TGF-beta RII Antibody [Unconjugated]

Images

 
TGF-beta RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight ...read more
Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta RII Antigen Affinity-purified Poly­clonal Antibody (Catalog #AF‑241‑NA, filled histogram) or control antibody (Catalog #AB‑108‑C, ...read more
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell ...read more
Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Human IL‑4 (Catalog # 204-IL) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

TGF-beta RII Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RII
Ile24-Asp159
Accession # P37173.2
Specificity
Detects human TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse
TGF‑ beta  RII is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFBR2
Endotoxin Note
<0.03 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Flow Cytometry 1 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 5-20 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human TGF-beta RII Antibody (Catalog # AF-241-NA)
ELISA Detection: Human TGF-beta RII Biotinylated Antibody (Catalog # BAF241)
Standard: Recombinant Human TGF-beta RII (Catalog # 241-R2)
Positive Control
TGF-beta RII Lysate (NBP2-64883)
Publications
Read Publications using
AF-241-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RII Antibody [Unconjugated]

  • AAT3
  • EC 2.7.11
  • EC 2.7.11.30
  • FAA3
  • HNPCC6
  • LDS1B
  • LDS2B
  • MFS2
  • RIIC
  • TAAD2
  • tbetaR-II
  • TGF-beta receptor type II
  • TGF-beta receptor type IIB
  • TGF-beta receptor type-2
  • TGF-beta RII
  • TGF-beta type II receptor
  • TGFbetaRII
  • TGFbeta-RII
  • TGFBR2
  • TGF-bRII
  • TGFR-2
  • transforming growth factor beta receptor type IIC
  • transforming growth factor, beta receptor II (70/80kDa) isoform 1
  • transforming growth factor, beta receptor II (70/80kDa) isoform 2
  • transforming growth factor, beta receptor II (70/80kDa)
  • transforming growth factor, beta receptor II (70-80kD)
  • Transforming growth factor-beta receptor type II

Background

TGF beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF beta RII interacts with TGF beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, IHC, B/N, CyTOF-ready, ELISA(Cap)

Publications for TGF-beta RII Antibody (AF-241-NA)(16)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Feline, Rabbit.

We have publications tested in 7 applications: Bioassay, Blocking, IHC, IHC Paraffin-embedded, In vivo, Neut, WB.


Filter By Application
Bioassay
(1)
Blocking
(1)
IHC
(2)
IHC Paraffin-embedded
(1)
In vivo
(1)
Neut
(9)
WB
(1)
All Applications
Filter By Species
Human
(9)
Mouse
(3)
Rat
(2)
Feline
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF-241-NA Applications Species
OE Olsen, A Skjærvik, BF Størdal, A Sundan, T Holien TGF-? contamination of purified recombinant GDF15 PLoS ONE, 2017;12(11):e0187349. 2017 [PMID: 29161287] (Neut,, Human) Neut Human
24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats PLoS ONE, 2016;11(8):e0161782. 2016 [PMID: 27575371] (Blocking,, Rat) Blocking Rat
Clathrin light chains&#039; role in selective endocytosis influences antibody isotype switching Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27540116] (Bioassay, Human) Bioassay Human
Kenny H, Chiang C, White E, Schryver E, Habis M, Romero I, Ladanyi A, Penicka C, George J, Matlin K, Montag A, Wroblewski K, Yamada S, Mazar A, Bowtell D, Lengyel E Mesothelial cells promote early ovarian cancer metastasis through fibronectin secretion. J Clin Invest, 2014;124(10):4614-28. 2014 [PMID: 25202979] (Neut,, Human) Neut Human
Gate D, Danielpour M, Rodriguez J, Kim G, Levy R, Bannykh S, Breunig J, Kaech S, Flavell R, Town T T-cell TGF-beta signaling abrogation restricts medulloblastoma progression. Proc Natl Acad Sci U S A, 2014;111(33):E3458-66. 2014 [PMID: 25082897] (IHC,, Human) IHC Human
Chen X, Wang H, Liao H, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang Z, Vega-Montoto L, Vanacore R, Fassler R, Zent R, Pozzi A Integrin-mediated type II TGF-beta receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling. J Clin Invest, 2014;124(8):3295-310. 2014 [PMID: 24983314] (Neut,, Mouse) Neut Mouse
Miller M, Petty C, Tompkins M, Fogle J CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFbeta / GARP-mediated mechanism. Virol J, 2014;11(0):7. 2014 [PMID: 24438223] (Neut,, Feline) Neut Feline
Allen M, Thomas G, Clark S, Dawoud M, Vallath S, Payne S, Gomm J, Dreger S, Dickinson S, Edwards D, Pennington C, Sestak I, Cuzick J, Marshall J, Hart I, Jones J Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of alphavbeta6 integrin in DCIS identifies high-risk patients and predicts recurrence. Clin Cancer Res, 2014;20(2):344-57. 2014 [PMID: 24150233] (Neut,, Mouse) Neut Mouse
Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells. Cancer Res, 2013;73(7):2333-44. 2013 [PMID: 23365134] (WB,, Human) WB Human
Rai D, Kim SW, McKeller MR Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis. Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3111-6. 2010 [PMID: 20133617] (Neut,, Human) Neut Human
Show All 16 Publications.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TGFBR2
Entrez
Uniprot