Menin Antibody

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Biological Strategies: Western Blot: Menin Antibody [NB100-215] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: Menin Antibody [NB100-215] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Menin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

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Catalog# & Conjugate Size Price

Menin Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221).
Predicted Species
Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MEN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Menin Lysate (NBP2-64856)
Publications
Read Publications using NB100-215.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Menin Antibody

  • MEAI
  • MEN1
  • Menin
  • multiple endocrine neoplasia I
  • multiple

Background

Menin is the gene product of the putative tumor suppressor MEN1 associated with the syndrome termed multiple endocrine neoplasia type 1 that may function in the regulation of DNA synthesis or the response to DNA damage. Menin has been shown to interact with JunD, a member of the AP-1 heterodimer and inhibit transcription. Menin has also been shown to interact with Pem, FANCD2, RPA, NMMHCII-A, GFAP, vimentin, Hsp70, NfkappaB proteins, Smads, mSin3A and is part of the MLL2 and MLL1 Set1-like histone H3 K4 methyltransferase complex where it interacts directly with ASH2, Rbbp5, and WDR5. As part of the MLL complexes, menin may mediate its tumor suppressor activity via the regulation of histone methylation of HOX and CDK inhibitor genes. Alternate names for Menin are MEAI, multiple endocrine adenomatosis 1, suppressor Candidate Gene 2, and SCG2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IHC-P, IP

Publications for Menin Antibody (NB100-215)(26)


Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB100-215 Applications Species
Xu S, Wu H, Wang X et al. Tumor suppressor menin mediates peripheral nerve injury-induced neuropathic pain through potentiating synaptic plasticity. Neuroscience 2012 Oct [PMID: 22858595]
Veniaminova NA, Hayes MM, Varney JM et al. Conditional deletion of menin results in antral G cell hyperplasia and hypergastrinemia. Am J Physiol Gastrointest Liver Physiol 2012 Sep [PMID: 22766853]
Agarwal SK, Jothi R. Genome-wide characterization of menin-dependent H3K4me3 reveals a specific role for menin in the regulation of genes implicated in MEN1-like tumors. PLoS One 2012 [PMID: 22666422]
Niu J, Mei F, Wang L et al. Phosphorylated olig1 localizes to the cytosol of oligodendrocytes and promotes membrane expansion and maturation. Glia 2012 Sep [PMID: 22639060]
Wuescher L, Angevine K, Hinds T et al. Insulin regulates menin expression, cytoplasmic localization, and interaction with FOXO1. Am J Physiol Endocrinol Metab 2011 Sep [PMID: 21693693]
Corbo V, Dalai I, Scardoni M et al. MEN1 in pancreatic endocrine tumors: analysis of gene and protein status in 169 sporadic neoplasms reveals alterations in the vast majority of cases. Endocr Relat Cancer 2010 Aug [PMID: 20566584]
Shen HC, Ylaya K, Pechhold K et al. Multiple endocrine neoplasia type 1 deletion in pancreatic alpha-cells leads to development of insulinomas in mice. Endocrinology 2010 Aug [PMID: 20555035]
Wu T, Zhang X, Huang X et al. Regulation of cyclin B2 expression and cell cycle G2/m transition by menin. J Biol Chem 2010 Jun [PMID: 20404349]
Shen HC, He M, Powell A et al. Recapitulation of pancreatic neuroendocrine tumors in human multiple endocrine neoplasia type I syndrome via Pdx1-directed inactivation of Men1. Cancer Res 2009 Mar [PMID: 19208834]
Gatta R, Mantovani R. NF-Y substitutes H2A-H2B on active cell-cycle promoters: recruitment of CoREST-KDM1 and fine-tuning of H3 methylations. Nucleic Acids Res 2008 Nov [PMID: 18940868]
Show All 26 Publications.

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Bioinformatics

Gene Symbol MEN1
Entrez
Uniprot