PML Protein Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed ...read more
Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.
Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Western Blot: PML Protein Antibody [NB100-59787] - PML-RARa is a HIF-I+-transcriptional co-activator. HEK-293 cells transfected with stable mutants of HIF-1I+-or HIF-2I+-and increasing concentrations of PML-RARI+-. ...read more
Immunocytochemistry/ Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The ...read more
Immunocytochemistry/ Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PML used at a dilution of 1:500
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) ...read more
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm, PmSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-P, IP, PLA, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

PML Protein Antibody - BSA Free Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 375 and 425 of promyelocytic leukemia using the numbering given in entry NP_150241.2
Predicted Species
Primate (100%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PML
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Proximity Ligation Assay 1:200-1:5000
  • Western Blot 1:2000-1:10000
Application Notes
Immunohistochemistry 1:200 to 1:1,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in Immunoblotting reported in scientific literature (PMID 28239645). Knockdown and Flow Cytometry validation (PMID: 28402725).
Reviewed Applications
Read 1 Review rated 5
using
NB100-59787 in the following applications:

Publications
Read Publications using
NB100-59787 in the following applications:

  • 1 publication
  • 6 publications
  • IHC
    1 publication
  • IP
    1 publication
  • KD
    1 publication
  • WB
    9 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee, Primates, White-tufted-ear marmoset, Pygmy chimpanzee and Bornean orangutan. Mouse reactivity reported in scientific literature (PMID; 21343252)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PML Protein Antibody - BSA Free

  • MYLPP8675
  • Promyelocytic leukemia protein
  • promyelocytic leukemia
  • RING finger protein 71
  • RNF71probable transcription factor PML
  • TRIM19promyelocytic leukemia, inducer of
  • tripartite motif protein TRIM19
  • Tripartite motif-containing protein 19

Background

PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, IP, PLA, KD

Publications for PML Protein Antibody (NB100-59787)(34)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, IP, KD, WB.


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Flow
(1)
ICC/IF
(6)
IHC
(1)
IP
(1)
KD
(1)
WB
(9)
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Human
(13)
Mouse
(2)
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Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NB100-59787 Applications Species
Yan HY, Wang HQ, Zhong M et al. PML Suppresses Influenza Virus Replication by Promoting FBXW7 Expression Virologica Sinica May 27 2021 [PMID: 34046815]
Lee J, Stone J, Desai P et al. Arsenicals, the Integrated Stress Response, and Epstein-Barr Virus Lytic Gene Expression Viruses Apr 30 2021 [PMID: 33946406] (WB, Human) WB Human
Marks D, Heinen N, Bachmann L et al. Amyloid precursor protein elevates fusion of promyelocytic leukemia nuclear bodies in human hippocampal areas with high plaque load Acta neuropathologica communications Apr 13 2021 [PMID: 33849647] (IHC, Human) IHC Human
Karle W, Becker S, Stenzel P et al. Promyelocytic leukemia protein (PML) promotes the phenotypic switch of smooth muscle cells in atherosclerotic plaques of human coronary arteries Clinical science (London, England : 1979) Mar 25 2021 [PMID: 33764440]
Becker S, Steinemann G, Karle W et al. Stability of Smyd1 in endothelial cells is controlled by PML-dependent SUMOylation upon cytokine Stimulation Nature Nov 25 2020 [PMID: 33241844]
Grigore F, Yang H, Hanson ND et al. BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases Neoplasia Jul 3 2020 [PMID: 32629178]
Hofmann S, Mai J, Masser S et Al. ATO (Arsenic Trioxide) Effects on Promyelocytic Leukemia Nuclear Bodies Reveals Antiviral Intervention Capacity Adv. Sci. Feb 27 2020 [PMID: 32328411] (WB) WB
Stubbe M, Mai J, Paulus C et Al. Viral DNA Binding Protein SUMOylation Promotes PML Nuclear Body Localization Next to Viral Replication Centers mBio Mar 17 2020 [PMID: 32184235] (ICC/IF, Human) ICC/IF Human
Chen YT, Ou Yang WT, Juang HH et al. Proteomic characterization of arsenic and cadmium exposure in bladder cells Rapid Commun. Mass Spectrom. Sep 9 2019 [PMID: 31499585]
Li K, Wang F et al. TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RAR alpha and Inhibition of p53-Mediated Senescence. Cancer Cell Aug 5 2017 [PMID: 28486108] (WB, Mouse) WB Mouse
Show All 34 Publications.

Review for PML Protein Antibody (NB100-59787) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-59787:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Human 12/08/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 1% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:200, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-rabbit HRP

Details

Detection NotesDetection Method: Hyperfilm ECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PML Protein Antibody (NB100-59787) (0)

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Secondary Antibodies

 

Isotype Controls

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Verified Customer
12/08/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol PML
Entrez
Uniprot