Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed ...read more
Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.
Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Western Blot: PML Protein Antibody [NB100-59787] - PML-RARa is a HIF-I+-transcriptional co-activator. HEK-293 cells transfected with stable mutants of HIF-1I+-or HIF-2I+-and increasing concentrations of PML-RARI+-. ...read more
Immunocytochemistry/ Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The ...read more
Immunocytochemistry/ Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PML used at a dilution of 1:500
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) ...read more
Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) ...read more
The immunogen recognized by this antibody maps to a region between residue 375 and 425 of promyelocytic leukemia using the numbering given in entry NP_150241.2
Predicted Species
Primate (100%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PML
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunohistochemistry 1:200 to 1:1,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in Immunoblotting reported in scientific literature (PMID 28239645). Knockdown and Flow Cytometry validation (PMID: 28402725).
Reviewed Applications
Read 1 Review rated 5 using NB100-59787 in the following applications:
Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee, Primates, White-tufted-ear marmoset, Pygmy chimpanzee and Bornean orangutan. Mouse reactivity reported in scientific literature (PMID; 21343252)
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for PML Protein Antibody - BSA Free
MYLPP8675
Promyelocytic leukemia protein
promyelocytic leukemia
RING finger protein 71
RNF71probable transcription factor PML
TRIM19promyelocytic leukemia, inducer of
tripartite motif protein TRIM19
Tripartite motif-containing protein 19
Background
PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Freudenberger N. Role of minor core protein V during productive infection with human adenovirus type 5 and the proteins relevance to oncogenic transformation processes Thesis 2017 (ICC/IF, Human)
Bioinformatics Tool for PML Protein Antibody (NB100-59787)
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Diseases for PML Protein Antibody (NB100-59787)
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