TGF-beta 3 Antibody (44922)

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="TGF-beta 3 was detected in immersion fixed paraffin-embedded sections of human heart using TGF-beta 3 Monoclonal Antibody (Catalog # MAB643) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

TGF-beta 3 was detected in immersion fixed paraffin-embedded sections of human heart using TGF-beta 3 Monoclonal Antibody (Catalog # MAB643) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

243-B3) was serially diluted and captured by Mouse Anti-TGF‑ beta 3 Monoclonal Antibody (Catalog # MAB643) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-TGF‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 243-B3) was serially diluted and captured by Mouse Anti-TGF‑ beta 3 Monoclonal Antibody (Catalog # MAB643) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-TGF‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human TGF‑ beta 3 (Catalog # 243-B3) was serially diluted and captured by Mouse Anti-TGF‑ beta 3 Monoclonal Antibody (Catalog # MAB643) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-TGF‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human TGF‑ beta 3 (Catalog # <a class=

• Reactivity: All-Multi
• Applications: WB, IHC, ELISA(Cap)
• Clone: 44922
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TGF-beta 3 Antibody (44922) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TGF‑ beta 3
  Ala301-Ser412 (Tyr340Phe)
  Accession # NP_003230.1
• Specificity: Detects TGF-beta 3 from multiple species in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) TGF-beta 1or rhTGF-beta 2.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFB3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: TGF-beta 3 Biotinylated Antibody (Catalog number BAF243)
  Standard: Recombinant Human TGF-beta 3 (Catalog number 243-B3)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 3 Antibody (44922)

• ARVD
• ARVD1
• FLJ16571
• LDS5
• RNHF
• TGFB3
• TGFbeta 3
• TGF-beta 3
• TGF-beta3
• TGF-beta-3
• transforming growth factor beta-3
• transforming growth factor, beta 3

Background

The active form of TGF-beta 3 is a disulfide-linked homodimer which is generated by activation of the latent TGF-beta 3 complex. TGF-beta s are multifunctional cytokines that activate or repress multiple genes in a wide variety of cell types. Depending on the cell context, TGF-beta can control many cellular processes including growth, morphogenesis, and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta 3 Antibody (MAB643)(5)

Publications using MAB643

• Albawaana, A;Day, A;Lu, H; The Impact of Cysteine Substitutions on TGF-?3 Expression, Purification, Folding, and Activity International journal of molecular sciences 2026-03-06 [PMID: 41828639] (Western Blot, Human)
• Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis. Nat Commun, 2016-03-18;7(0):11012. 2016-03-18 [PMID: 26987776] (Western Blot, Human)
• Kameswara Rao Badri, Ming Yue, Oscar A. Carretero, Sree Latha Aramgam, Jun Cao, Stephen Sharkady et al. Blood pressure homeostasis is maintained by a P311–TGF-beta axis Journal of Clinical Investigation 10/1/2013 [PMID: 24091331]
• Kameswara Rao Badri, Ming Yue, Oscar A. Carretero, Sree Latha Aramgam, Jun Cao, Stephen Sharkady et al. Blood pressure homeostasis is maintained by a P311–TGF-beta axis Journal of Clinical Investigation 2013-10-01 [PMID: 24091331] (ELISA Capture, Mouse)
• Simpson H, 2019, Robson SC, Bulmer JN, Barber A, Lyall F Transforming growth factor beta expression in human placenta and placental bed during early pregnancy. 6897, 2002-01-01;23(1):44-58. 2002-01-01 [PMID: 11869091] (Human)

Bioinformatics

• Gene Symbol: TGFB3
• Uniprot:
  • NP_003230.1()