
• Everett RD. Study of early events during herpes simplex virus type 1 infection by confocal microscopy. Methods 2011 Oct [PMID: 21855636]
• Jung et al. The alphaherpesvirus serine/threonine kinase us3 disrupts promyelocytic leukemia protein nuclear bodies. J Virol. 2011 Jun [PMID: 21430051]
• Lee AK, Smart JL, Rubinstein M et al. Reciprocal regulation of TREK-1 channels by arachidonic acid and CRH in mouse corticotropes. Endocrinology 2011 May [PMID: 21343252] (Mouse)
• Sivachandran et al. J Virol 84 (21):11113-11123. 2010 [PMID: 20719947]
• Freudenberger N. Role of minor core protein V during productive infection with human adenovirus type 5 and the proteins relevance to oncogenic transformation processes Thesis 2017 (ICC/IF, Human)
• Wimmer P, Schreiner S, Everett RD et al. SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML. Oncogene 2010 Oct 7 [PMID: 20639899]

Review for PML Protein Antibody (NB100-59787) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/08/2011

Summary

• Application: Western Blot
• Sample Tested: Human
• Species: Human

Blocking

• Blocking Details: Blocking Buffer: 1% BSA

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:200, Primary Ab Incubation Time: overnight

Secondary Antibody

• Secondary Description: Secondary Ab: Anti-rabbit HRP

Details

• Detection Notes: Detection Method: Hyperfilm ECL

Bioinformatics

• Gene Symbol: PML
• Entrez:
  • 5371(Human)
• Uniprot:
  • P29590(Mouse)