RAR alpha/NR1B1 Antibody (OTI2C3)

Images

 
Western Blot: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAR alpha.
Immunohistochemistry: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 C for 3min)
Immunohistochemistry: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: RAR alpha/NR1B1 Antibody (2C3) [NBP2-45516] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2C3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

RAR alpha/NR1B1 Antibody (OTI2C3) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 52-299 of human RARA(NP_000955) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RARA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence - Reported in scientific literature (PMID: 30560112)
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
50.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-45516 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAR alpha/NR1B1 Antibody (OTI2C3)

  • alpha polypeptide
  • NR1B1
  • RAR alpha
  • RARA
  • RAR-alpha
  • retinoic acid receptor, alpha

Background

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAR alpha/NR1B1 Antibody (NBP2-45516)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for RAR alpha/NR1B1 Antibody (NBP2-45516) (0)

There are no reviews for RAR alpha/NR1B1 Antibody (NBP2-45516). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RAR alpha/NR1B1 Antibody (NBP2-45516) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional RAR alpha/NR1B1 Products

Research Areas for RAR alpha/NR1B1 Antibody (NBP2-45516)

Find related products by research area.

Blogs on RAR alpha/NR1B1

There are no specific blogs for RAR alpha/NR1B1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RAR alpha/NR1B1 Antibody (OTI2C3) and receive a gift card or discount.

Bioinformatics

Gene Symbol RARA