Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF ...read more
Immunohistochemistry: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong ...read more
Expect a band approximately 48 kDa in size corresponding - phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
Read 1 Review rated 3 using NBP1-77836 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Immunogen affinity purified
Alternate Names for Smad3 [p Ser423, p Ser425] Antibody
MAD homolog 3
mad protein homolog
MAD, mothers against decapentaplegic homolog 3 (Drosophila)
MAD, mothers against decapentaplegic homolog 3
MADH3mad homolog JV15-2
mothers against decapentaplegic homolog 3
Mothers against DPP homolog 3
SMA- and MAD-related protein 3
SMAD family member 3HSPC193
SMAD, mothers against DPP homolog 3 (Drosophila)
SMAD, mothers against DPP homolog 3
Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
K. Bhawal U, Lee H, Uchida R, Okumura S. The Pro-Healing Effect of Protamine-Hydrolysate Peptides on Skin Wounds Involves TGF-beta/Smad Signaling Journal of Hard Tissue Biology. 2015 Jan 20 (IHC-P, Rat)
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