WIRE Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86856] - Analysis in human cerebral cortex and liver tissues using NBP1-86856 antibody. Corresponding WIRE RNA-seq data are presented for ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86856] - Staining of human cerebral cortex, colon, fallopian tube and liver using Anti-WIRE antibody NBP1-86856 (A) shows similar protein ...read more
Western Blot: WIRE Antibody [NBP1-86856] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86856] - Staining of human liver shows very weak positivyt in hepatocytes as expected.
Western Blot: WIRE Antibody [NBP1-86856] - Analysis using Anti-WIPF2 antibody NBP1-86856 (A) shows similar pattern to independent antibody NBP1-86858 (B).
Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86856] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86856] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: WIRE Antibody [NBP1-86856] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

WIRE Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PPPYRMHGSEPPSRGKPPPPPSRTPAGPPPPPPPPLRNGHRDSITTVRSFLDDFESKYSFHPVEDFPAPEEYKHFQ
Specificity
Specificity of human WIRE antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WIPF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
ICC/IF reported in scientific literature (PMID: 24413434). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
WIRE Protein (NBP1-86856PEP)
Publications
Read Publications using
NBP1-86856 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24413434)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for WIRE Antibody

  • PP10631
  • WAS/WASL interacting protein family, member 2
  • WIP-related protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WIRE Antibody (NBP1-86856)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WIRE Antibody (NBP1-86856) (0)

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Secondary Antibodies

 

Isotype Controls

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Additional WIRE Products

Bioinformatics Tool for WIRE Antibody (NBP1-86856)

Discover related pathways, diseases and genes to WIRE Antibody (NBP1-86856). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for WIRE Antibody (NBP1-86856)

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Calculators

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Bioinformatics

Gene Symbol WIPF2