TIF1 alpha Antibodies
We offer TIF1 alpha Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Western Blot. Each TIF1 alpha Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TIF1 alpha Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the TIF1 alpha Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TIF1 alpha polyclonal antibodies and browse our TIF1 alpha monoclonal antibody catalog.
Alternate Names for TIF1 alpha Antibodies
anti-TIF1 alpha antibody, anti-TRIM24 antibody, anti-E3 ubiquitin-protein ligase TRIM24 antibody, anti-EC 6.3.2 antibody, anti-EC 6.3.2.- antibody, anti-hTIF1 antibody, anti-PTC6 antibody, anti-RING finger protein 82 antibody, anti-RNF82Tif1a antibody, anti-TIF1-alpha antibody, anti-TIF1ATIF1ALPHA antibody, anti-TIF1TF1A antibody, anti-transcription intermediary factor 1-alpha antibody, anti-transcriptional intermediary factor 1 antibody, anti-tripartite motif containing 24 antibody, anti-tripartite motif-containing 24 antibody, anti-Tripartite motif-containing protein 24 antibody
20 Results for "tif1-alpha" in Primary Antibodies
![Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2. Analysis of TRIM24 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Western-Blot-H00008805-M01-img0011.jpg "Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2. Analysis of TRIM24 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Immunocytochemistry-Immunofluorescence-H00008805-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on formalin-fixed paraffin-embedded human testis. Antibody concentration 6 ug/ml.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Immunohistochemistry-Paraffin-H00008805-M01-img0008.jpg)
![Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2 Analysis of TRIM24 expression in IMR-32.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Western-Blot-H00008805-M01-img0010.jpg)
![Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of TRIM24 expression in transfected 293T cell line by TRIM24 monoclonal antibody (M01), clone 2F2.Lane 1: TRIM24 transfected lysate(116.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Western-Blot-H00008805-M01-img0012.jpg)
![Immunoprecipitation: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of TRIM24 transfected lysate using anti-TRIM24 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TRIM24 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2F2-Immunoprecipitation-H00008805-M01-img0009.jpg)
![Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 Alpha/TRIM24 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody NB100-2596 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NB100-2596-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 Alpha/TRIM24 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody NB100-2596 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-TIF1alpha/TRIM24 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-2596-img0013.jpg)
![Chromatin Immunoprecipitation Sequencing: TIF1 alpha Antibody [NB100-2596] - Localization of TIF1 Alpha/TRIM24 Binding Sites by ChIP-sequencing.Chromatin from Breast cancer cell line T47D was immunoprecipitated with anti-TIF1 Alpha/TRIM24 antibody NB100-2596 and analyzed by DNA sequencing. The figure illustrates the peak distribution of TIF1 Alpha/TRIM24 binding within a 250 Kb region of chromosome 19 as detected using anti-TIF1 Alpha/TRIM24 antibody NB100-2596. ChIP-seq validation performed by Active Motif, Carlsbad, CA.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB100-2596-img0014.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 alpha/TRIM24 on HeLa whole cell lysate using NB100-2596. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2597.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NB100-2596-img0003.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-2596-img0012.jpg)
![Western Blot: TIF1 alpha Antibody [NBP1-92506] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP1-92506-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TIF1 alpha Antibody [NBP1-92506] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody [NBP1-92506] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92506-img0006.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0012.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts while additional moderate cytoplasmic in Leydig cells.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0007.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP1-92506-img0010.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human placenta shows moderate nuclear and weak cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0011.jpg)
![Western Blot: TIF1 alpha Antibody [NB100-2597] - Detection of Human TIF1 alpha/TRIM24 on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-2597. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2596.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NB100-2597-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TIF1 alpha Antibody [NB100-2597] - Detection of Human TIF1 alpha/TRIM24 on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-2597. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2596.")
![Western Blot: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIF1.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Western-Blot-NBP2-46213-img0006.jpg "Western Blot: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIF1.")
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0005.jpg)
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0001.jpg)
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0002.jpg)
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0003.jpg)
![Immunohistochemistry: TIF1 alpha Antibody (2D9) [NBP2-46213] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-2D9-Immunohistochemistry-NBP2-46213-img0004.jpg)
![Western Blot: TIF1 alpha Antibody [NB300-847] - Analysis of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NB300-847-img0004.jpg "Western Blot: TIF1 alpha Antibody [NB300-847] - Analysis of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB300-847] - (4ug/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NB300-847-img0003.jpg)
![Western Blot: TIF1 alpha Antibody [NBP2-41220] - Western blot analysis of TRIM24 in EL4 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP2-41220-img0001.jpg "Western Blot: TIF1 alpha Antibody [NBP2-41220] - Western blot analysis of TRIM24 in EL4 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: TIF1 alpha Antibody - BSA Free [NBP2-41220] - Immunofluorescence of TIF1 alpha in EL4 cells with TIF1 alpha antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41220_rabbit-polyclonal-tif1-alpha-antibody-271120242017517.jpg)
![Immunocytochemistry/ Immunofluorescence: TIF1 alpha Antibody - BSA Free [NBP2-41220] - Immunocytochemistry of TIF1 alpha in EL4 cells with TIF1 alpha antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41220_rabbit-polyclonal-tif1-alpha-antibody-271120241946342.jpg)
![Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Immunofluorescent Analysis of PFA-fixed MCF7 cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by IgG-CF488 (green), counterstained with phalloidin.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-PCRP-TRIM24-1B12-Immunocytochemistry-Immunofluorescence-NBP3-07464-img0003.jpg "Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Immunofluorescent Analysis of PFA-fixed MCF7 cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by IgG-CF488 (green), counterstained with phalloidin.")
![Flow Cytometry: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-PCRP-TRIM24-1B12-Flow-Cytometry-NBP3-07464-img0002.jpg)
![Protein Array: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12).](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-PCRP-TRIM24-1B12-Protein-Array-NBP3-07464-img0001.jpg)
![Western Blot: TIF1 alpha Antibody [NBP2-56418] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP2-56418-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TIF1 alpha Antibody [NBP2-56418] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody [NBP2-56418] - Staining of human cell line Hep G2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56418-img0001.jpg)
![Western Blot: TIF1 alpha Antibody (S09-3G6) [NBP3-19977] - Western blot detection of TIF1 alpha in K562, Hela cell lysates using NBP3-19977 (1:1000 diluted). Predicted band size: 117kDa. Observed band size: 117kDa.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-S09-3G6-Western-Blot-NBP3-19977-img0001.jpg "Western Blot: TIF1 alpha Antibody (S09-3G6) [NBP3-19977] - Western blot detection of TIF1 alpha in K562, Hela cell lysates using NBP3-19977 (1:1000 diluted). Predicted band size: 117kDa. Observed band size: 117kDa.")
![Western Blot: TIF1 alpha Antibody (JE64-31) [NBP3-33052] - Western blot analysis of TIF1 alpha on different lysates with Rabbit anti-TIF1 alpha antibody at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 117 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody TRIM24 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33052_rabbit-tif1-alpha-mab-je64-31-288202412493491.jpg "Western Blot: TIF1 alpha Antibody (JE64-31) [NBP3-33052] - Western blot analysis of TIF1 alpha on different lysates with Rabbit anti-TIF1 alpha antibody at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 117 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody TRIM24 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TIF1 alpha Antibody (JE64-31) [NBP3-33052] - Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-TIF1 alpha antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody TRIM24 at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33052_rabbit-tif1-alpha-mab-je64-31-29820241411546.jpg)
![Western Blot: TIF1 alpha Antibody (9I0X1) [NBP3-33244] - Western blot analysis of various lysates using TIF1 alpha Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33244_rabbit-tif1-alpha-mab-9i0x1-10122024113603.jpg "Western Blot: TIF1 alpha Antibody (9I0X1) [NBP3-33244] - Western blot analysis of various lysates using TIF1 alpha Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")