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Mouse Tie-1 ELISA Kit (Colorimetric)

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Mouse Tie-1 - Standard Curve Reference
Mouse Tie-1 - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

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Mouse Tie-1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse ANG-R-Tie1 in samples. No significant cross-reactivity or interference between Mouse ANG-R-Tie1 and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.25%
Intra-Assay
CV% < 4.67%
Spike Recovery
87-102%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TIE1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Mouse Tie-1 ELISA Kit (Colorimetric)

  • EC 2.7.10
  • EC 2.7.10.1
  • JTK14soluble TIE1 variant 3
  • receptor tyrosine kinase
  • soluble TIE1 variant 1
  • soluble TIE1 variant 2
  • soluble TIE1 variant 5
  • Tie1
  • Tie-1
  • TIEsoluble TIE1 variant 4
  • tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1
  • tyrosine kinase with immunoglobulin-like and EGF-like domains 1
  • tyrosine-protein kinase receptor Tie-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Tie‑2 at approximately 161&amp;nbsp;kDa (as indicated) using 10&amp;nbsp;µg/mL of Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF313) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.
Tie‑2 was detected in immersion fixed paraffin-embedded sections of human placenta using&amp;nbsp;Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF313) at 10&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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VEGFR2 was detected in acetone fixed cryosections of mouse kidney tissue using Goat&amp;nbsp;Anti-Mouse VEGFR2/KDR/Flk-1 Polyclonal Antibody (Catalog # AF644) for 50 minutes&amp;nbsp;at room temperature. Tissues were stained with rabbit anti-goat secondaryantibody and HRP polymer-conjugated anti-rabbit IgG followed by AEC+Substrate&amp;nbsp;Chromogen (red) followed by counterstaining with hematoxylin (blue). Experiments were carried out and the image was provided by&amp;nbsp;Dr. Grietje Molema, University Medical Center Groningen, The Netherlands.
VEGFR2/KDR/Flk‑1 was detected in immersion fixed frozen sections of mouse embryo (14&amp;nbsp;d.p.c.) using 15&amp;nbsp;µg/mL Goat Anti-Mouse VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF644) overnight at 4&amp;nbsp;°C. Tissue was stained with the Anti-Goat HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Antibody [Unconjugate
AF743
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Western Blot: Menin Antibody [NB100-215] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Menin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.
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Mouse Tie-1 ELISA
Kit (Colorimetric)
NBP2-68259
Mouse Tie-1 ELISA Kit (Colorimetric)
Species: Mu
Applications: ELISA

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Bioinformatics

Gene Symbol TIE1