TIF1 alpha Antibody

Images

 
Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 Alpha/TRIM24 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared ...read more
Immunohistochemistry: TIF1 alpha Antibody [NB100-2596] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TIF1 alpha/TRIM24 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 alpha/TRIM24 on HeLa whole cell lysate using NB100-2596. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 ...read more
Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - FFPE section of human breast tumor. Antibody used at a dilution of 1:250.
Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:250.
Immunohistochemistry: TIF1 alpha Antibody [NB100-2596] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TIF1 alpha/TRIM24 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Proximity Ligation Assay: TIF1 alpha Antibody [NB100-2596] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HP1-beta (NB100-79774) and rabbit anti-human TRIM24 (NB100-2596). Image merged from DAPI ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

TIF1 alpha Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human Transcriptional Intermediary Factor 1 Alpha (Tripartite Motif-Containing 24 ) using the numbering given in entry NP_056989.2 (GeneID 8805).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM24
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-10 mg/mg
  • Proximity Ligation Assay 1:200-1:4000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
TIF1 alpha Lysate (NBL1-17284)
Publications
Read Publications using NB100-2596.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TIF1 alpha Antibody

  • E3 ubiquitin-protein ligase TRIM24
  • EC 6.3.2
  • EC 6.3.2.-
  • hTIF1
  • PTC6
  • RING finger protein 82
  • RNF82Tif1a
  • TIF1-alpha
  • TIF1ATIF1ALPHA
  • TIF1TF1A
  • transcription intermediary factor 1-alpha
  • transcriptional intermediary factor 1
  • tripartite motif containing 24
  • tripartite motif-containing 24
  • Tripartite motif-containing protein 24

Background

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIF1 alpha Antibody (NB100-2596)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIM24
Entrez
Uniprot