Smad3 [p Ser423, p Ser425] Antibody

Images

 
Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF ...read more
Immunohistochemistry: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Xp, ZeSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, Single Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

Order Details

Smad3 [p Ser423, p Ser425] Antibody Summary

Immunogen
Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein.
Modification
p Ser423, p Ser425
Specificity
This antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:20000
  • ELISA 1:15000 - 1:30000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Single Cell Western 100 ug/ml
Application Notes
Expect a band approximately 48 kDa in size corresponding - phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-77836 in the following applications:

Publications
Read Publications using
NBP1-77836 in the following applications:

Reactivity Notes

Xenopus laevis, Xenopus tropicalis.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Smad3 [p Ser423, p Ser425] Antibody

  • DKFZp586N0721
  • DKFZp686J10186
  • hMAD-3
  • hSMAD3
  • HsT17436
  • JV15-2MGC60396
  • MAD homolog 3
  • mad protein homolog
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • MAD, mothers against decapentaplegic homolog 3
  • mad3
  • MADH3mad homolog JV15-2
  • mothers against decapentaplegic homolog 3
  • Mothers against DPP homolog 3
  • pSMAD
  • SMA- and MAD-related protein 3
  • SMAD 3
  • SMAD family member 3HSPC193
  • SMAD, mothers against DPP homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3
  • Smad3

Background

Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, IHC, IHC-P, Single Cell Western

Publications for Smad3 Antibody (NBP1-77836)(4)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, IHC-P.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
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Mouse
(1)
Rat
(1)
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Showing Publications 1 - 4 of 4.
Publications using NBP1-77836 Applications Species
Yamashita M, Fatyol K, Jin C et al. TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta. Mol Cell 2008 Sep [PMID: 18922473]
K. Bhawal U, Lee H, Uchida R, Okumura S. The Pro-Healing Effect of Protamine-Hydrolysate Peptides on Skin Wounds Involves TGF-beta/Smad Signaling Journal of Hard Tissue Biology. 2015 Jan 20 (IHC-P, Rat) IHC-P Rat
Rogers KA, Moreno SE, Smith LA et al. Differences in the timing and magnitude of Pkd1 gene deletion determine the severity of polycystic kidney disease in an orthologous mouse model of ADPKD Physiol Rep 2016 Jun 01 [PMID: 27356569]
Huber AK, Sorkin M, Hwang C, et al. Regulation of heterotopic ossification through local inflammatory monocytes in a mouse model of aberrant wound healing bioRxiv (ICC/IF, Mouse) ICC/IF Mouse

Review for Smad3 Antibody (NBP1-77836) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Feline.

Reviews using NBP1-77836:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Smad3 NBP1-77836
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reviewed by:
Anonymous
IHC-Fr Feline 08/15/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRetina
SpeciesFeline
Lot33559

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Smad3 Antibody (NBP1-77836). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
    • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77836AF350
Alexa Fluor 405 NBP1-77836AF405
Alexa Fluor 488 NBP1-77836AF488
Alexa Fluor 532 NBP1-77836AF532
Alexa Fluor 594 NBP1-77836AF594
Alexa Fluor 647 NBP1-77836AF647
Alexa Fluor 700 NBP1-77836AF700
Alexa Fluor 750 NBP1-77836AF750
Allophycocyanin NBP1-77836APC
Biotin NBP1-77836B
DyLight 350 NBP1-77836UV
DyLight 405 NBP1-77836V
DyLight 488 NBP1-77836G
DyLight 550 NBP1-77836R
DyLight 594 NBP1-77836DL594
DyLight 650 NBP1-77836C
DyLight 680 NBP1-77836FR
DyLight 755 NBP1-77836IR
FITC NBP1-77836F
HRP NBP1-77836H
Janelia Fluor 549 NBP1-77836JF549
Janelia Fluor 646 NBP1-77836JF646
PE NBP1-77836PE
PerCP NBP1-77836PCP

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Recent Reviews

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5
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1
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Anonymous
08/15/2017
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol SMAD3
Uniprot
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