PML Protein Antibody

Images

 
Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed ...read more
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PML used at a dilution of 1:500
Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) ...read more
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolin II PLA in Hela cells. goat anti-human HP1-beta (NB100-79774) and rabbit anti-human PML (NB100-59787). Image merged from DAPI ...read more
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) ...read more

Product Details

Summary
Reactivity Hu, Mu, Mk, PmSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PML Protein Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 375 and 425 of promyelocytic leukemia using the numbering given in entry NP_150241.2
Predicted Species
Primate (100%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PML
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:200-1:5000
Application Notes
Immunohistochemistry 1:200 to 1:1,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in Immunoblotting reported in scientific literature (PMID 28239645)..
Positive Control
PML Protein Lysate (NBP2-65281)
Reviewed Applications
Read 1 Review rated 5
using
NB100-59787 in the following applications:

Publications
Read Publications using
NB100-59787 in the following applications:

  • 5 publications
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee, Primates, White-tufted-ear marmoset, Pygmy chimpanzee and Bornean orangutan. Mouse reactivity reported in scientific literature (PMID; 21343252)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PML Protein Antibody

  • MYLPP8675
  • Promyelocytic leukemia protein
  • promyelocytic leukemia
  • RING finger protein 71
  • RNF71probable transcription factor PML
  • TRIM19promyelocytic leukemia, inducer of
  • tripartite motif protein TRIM19
  • Tripartite motif-containing protein 19

Background

PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-322
Species: Hu, Mu, Rt
Applications: WB, IHC-Fr
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-86060
Species: Hu
Applications: WB, IHC, IHC-P, Micro
NBP1-85309
Species: Hu
Applications: WB, IHC, IHC-P, KD
AF2944
Species: Hu
Applications: WB, Simple Western
NB100-381
Species: Hu
Applications: WB, ChIP, IP
NBP2-13809
Species: Hu
Applications: WB, IHC, IHC-P
AF5129
Species: Hu
Applications: WB
AF5414
Species: Hu
Applications: WB, Simple Western
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-89105
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00004830-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
AF755
Species: Mu
Applications: WB, Block
NBP2-55747
Species: Hu
Applications: WB, ICC/IF
NBP1-76729
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB500-487
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P, IP
NB100-59787
Species: Hu, Mu, Mk, Pm
Applications: WB, IB, ICC/IF, IHC, IHC-P, IP, PLA

Publications for PML Protein Antibody (NB100-59787)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(5)
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-59787 Applications Species
Yao K, Wu Y, Chen Q et al. The Arginine/Lysine-Rich Element within the DNA-Binding Domain Is Essential for Nuclear Localization and Function of the Intracellular Pathogen Resistance 1 PLoS ONE Sep 14 2016 [PMID: 27622275] (ICC/IF, Human) ICC/IF Human
Freudenberger N. Role of minor core protein V during productive infection with human adenovirus type 5 and the proteins relevance to oncogenic transformation processes Thesis 2017 (ICC/IF, Human) ICC/IF Human
Komatsu T, Nagata K, Wodrich H. An adenovirus DNA replication factor, but not incoming genome complexes, targets PML nuclear bodies. J. Virol. 2015 Nov 25 [PMID: 26608315]
Burck C, Mund A, Berscheminski J et al. KAP1 Is a Host Restriction Factor That Promotes Human Adenovirus E1B-55K SUMO Modification. J Virol 2015 Nov 04 [PMID: 26537675]
Berscheminski J, Brun J, Speiseder T et al. Sp100A is a tumor suppressor that activates p53-dependent transcription and counteracts E1A/E1B-55K-mediated transformation. Oncogene 2015 Oct 19 [PMID: 26477309]
Wimmer P, Berscheminski J, Blanchette P et al. PML isoforms IV and V contribute to adenovirus-mediated oncogenic transformation by functionally inhibiting the tumor-suppressor p53. Oncogene. 2015 Mar 16 [PMID: 25772236] (ICC/IF, Human) ICC/IF Human
Chen HY, Hu JY, Chen TH et al. KLHL39 suppresses colon cancer metastasis by blocking KLHL20-mediated PML and DAPK ubiquitination Oncogene. 2015 Jan 26 [PMID: 25619834] (WB) WB
Coltella N, Percio S, Valsecchi R et al. HIF factors cooperate with PML-RARalpha to promote acute promyelocytic leukemia progression and relapse. EMBO Mol Med 2014 Apr 07 [PMID: 24711541] (WB, IP, Human) WB, IP Human
Palibrk V, Lang E, Lang A et al. Promyelocytic leukemia bodies tether to early endosomes during mitosis. Cell Cycle 2014 [PMID: 24675887] (Human) Human
Berscheminski J, Wimmer P, Brun J et al. Sp100 isoform-specific regulation of human Adenovirus type 5 (Ad5) gene expression. J. Virol. 3/12/2014 [PMID: 24623443] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 22 Publications.

Review for PML Protein Antibody (NB100-59787) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-59787:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 12/08/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 1% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:200, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-rabbit HRP

Details

Detection NotesDetection Method: Hyperfilm ECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PML Protein Antibody (NB100-59787) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for PML Protein Antibody (NB100-59787)

Discover related pathways, diseases and genes to PML Protein Antibody (NB100-59787). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PML Protein Antibody (NB100-59787)

Discover more about diseases related to PML Protein Antibody (NB100-59787).
 

Pathways for PML Protein Antibody (NB100-59787)

View related products by pathway.

PTMs for PML Protein Antibody (NB100-59787)

Learn more about PTMs related to PML Protein Antibody (NB100-59787).
 

Research Areas for PML Protein Antibody (NB100-59787)

Find related products by research area.

Blogs on PML Protein

There are no specific blogs for PML Protein, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
12/08/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol PML
Entrez
Uniprot