TIF1 alpha [p Ser1043] Antibody (7N5L6)

Western Blot: TIF1 alpha [p Ser1043] Antibody (7N5L6) [NBP3-33568] - Western blot analysis of lysates from HeLa cells, using TIF1 alpha Rabbit mAb at1:1000 dilution. Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 7N5L6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

TIF1 alpha [p Ser1043] Antibody (7N5L6) Summary

• Immunogen: A synthetic phosphorylated peptide around S1043 of mouse TIF1 alpha (NP_659542.3).
  
  Sequence:
  LKSTE
• Modification: p Ser1043
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 117 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for TIF1 alpha [p Ser1043] Antibody (7N5L6)

• E3 ubiquitin-protein ligase TRIM24
• EC 6.3.2
• EC 6.3.2.-
• hTIF1
• PTC6
• RING finger protein 82
• RNF82
• RNF82Tif1a
• TIF1 alpha
• TIF1
• TIF1A
• TIF1-alpha
• TIF1ATIF1ALPHA
• TIF1TF1A
• transcription intermediary factor 1-alpha
• transcriptional intermediary factor 1
• TRIM24
• tripartite motif containing 24
• tripartite motif-containing 24
• Tripartite motif-containing protein 24

Background

TIF1 alpha is encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.